Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AADG46_RS09845 Genome accession   NZ_AP028804
Coordinates   2178287..2179513 (+) Length   408 a.a.
NCBI ID   WP_000648507.1    Uniprot ID   -
Organism   Vibrio cholerae strain VN152     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2173287..2184513
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADG46_RS09820 (VNVC001_18620) fldB 2173520..2174038 (+) 519 WP_000690112.1 flavodoxin FldB -
  AADG46_RS09825 (VNVC001_18630) ampD 2174169..2174714 (-) 546 WP_340701785.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AADG46_RS09830 (VNVC001_18640) nadC 2175010..2175900 (+) 891 WP_114743264.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AADG46_RS09835 (VNVC001_18650) - 2176145..2176549 (+) 405 WP_000649343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AADG46_RS09840 (VNVC001_18660) pilB 2176556..2178244 (+) 1689 WP_148503289.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AADG46_RS09845 (VNVC001_18670) pilC 2178287..2179513 (+) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  AADG46_RS09850 (VNVC001_18680) pilD 2179571..2180446 (+) 876 WP_000418753.1 A24 family peptidase Machinery gene
  AADG46_RS09855 (VNVC001_18690) coaE 2180443..2181051 (+) 609 WP_000011551.1 dephospho-CoA kinase -
  AADG46_RS09860 (VNVC001_18700) zapD 2181083..2181823 (+) 741 WP_000207194.1 cell division protein ZapD -
  AADG46_RS09865 (VNVC001_18710) yacG 2181981..2182178 (+) 198 WP_000162866.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 45104.41 Da        Isoelectric Point: 10.3424

>NTDB_id=106918 AADG46_RS09845 WP_000648507.1 2178287..2179513(+) (pilC) [Vibrio cholerae strain VN152]
MKATQTLPLKNYRWKGINSNGKKVSGQMLAISEIEVRDKLKDQHIQIKKLKKGSVSLLARLTHRVKGKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKSVEAGTPLSKAMRTASAHFDTLYVDLVETGEMSGNLPEVFERLATYRE
KSEQLRAKVIKALIYPSMVVLVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQVLKLSHWVQAYSLWAFIAIAAAIFG
LKALRKNSFQIRLKTSRLGLKFPIIGNVLAKASIAKFSRTLATSFAAGIPILASLKTTAKTSGNVHFETAINEVYRDTAA
GMPMYIAMRNTEAFPEMVLQMVMIGEESGQLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=106918 AADG46_RS09845 WP_000648507.1 2178287..2179513(+) (pilC) [Vibrio cholerae strain VN152]
ATGAAAGCGACCCAAACCTTACCTCTGAAAAATTATCGCTGGAAAGGCATCAACAGCAACGGCAAAAAAGTTTCCGGCCA
GATGCTCGCCATCTCCGAAATCGAGGTGCGCGATAAGCTCAAAGATCAGCATATTCAGATCAAAAAACTCAAAAAAGGCA
GTGTCTCTTTATTGGCGCGCCTAACCCATCGCGTGAAAGGCAAAGACATCACGATTTTGACTCGGCAGTTGGCGACCATG
CTCACCACGGGCGTACCGATTGTGCAAGCCCTCAAGTTGGTGGGCGATAATCACCGTAAAGCTGAGATGAAATCGATTCT
GGCGCAAATCACCAAAAGTGTGGAAGCGGGCACGCCGCTTTCCAAGGCGATGCGCACCGCCAGCGCCCATTTTGATACCT
TGTATGTCGACTTAGTGGAAACCGGAGAGATGTCCGGCAACTTACCTGAGGTGTTTGAGCGTTTGGCCACCTACCGCGAG
AAAAGCGAGCAGCTACGCGCCAAGGTGATTAAAGCGCTCATCTACCCCAGCATGGTGGTGCTGGTCGCGCTCGGGGTATC
TTACTTAATGCTCACCATGGTCATCCCAGAATTTGAAAGCATGTTCAAAGGCTTTGGTGCAGAACTGCCTTGGTTTACGC
AGCAAGTGCTGAAACTCTCACACTGGGTGCAGGCTTACAGTTTATGGGCATTTATCGCCATTGCAGCAGCCATTTTTGGC
TTGAAAGCGCTGCGTAAAAACTCCTTCCAGATCCGTTTAAAAACCAGTCGATTAGGGCTGAAATTTCCGATTATTGGTAA
TGTGCTCGCCAAAGCGTCCATCGCCAAATTCAGCCGCACCCTCGCCACCAGCTTTGCCGCGGGGATCCCGATCCTCGCCA
GTTTAAAAACCACCGCCAAAACCTCCGGCAATGTGCACTTTGAAACCGCGATTAATGAGGTGTACCGCGATACCGCTGCG
GGTATGCCGATGTACATTGCGATGCGCAATACTGAAGCTTTTCCGGAAATGGTGCTGCAAATGGTGATGATCGGTGAAGA
GTCTGGGCAATTAGATGACATGCTCAACAAGGTCGCGACCATCTATGAATTTGAAGTCGATAACACAGTCGATAACTTGG
GCAAGATTCTTGAACCACTGATCATCGTCTTTCTTGGGACGGTCGTGGGCGGCTTAGTGGTGGCGATGTACTTACCTATC
TTTAACTTAATGAGTGTATTGGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

99.755

100

0.998
  pilC Vibrio campbellii strain DS40M4

74

98.039

0.725
  pilC Acinetobacter baumannii D1279779

42.647

100

0.426
  pilC Acinetobacter baylyi ADP1

42.015

99.755

0.419
  pilC Legionella pneumophila strain ERS1305867

42.211

97.549

0.412
  pilC Pseudomonas stutzeri DSM 10701

42.172

97.059

0.409
  pilG Neisseria gonorrhoeae MS11

41.294

98.529

0.407
  pilG Neisseria meningitidis 44/76-A

40.796

98.529

0.402

Multiple sequence alignment