Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM55_RS04510 Genome accession   NZ_AP028664
Coordinates   927840..929579 (-) Length   579 a.a.
NCBI ID   WP_369654864.1    Uniprot ID   -
Organism   Variovorax sp. V213     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 922840..934579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM55_RS04490 (GmRootV213_08800) - 923428..924921 (-) 1494 WP_369654861.1 MBOAT family protein -
  ACAM55_RS04495 (GmRootV213_08810) coaE 924999..925604 (-) 606 WP_369656334.1 dephospho-CoA kinase -
  ACAM55_RS04500 (GmRootV213_08820) - 925615..926580 (-) 966 WP_369654862.1 A24 family peptidase -
  ACAM55_RS04505 (GmRootV213_08830) pilC 926580..927809 (-) 1230 WP_369654863.1 type II secretion system F family protein Machinery gene
  ACAM55_RS04510 (GmRootV213_08840) pilB 927840..929579 (-) 1740 WP_369654864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM55_RS04520 (GmRootV213_08850) - 929822..930814 (-) 993 WP_369654865.1 polyprenyl synthetase family protein -
  ACAM55_RS04525 (GmRootV213_08860) rplU 931004..931315 (+) 312 WP_028259151.1 50S ribosomal protein L21 -
  ACAM55_RS04530 (GmRootV213_08870) rpmA 931328..931588 (+) 261 WP_369654866.1 50S ribosomal protein L27 -
  ACAM55_RS04535 (GmRootV213_08880) cgtA 931659..932735 (+) 1077 WP_369654867.1 Obg family GTPase CgtA -
  ACAM55_RS04540 (GmRootV213_08890) proB 932821..933960 (+) 1140 WP_369654868.1 glutamate 5-kinase -
  ACAM55_RS04545 (GmRootV213_08900) - 933996..934529 (-) 534 WP_369654869.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 62721.98 Da        Isoelectric Point: 5.9515

>NTDB_id=106772 ACAM55_RS04510 WP_369654864.1 927840..929579(-) (pilB) [Variovorax sp. V213]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLSSRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLVDPKLCLAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSASETL
NNIVGGGDFEFDDVMADTSGDANEQAAVAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASP
PTLIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEK
ERLLNAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQD
PDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHLCRAPADV
PRQALLDAGFKDAELDGSWKPYRPVGCSVCNGGYKGRVGIYQVMPISEAIQAIILRDGSALDIARQSEAEGVRSLRQSGL
RKVMQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=106772 ACAM55_RS04510 WP_369654864.1 927840..929579(-) (pilB) [Variovorax sp. V213]
ATGGCTGCCGCCGAACTTCCTGTTAAAGAAAACACGCAAATCGCCCTCCCGGGCCTTGCGCGCGCCTTGGTTTCAGCCGG
CAAGCTTCCTGCGAAGACTGCGGAAGACATCTACCAGAAGTCGCTGAGCAGTCGCACCAGCTTCATTGCCGAGCTGACCG
GCAGCGGCGCCGTATCGGCCGCAGACCTGGCCCACACGCTTTCTTCCGCTTTCGGCGCCCCGCTGCTCGACCTGGACGCC
ATCGACCACCAGCGCCTGCCCAAGGACCTGGTGGACCCGAAGCTGTGCCTCGCCTACCGCATCGTGGTGCTCAGCAAGCG
CAACAACCGTCTCATTGTTGCAACAGCGGACCCCTCGGACCAGCAGGCCGTGGAAAAGATCAAGTTTGCGTCCCAGATGG
GCGTGGACTGGGTCATCGCCGAATACGACAAGCTGTCGCGCATGATCGAGGCCGCCGCTGTCAGCGCGTCGGAAACCCTC
AACAACATCGTCGGCGGGGGGGACTTCGAGTTCGACGATGTCATGGCCGATACGTCGGGCGACGCCAACGAACAGGCGGC
CGTCGCCGAAGTCGAGGATGCCCCGGTCGTCCGGTTCCTGCACAAAATGCTGCTCGACGGCGTCAGCATGCGGGCCTCGG
ACATTCACTTCGAGCCCTACGAGCACAACTACCGCGTGCGCTTTCGCATCGACGGCGAACTGCGCGAAATCGCGAGCCCG
CCCACGCTGATCAAGGACAAGCTGGCTTCGCGGATCAAGGTCATTTCGCGGCTCGACATCTCTGAAAAACGCGTGCCGCA
GGACGGCCGCATGAAGCTCAAGATCGGCCCGGATCGCGTGATCGACTTTCGCGTGAGCACGCTGCCCACGCTGTTCGGCG
AAAAGATCGTGATCCGTATTCTCGACCCGAGCAGTGCACGCCTGGGCATCGATGCGCTCGGCTACGACGCCGATGAAAAG
GAGCGGTTGCTGAATGCGATCGGGCGTCCCTACGGCATGGTGCTGGTGACGGGCCCCACGGGTTCCGGCAAGACGGTGTC
GCTTTACACCTGCCTGAACCTGCTGAACCAGCCGGGCGTCAATATCGCCACGGCCGAAGACCCGTCCGAAATCAACCTCC
CCGGCGTGAACCAGGTCAACGTGAACGAGCGTGCCGGACTCACGTTCGCCGCCGCGCTGCGCGCTTTCCTCCGGCAGGAT
CCCGACATCATCATGGTCGGCGAAATCCGGGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACGGGCCACCT
GGTGCTTTCGACGCTGCACACCAATGACGCGCCGACCACGCTCACGCGCATGCGGAACATGGGCATTGCGCCGTTCAACA
TCGCCTCGAGTGTCATCCTGATCACGGCGCAGCGGCTGGCCCGCCGCCTGTGCCATTTGTGCCGCGCGCCGGCCGACGTG
CCGCGCCAGGCTCTGCTCGACGCGGGCTTCAAGGACGCTGAACTCGATGGTTCCTGGAAGCCCTATCGTCCGGTCGGCTG
CTCCGTGTGCAATGGTGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCCGAAGCGATCCAGGCCATCA
TCCTGCGCGACGGCAGCGCGCTGGACATTGCGCGCCAGTCCGAAGCCGAGGGCGTTCGCTCGTTGCGGCAGTCCGGCCTC
AGAAAAGTCATGCAAGGGCTCACCTCACTCGAAGAAGTGCTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.196

98.791

0.535
  pilB Acinetobacter baylyi ADP1

53.076

98.273

0.522
  pilF Neisseria gonorrhoeae MS11

52.473

97.755

0.513
  pilB Legionella pneumophila strain ERS1305867

49.558

97.582

0.484
  pilB Vibrio cholerae strain A1552

46.316

98.446

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

45.614

98.446

0.449
  pilB Vibrio campbellii strain DS40M4

45.115

97.237

0.439
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.187

93.092

0.383
  pilF Thermus thermophilus HB27

37.368

98.446

0.368

Multiple sequence alignment