Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   RSP597_RS13520 Genome accession   NZ_CP172969
Coordinates   2830335..2832071 (-) Length   578 a.a.
NCBI ID   WP_064044962.1    Uniprot ID   -
Organism   Ralstonia solanacearum strain P597     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2825335..2837071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RSP597_RS13490 (RSP597_013485) - 2825950..2826411 (+) 462 WP_042590562.1 NUDIX domain-containing protein -
  RSP597_RS13495 (RSP597_013490) yacG 2826404..2826622 (-) 219 WP_013205091.1 DNA gyrase inhibitor YacG -
  RSP597_RS13500 (RSP597_013495) zapD 2826638..2827396 (-) 759 WP_013205092.1 cell division protein ZapD -
  RSP597_RS13505 (RSP597_013500) coaE 2827514..2828128 (-) 615 WP_064044960.1 dephospho-CoA kinase -
  RSP597_RS13510 (RSP597_013505) pilD 2828141..2829022 (-) 882 WP_042590560.1 A24 family peptidase Machinery gene
  RSP597_RS13515 (RSP597_013510) pilG 2829025..2830290 (-) 1266 WP_064044961.1 type II secretion system F family protein Machinery gene
  RSP597_RS13520 (RSP597_013515) pilB 2830335..2832071 (-) 1737 WP_064044962.1 type IV-A pilus assembly ATPase PilB Machinery gene
  RSP597_RS13525 (RSP597_013520) - 2832319..2833596 (-) 1278 WP_042590559.1 HlyC/CorC family transporter -
  RSP597_RS13535 (RSP597_013530) ispB 2833872..2834840 (-) 969 WP_081262488.1 octaprenyl diphosphate synthase -
  RSP597_RS13540 (RSP597_013535) rplU 2835118..2835429 (+) 312 WP_003271629.1 50S ribosomal protein L21 -
  RSP597_RS13545 (RSP597_013540) rpmA 2835467..2835727 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  RSP597_RS13550 (RSP597_013545) obgE 2835925..2837025 (+) 1101 WP_003271633.1 GTPase ObgE -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 64117.93 Da        Isoelectric Point: 7.1547

>NTDB_id=1069774 RSP597_RS13520 WP_064044962.1 2830335..2832071(-) (pilB) [Ralstonia solanacearum strain P597]
MTLGLALTQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSANKHF
QQLRLLPLGRRDNRIIVATSDPSDPKPIEELRQKMNVAIETVIVEHDKLMRQLSVANEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKDNKETVERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQL
LLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPK
ETLLDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILTHGTALQIAEQARKEGVLSLRDSGLR
KVKQGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1069774 RSP597_RS13520 WP_064044962.1 2830335..2832071(-) (pilB) [Ralstonia solanacearum strain P597]
ATGACTCTCGGACTCGCACTTACGCAGAGCCGGCGAATTGCGCCGACATTACTCACTCAGCTAGAGCAGAGCGCGCGCGA
AAAGCGCACGCAGTTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCGCACGACCTCGCCGTCTTCGTGGCGGGCA
AGTACCAGATGCCGCTGCTGGACCTGGCGCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGGCCAACAAGCATTTC
CAGCAGTTGCGCCTGCTGCCGCTGGGCCGGCGCGACAACCGCATCATCGTCGCCACCTCGGACCCGAGCGACCCGAAGCC
GATCGAAGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGATGCGCCAGTTGA
GCGTCGCCAACGAAGCGCCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACCCAGATCGAATACGACCCGGGC
GCGGCCGCAGCGGTGCGGCGTACCCCGACCGACGGCATCGACGATGCACCGGTGGTGCGCTTCCTGCAGAAGCTGTTCAC
CGAGGCGCTGCTGCGCGGCACGTCCGACTTGCACTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTTGAACAAGTCGCTCAGCCGCCGCTGGACATCCGCGACAAGATCGCTACCCGTATCAAGGTGTTGTCGCGCCTG
GACATCTCGGAAAAACGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCACCGTTCCGAAGGACAAGAACGACAAGGA
CAACAAGGACAACAAGGAGACGGTCGAGCGCGCTATCGACTTCCGGGTATCGACGCTGCCGACGCTGTTCGGCGAGAAGA
TCGTGATCCGGATTCTGGAGTCCTCGACCGAGCGGCTCGATATCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTC
CTGCTGGACGTGATCAAACGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTA
CACCTTCCTGAACAGGCTGAACCAGGGCGACATCAACATTTCCACGGCGGAAGATCCGGCGGAAATCCAGTTGCCCGGCA
TCAACCAGGTCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAATCGTTCCTGCGTCAGGATCCCGAC
ATCATCATGGTGGGCGAAATCCGGGACCTGGAAACCGCCGATATCTCCATCAAGGCCGCACAGACCGGCCACTTGGTGTT
CTCCACGCTGCACACCAACGACGCGCCGACCACGCTCACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCCT
CGTCGGTGCTGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGCCCGCTGCCCAAG
GAAACGCTGCTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGC
ATGCAACGGCAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCC
TCACCCACGGCACCGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAGGGCGTGCTCTCGCTGCGCGACTCCGGCCTGAGA
AAAGTGAAGCAGGGGCTGACTTCCCTTGAGGAAGTGCTGGCCACGACGAACCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.142

98.443

0.503
  pilF Neisseria gonorrhoeae MS11

52.641

94.983

0.5
  pilB Acinetobacter baylyi ADP1

49.472

98.27

0.486
  pilB Legionella pneumophila strain ERS1305867

46.062

100

0.465
  pilB Vibrio cholerae strain A1552

44.405

97.405

0.433
  pilB Vibrio campbellii strain DS40M4

44.646

95.329

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

44.607

94.637

0.422
  pilB/pilB1 Synechocystis sp. PCC 6803

36.333

100

0.377
  pilF Thermus thermophilus HB27

38.078

97.232

0.37

Multiple sequence alignment