Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACAM52_RS20845 Genome accession   NZ_AP028663
Coordinates   4472584..4473813 (+) Length   409 a.a.
NCBI ID   WP_095949374.1    Uniprot ID   -
Organism   Variovorax sp. V118     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4467584..4478813
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM52_RS20815 (GmRootV118_41290) cgtA 4467671..4468747 (-) 1077 WP_062474512.1 Obg family GTPase CgtA -
  ACAM52_RS20820 (GmRootV118_41300) rpmA 4468820..4469080 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  ACAM52_RS20825 (GmRootV118_41310) rplU 4469093..4469404 (-) 312 WP_062474515.1 50S ribosomal protein L21 -
  ACAM52_RS20830 (GmRootV118_41320) - 4469587..4470579 (+) 993 WP_070063612.1 polyprenyl synthetase family protein -
  ACAM52_RS20840 (GmRootV118_41330) pilB 4470824..4472557 (+) 1734 WP_095949373.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM52_RS20845 (GmRootV118_41340) pilC 4472584..4473813 (+) 1230 WP_095949374.1 type II secretion system F family protein Machinery gene
  ACAM52_RS20850 (GmRootV118_41350) - 4473813..4474778 (+) 966 WP_369644498.1 A24 family peptidase -
  ACAM52_RS20855 (GmRootV118_41360) coaE 4474792..4475403 (+) 612 WP_369644499.1 dephospho-CoA kinase -
  ACAM52_RS20860 (GmRootV118_41370) - 4475480..4476973 (+) 1494 WP_369644500.1 MBOAT family protein -
  ACAM52_RS20865 (GmRootV118_41380) - 4476980..4478011 (+) 1032 WP_062474527.1 hypothetical protein -
  ACAM52_RS20870 (GmRootV118_41390) zapD 4478048..4478803 (+) 756 WP_070063618.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44733.44 Da        Isoelectric Point: 9.5343

>NTDB_id=106753 ACAM52_RS20845 WP_095949374.1 4472584..4473813(+) (pilC) [Variovorax sp. V118]
MATVASSRSQVTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKAIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANPSVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKEVFTSFGADLPAPTLFVMAISEFFVSYWWLIFGVLGGGI
YFFLQAWKRNERVQKVMDRLLLRIPVFGTLIEKSCIARWTRTLATMFAAGVPLVEALDSVGGASGNSVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=106753 ACAM52_RS20845 WP_095949374.1 4472584..4473813(+) (pilC) [Variovorax sp. V118]
ATGGCAACAGTGGCATCCTCCCGCTCCCAGGTCACGCACAAGGAATTCGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTGCGCGGCGAACTTCGCGCCGCCGGGGAGAACCAGGTGCAGGCGGCCTTGCGTCGCCAGGGCGTGCTGGCCT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGGCGATCAAGCCCAAGGACATCGCGATCTTCACGCGCCAGCTCGCG
ACCATGATGAAGGCGGGCGTGCCGCTGCTGCAGTCCTTCGACATCGTCGGCCGCGGCAACGCGAACCCGAGCGTCGCCAA
GCTGCTGAACGACATCCGCAGCGACGTCGAGACCGGCACCTCACTGTCGGCCGCTTTTCGCAAGTTTCCGAAATACTTCG
ACAACCTCTACTGCAACCTGGTGGAGGCCGGTGAGGCGGCCGGTATCCTGGAAGACCTGCTCGACCGCCTGGCCACCTAC
ATGGAGAAGACCGAGGCGATCAAATCCAAGATCAAGTCGGCATTGATGTACCCGACCTCGGTGGTCGTCGTGGCCTTCGT
GGTGGTGGCGATCATCATGATCTTCGTGATCCCCGCGTTCAAGGAGGTGTTCACCTCGTTCGGCGCCGACCTGCCTGCGC
CGACGTTATTCGTCATGGCGATCAGCGAATTTTTTGTTTCCTATTGGTGGCTGATCTTCGGCGTGCTCGGCGGTGGCATT
TACTTCTTTCTGCAAGCGTGGAAACGCAATGAGCGCGTCCAGAAAGTCATGGACCGCCTGCTGCTGCGCATTCCCGTCTT
CGGCACGCTGATCGAGAAATCCTGCATCGCCCGCTGGACCCGCACGCTCGCCACCATGTTCGCGGCCGGCGTGCCGCTGG
TGGAGGCGCTCGACTCGGTGGGCGGCGCATCGGGCAACTCGGTGTACGGCGACGCCACCGCCAAGATCCAGCAGGAGGTC
TCGACCGGCACCAGCCTCACGACGGCCATGACCAACGTCAACCTGTTCCCCTCGATGGTGATTCAAATGACCGCCATCGG
CGAGGAATCAGGCTCGATCGACCACATGCTCGGCAAGGCCGCCGACTTCTACGAGTCCGAAGTCGACGACATGGTCGCCG
GCCTCTCCAGCCTGATGGAACCCATCATCATCGTGTTCCTGGGCGTCATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.713

98.778

0.531
  pilG Neisseria gonorrhoeae MS11

52.897

97.066

0.513
  pilC Acinetobacter baylyi ADP1

50.852

100

0.511
  pilG Neisseria meningitidis 44/76-A

52.645

97.066

0.511
  pilC Legionella pneumophila strain ERS1305867

50

97.311

0.487
  pilC Acinetobacter baumannii D1279779

49.874

97.066

0.484
  pilC Vibrio cholerae strain A1552

40.955

97.311

0.399
  pilC Thermus thermophilus HB27

38.425

100

0.394
  pilC Vibrio campbellii strain DS40M4

39.055

98.289

0.384

Multiple sequence alignment