Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACAM55_RS04505 Genome accession   NZ_AP028664
Coordinates   926580..927809 (-) Length   409 a.a.
NCBI ID   WP_369654863.1    Uniprot ID   -
Organism   Variovorax sp. V213     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 921580..932809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM55_RS04480 (GmRootV213_08780) zapD 921613..922368 (-) 756 WP_369654859.1 cell division protein ZapD -
  ACAM55_RS04485 (GmRootV213_08790) - 922405..923421 (-) 1017 WP_369654860.1 hypothetical protein -
  ACAM55_RS04490 (GmRootV213_08800) - 923428..924921 (-) 1494 WP_369654861.1 MBOAT family protein -
  ACAM55_RS04495 (GmRootV213_08810) coaE 924999..925604 (-) 606 WP_369656334.1 dephospho-CoA kinase -
  ACAM55_RS04500 (GmRootV213_08820) - 925615..926580 (-) 966 WP_369654862.1 A24 family peptidase -
  ACAM55_RS04505 (GmRootV213_08830) pilC 926580..927809 (-) 1230 WP_369654863.1 type II secretion system F family protein Machinery gene
  ACAM55_RS04510 (GmRootV213_08840) pilB 927840..929579 (-) 1740 WP_369654864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM55_RS04520 (GmRootV213_08850) - 929822..930814 (-) 993 WP_369654865.1 polyprenyl synthetase family protein -
  ACAM55_RS04525 (GmRootV213_08860) rplU 931004..931315 (+) 312 WP_028259151.1 50S ribosomal protein L21 -
  ACAM55_RS04530 (GmRootV213_08870) rpmA 931328..931588 (+) 261 WP_369654866.1 50S ribosomal protein L27 -
  ACAM55_RS04535 (GmRootV213_08880) cgtA 931659..932735 (+) 1077 WP_369654867.1 Obg family GTPase CgtA -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44667.27 Da        Isoelectric Point: 9.6463

>NTDB_id=106771 ACAM55_RS04505 WP_369654863.1 926580..927809(-) (pilC) [Variovorax sp. V213]
MATVASTRTSSTLKEFVYEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKAIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANASVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMAMSEFFVSYWWLIFGVIGGGT
YFFLQAWKRNERVQRVMDRALLRVPIFGTLIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNTVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGTIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=106771 ACAM55_RS04505 WP_369654863.1 926580..927809(-) (pilC) [Variovorax sp. V213]
ATGGCAACAGTGGCATCCACCCGCACCTCAAGCACGCTCAAGGAATTCGTCTACGAGTGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTGCGCGGCGAGCTTCGGGCCGCCGGCGAGAACCAGGTTCAGGCCGCCCTGCGGCGCCAGGGGGTTCTCGCAT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGGCGATCAAGCCCAAGGACATTGCGATCTTCACGCGCCAGCTGGCA
ACGATGATGAAGGCCGGCGTGCCGCTTTTGCAGTCGTTCGACATCGTCGGCCGGGGCAATGCCAACGCAAGCGTTGCCAA
GCTGCTCAACGACATCCGCAGCGACGTGGAGACCGGGACCTCGCTTTCAGCCGCCTTCCGCAAGTTTCCGAAGTATTTCG
ACAACCTCTACTGCAACCTGGTGGAAGCCGGCGAAGCGGCCGGTATCCTGGAAGACCTGCTGGACCGCCTGGCCACCTAC
ATGGAAAAGACCGAGGCGATCAAGTCCAAGATCAAGTCGGCGCTGATGTACCCGACGTCGGTGGTCGTGGTCGCGTTCGT
GGTGGTCGCCATCATCATGATCTTCGTGATTCCGGCGTTCAAGCAGGTGTTCACCTCTTTCGGCGCCGATCTGCCCGCCC
CGACGCTGTTCGTGATGGCCATGAGCGAATTCTTCGTCTCCTACTGGTGGCTGATCTTCGGCGTGATCGGCGGAGGCACT
TACTTTTTCCTGCAGGCCTGGAAACGCAACGAACGCGTGCAGCGGGTCATGGACCGCGCGCTGCTGCGCGTGCCGATCTT
CGGCACGCTGATCGAAAAGTCGTGCGTGGCCCGATGGACCCGCACCCTTGCCACCATGTTCGCCGCCGGCGTTCCGCTGG
TCGAAGCGCTCGACTCGGTGGGCGGTGCGTCGGGCAACACGGTGTACGGCGACGCCACGGCCAAGATCCAGCAAGAGGTT
TCGACCGGCACCAGCCTCACCACGGCCATGACCAACGTCAACCTGTTCCCGTCGATGGTGATCCAGATGACGGCCATCGG
CGAAGAATCCGGCTCCATCGACCACATGCTCGGCAAGGCGGCGGACTTCTACGAATCCGAAGTGGACGACATGGTCGCGG
GCCTCTCGAGCCTGATGGAACCCATCATCATCGTGTTCCTCGGCACGATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTGGGCCAAGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.25

97.8

0.521
  pilG Neisseria gonorrhoeae MS11

52.273

96.822

0.506
  pilG Neisseria meningitidis 44/76-A

52.02

96.822

0.504
  pilC Acinetobacter baylyi ADP1

51.508

97.311

0.501
  pilC Legionella pneumophila strain ERS1305867

49.495

96.822

0.479
  pilC Acinetobacter baumannii D1279779

49.118

97.066

0.477
  pilC Vibrio cholerae strain A1552

40.931

99.756

0.408
  pilC Thermus thermophilus HB27

38.425

100

0.394
  pilC Vibrio campbellii strain DS40M4

39.651

98.044

0.389

Multiple sequence alignment