Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   RSP597_RS13515 Genome accession   NZ_CP172969
Coordinates   2829025..2830290 (-) Length   421 a.a.
NCBI ID   WP_064044961.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain P597     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2824025..2835290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP597_RS13485 (RSP597_013480) - 2825088..2825972 (+) 885 WP_013205089.1 ATP-binding protein -
  RSP597_RS13490 (RSP597_013485) - 2825950..2826411 (+) 462 WP_042590562.1 NUDIX domain-containing protein -
  RSP597_RS13495 (RSP597_013490) yacG 2826404..2826622 (-) 219 WP_013205091.1 DNA gyrase inhibitor YacG -
  RSP597_RS13500 (RSP597_013495) zapD 2826638..2827396 (-) 759 WP_013205092.1 cell division protein ZapD -
  RSP597_RS13505 (RSP597_013500) coaE 2827514..2828128 (-) 615 WP_064044960.1 dephospho-CoA kinase -
  RSP597_RS13510 (RSP597_013505) pilD 2828141..2829022 (-) 882 WP_042590560.1 A24 family peptidase Machinery gene
  RSP597_RS13515 (RSP597_013510) pilG 2829025..2830290 (-) 1266 WP_064044961.1 type II secretion system F family protein Machinery gene
  RSP597_RS13520 (RSP597_013515) pilB 2830335..2832071 (-) 1737 WP_064044962.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RSP597_RS13525 (RSP597_013520) - 2832319..2833596 (-) 1278 WP_042590559.1 HlyC/CorC family transporter -
  RSP597_RS13535 (RSP597_013530) ispB 2833872..2834840 (-) 969 WP_081262488.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46483.58 Da        Isoelectric Point: 10.3648

>NTDB_id=1069773 RSP597_RS13515 WP_064044961.1 2829025..2830290(-) (pilG) [Ralstonia solanacearum strain P597]
MATRAPTANRSAAASARKTASTKAPTQYIFEWEGKDRKGKAFKGEMRAENITEVNAVLRKQGLSITKSKRRRAARGKKIT
PKDVAYFTRQLSTMLKAGVPLLQSVDIIARGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDSLYCNLIDAGEQGG
ILDALLERLSMYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKNVFASFGATLPAPTLVVMNISDFFVKW
WYIIIFAPAVGIFLYARAFKRSENVQRNTHKAILKMPIFGSIIRKATIARWTRTLATMFSAGTPLVESMESVAGAAGNWV
YHDATLEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=1069773 RSP597_RS13515 WP_064044961.1 2829025..2830290(-) (pilG) [Ralstonia solanacearum strain P597]
ATGGCCACACGAGCACCGACCGCGAACCGGTCCGCCGCCGCATCGGCACGCAAAACTGCCTCCACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAGGACCGCAAAGGCAAAGCCTTCAAGGGCGAGATGCGGGCGGAGAACATCACCGAAG
TCAACGCGGTGCTGCGCAAGCAGGGGCTGTCGATCACCAAGTCCAAGCGTCGGCGCGCGGCGCGCGGCAAGAAGATCACG
CCCAAGGACGTGGCCTACTTCACGCGCCAGCTGTCGACCATGCTGAAGGCCGGGGTGCCGCTGCTGCAATCGGTCGACAT
CATCGCGCGCGGCCACGCCAACCCGAACTTCACCCAACTGCTGACGGAAATCCGTGTCGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCCTTCCGCCGCCATCCGAAGTACTTCGATTCGCTGTACTGCAACCTGATCGACGCGGGCGAACAGGGCGGT
ATTCTCGACGCGCTGCTCGAGCGGCTGTCGATGTACATGGAAAAATCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTACCCAATCTCGGTGCTGACGGTGGCCTTCCTGGTCACGGTGGTGCTGATGATCTTCGTGGTGCCCGCGTTCAAGA
ACGTGTTCGCCAGCTTCGGCGCCACGCTGCCGGCACCGACGCTGGTGGTGATGAATATCTCAGACTTCTTCGTGAAGTGG
TGGTACATCATCATCTTTGCCCCCGCCGTCGGTATCTTCCTGTATGCGCGGGCCTTCAAGCGCTCGGAGAACGTGCAGCG
CAACACGCACAAGGCCATCCTGAAGATGCCGATCTTCGGCAGCATCATCCGCAAGGCCACCATCGCGCGCTGGACGCGCA
CGCTGGCGACGATGTTCTCCGCCGGTACGCCGCTGGTGGAATCGATGGAGTCGGTGGCCGGTGCGGCCGGCAACTGGGTC
TACCACGACGCCACCCTCGAGATCCAGCAGGCGGTACGCATCGGTACCAGCCTCACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGCGAGGAATCCGGTGCGCTGGACAACATGCTGCTGAAGGTGGCCG
AGTTCTACGAGCGCGAGGTCGATGACGCGGTAGCCAACATCTCCGCCCTGATCGAGCCGATCATCATCGTGATCCTGGGC
GTGCTGATCGGCGGCATGGTGGTCGCCATGTACCTGCCGATCTTCAAGCTGGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

50.877

94.774

0.482
  pilG Neisseria meningitidis 44/76-A

50.627

94.774

0.48
  pilC Pseudomonas stutzeri DSM 10701

49.254

95.487

0.47
  pilC Acinetobacter baumannii D1279779

47.42

96.675

0.458
  pilC Acinetobacter baylyi ADP1

46.683

96.675

0.451
  pilC Legionella pneumophila strain ERS1305867

46.02

95.487

0.439
  pilC Vibrio campbellii strain DS40M4

39.012

96.2

0.375
  pilC Vibrio cholerae strain A1552

39

95.012

0.371

Multiple sequence alignment