Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM52_RS20840 Genome accession   NZ_AP028663
Coordinates   4470824..4472557 (+) Length   577 a.a.
NCBI ID   WP_095949373.1    Uniprot ID   -
Organism   Variovorax sp. V118     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4465824..4477557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM52_RS20810 (GmRootV118_41280) proB 4466451..4467590 (-) 1140 WP_369644497.1 glutamate 5-kinase -
  ACAM52_RS20815 (GmRootV118_41290) cgtA 4467671..4468747 (-) 1077 WP_062474512.1 Obg family GTPase CgtA -
  ACAM52_RS20820 (GmRootV118_41300) rpmA 4468820..4469080 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  ACAM52_RS20825 (GmRootV118_41310) rplU 4469093..4469404 (-) 312 WP_062474515.1 50S ribosomal protein L21 -
  ACAM52_RS20830 (GmRootV118_41320) - 4469587..4470579 (+) 993 WP_070063612.1 polyprenyl synthetase family protein -
  ACAM52_RS20840 (GmRootV118_41330) pilB 4470824..4472557 (+) 1734 WP_095949373.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM52_RS20845 (GmRootV118_41340) pilC 4472584..4473813 (+) 1230 WP_095949374.1 type II secretion system F family protein Machinery gene
  ACAM52_RS20850 (GmRootV118_41350) - 4473813..4474778 (+) 966 WP_369644498.1 A24 family peptidase -
  ACAM52_RS20855 (GmRootV118_41360) coaE 4474792..4475403 (+) 612 WP_369644499.1 dephospho-CoA kinase -
  ACAM52_RS20860 (GmRootV118_41370) - 4475480..4476973 (+) 1494 WP_369644500.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62568.83 Da        Isoelectric Point: 5.7128

>NTDB_id=106752 ACAM52_RS20840 WP_095949373.1 4470824..4472557(+) (pilB) [Variovorax sp. V118]
MAAAEIPVKETTQIALPGLARALVSAGKLAAKTAEDIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAASAAETI
NSIVGTEFEFDDVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMLCRAPIDIPR
EALLDAGFQEEDINGTWKPYRPVGCAACSGGYKGRVGIYQVMPISEAIQEIILRDGSALDIANQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=106752 ACAM52_RS20840 WP_095949373.1 4470824..4472557(+) (pilB) [Variovorax sp. V118]
ATGGCCGCTGCCGAAATTCCCGTTAAAGAAACTACGCAGATCGCCCTTCCCGGCCTGGCGCGCGCCCTCGTGTCGGCTGG
CAAGCTCGCGGCGAAGACAGCCGAAGACATCTATCAGAAGTCACTGAGCGGCCGCACCAGCTTCATCGCCGAACTGACCG
GCACCGGTGCCGTCTCGGCTGCGGACCTCGCGCACACCCTGTCGAGCGCCTTCGGCGCCCCGCTGCTCGACCTCGATGCC
ATCGATCACCAGCGCCTGCCCAAGGACCTGCTCGATCCGAAGCTCTGCCATGCGTACCGCATCGTCGTTCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCGACGGCCGACCCGTCGGACCAGCAGGCCGCGGAAAAAATCAAGTTCGCGTCGCAGATGG
GCGTGGACTGGGTCATCGCCGAGTACGACAAGCTCTCGCGCATGATCGAAGCCGCTGCAGCGAGCGCGGCTGAAACCATC
AACAGCATCGTCGGCACCGAGTTCGAGTTCGACGACGTCACGGCCGACACCTCGGGCGATGCCAACGAGCAGGCCATCGC
CGAAGTCGAAGACGCCCCTGTGGTGCGCTTCCTGCACAAGATGCTGCTCGACGCAGTCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCCTACGAACACCATTACCGGGTGCGCTTCCGGGTCGACGGCGAGCTGCGCGAAATCGCCAGCCCACCCACG
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATTTCGCGGCTCGACATCTCGGAAAAACGCGTGCCGCAAGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTCCGTGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTCGTGCGTATCCTGGACCCGAGCAGCGCGCGCATGGGCATCGACGCCCTGGGCTACGACGCCGACGAGAAAGAACGA
CTCCTCCACGCCATCGGCCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACGGGTTCAGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAACCTGCTCAACCAGCCGGGCGTCAACATCGCGACGGCGGAAGACCCGTCGGAAATCAACCTGCCTGGGG
TCAATCAGGTCAACGTCAACGAGAAGGCCGGCCTCACCTTCGCGGCCGCGCTGCGTGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGATATCTCGATCAAGGCCGCGCAAACCGGTCACTTGGTGCT
GTCGACGCTGCACACCAACGACGCCCCGACCACACTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACATCGCGT
CGAGCGTGATCCTGATCACCGCGCAACGCCTCGCGCGCCGGCTGTGCATGCTGTGCCGCGCGCCGATCGACATCCCGCGC
GAAGCCCTGCTCGATGCCGGCTTCCAAGAGGAAGACATCAACGGCACCTGGAAGCCCTACCGCCCGGTCGGCTGCGCCGC
CTGCAGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATTTCGGAAGCCATCCAGGAAATCATCCTGC
GCGACGGCAGTGCGCTGGACATCGCGAACCAATCCGAAGCCGAAGGCGTGCGCTCGCTGCGCCAGTCCGGCCTTCGCAAA
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTCGTCGCCTGCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.31

99.48

0.53
  pilB Acinetobacter baumannii D1279779

54.107

97.054

0.525
  pilF Neisseria gonorrhoeae MS11

52.669

97.4

0.513
  pilB Legionella pneumophila strain ERS1305867

48.319

97.92

0.473
  pilB Vibrio cholerae strain A1552

46.607

97.054

0.452
  pilB Vibrio campbellii strain DS40M4

45.826

97.574

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.455

97.227

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.254

97.574

0.383
  pilF Thermus thermophilus HB27

37.786

98.614

0.373

Multiple sequence alignment