Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AADG46_RS09840 Genome accession   NZ_AP028804
Coordinates   2176556..2178244 (+) Length   562 a.a.
NCBI ID   WP_148503289.1    Uniprot ID   -
Organism   Vibrio cholerae strain VN152     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2171556..2183244
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AADG46_RS09810 (VNVC001_18600) dsbC 2171705..2172457 (-) 753 WP_085603849.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  AADG46_RS09815 (VNVC001_18610) xerD 2172474..2173382 (-) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  AADG46_RS09820 (VNVC001_18620) fldB 2173520..2174038 (+) 519 WP_000690112.1 flavodoxin FldB -
  AADG46_RS09825 (VNVC001_18630) ampD 2174169..2174714 (-) 546 WP_340701785.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AADG46_RS09830 (VNVC001_18640) nadC 2175010..2175900 (+) 891 WP_114743264.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AADG46_RS09835 (VNVC001_18650) - 2176145..2176549 (+) 405 WP_000649343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AADG46_RS09840 (VNVC001_18660) pilB 2176556..2178244 (+) 1689 WP_148503289.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AADG46_RS09845 (VNVC001_18670) pilC 2178287..2179513 (+) 1227 WP_000648507.1 type II secretion system F family protein Machinery gene
  AADG46_RS09850 (VNVC001_18680) pilD 2179571..2180446 (+) 876 WP_000418753.1 A24 family peptidase Machinery gene
  AADG46_RS09855 (VNVC001_18690) coaE 2180443..2181051 (+) 609 WP_000011551.1 dephospho-CoA kinase -
  AADG46_RS09860 (VNVC001_18700) zapD 2181083..2181823 (+) 741 WP_000207194.1 cell division protein ZapD -
  AADG46_RS09865 (VNVC001_18710) yacG 2181981..2182178 (+) 198 WP_000162866.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62149.26 Da        Isoelectric Point: 6.2600

>NTDB_id=106917 AADG46_RS09840 WP_148503289.1 2176556..2178244(+) (pilB) [Vibrio cholerae strain VN152]
MLTNLVAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGFFHPRELTEQLSHIFGLPETDLSRYDYTPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAN
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQPNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=106917 AADG46_RS09840 WP_148503289.1 2176556..2178244(+) (pilB) [Vibrio cholerae strain VN152]
ATGCTCACCAACCTTGTTGCTATCTTGCGTCAGGCTGAATTAATCAGCGCGACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGGACGTCGGTGCCCGAGGCGCTGCTTGGTTTAGGTTTTTTTCACCCCCGAGAACTCACCGAACAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGATCTTAGCCGCTACGACTACACCCCCCTATGCCAACAGCTTGGGCTACGTGAA
CTGATTACCCGCTACGATGCCTTGCCTATTGCCAAACAAGGCAATTTACTGCTGCTCGCGGTCTCTGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCCACAGGATTACAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCAATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAACTCGCCAAT
CTGGTTAAAGTCAGTGATGATGAGCTGCAATCGATTGAAGATCTCAGCCAAGATGACTCTCCGGTTAGCCGCTTTATCAA
CCAAGTACTGCTCGATGCGGTGCGCAAAGGCGCCTCCGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGCATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCCGAGCGCCGCTTGCCTCAAGACGGGCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGAGAAAAAATCGTGCTGCGCCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCGCAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATT
TTAATGACCGGCCCCACCGGCAGCGGCAAAACCGTTTCGCTCTATACTGGGCTGCGCATTCTCAACACGTCACAGATCAA
TATCTCCACCGCGGAAGACCCAGTAGAAATAAACCTCTCTGGGATTAACCAAGTGCAAGTGCAACCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTACGCCAAGATCCGGATGTGGTGATGGTCGGTGAAATACGCGATCTGGAAACC
GCCGAAATCGCGGTCAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTCGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACATTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCCCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAATGCACGGGCGGCTATTCAGGGCGAGTTGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCGGAGGCCATTATGCAACGCGCCAGCATTCATCAAATTGAACGTTTAGCTAAAGCCAATG
GTATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGTTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.754

100

0.988
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

52.838

90.925

0.48
  pilB Acinetobacter baumannii D1279779

50.192

92.527

0.464
  pilB Acinetobacter baylyi ADP1

50.192

92.527

0.464
  pilF Neisseria gonorrhoeae MS11

45.73

100

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37.252

100

0.4
  pilF Thermus thermophilus HB27

39.372

96.263

0.379

Multiple sequence alignment