Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACAM58_RS20720 Genome accession   NZ_AP028661
Coordinates   4359381..4360610 (+) Length   409 a.a.
NCBI ID   WP_015866995.1    Uniprot ID   A0AAW8EJE7
Organism   Variovorax sp. V116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4354381..4365610
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM58_RS20690 (GmRootV116_41280) cgtA 4354457..4355545 (-) 1089 WP_015866990.1 Obg family GTPase CgtA -
  ACAM58_RS20695 (GmRootV116_41290) rpmA 4355616..4355873 (-) 258 WP_015866991.1 50S ribosomal protein L27 -
  ACAM58_RS20700 (GmRootV116_41300) rplU 4355886..4356197 (-) 312 WP_015866992.1 50S ribosomal protein L21 -
  ACAM58_RS20705 (GmRootV116_41310) - 4356386..4357378 (+) 993 WP_021008896.1 polyprenyl synthetase family protein -
  ACAM58_RS20715 (GmRootV116_41320) pilB 4357618..4359351 (+) 1734 WP_015866994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM58_RS20720 (GmRootV116_41330) pilC 4359381..4360610 (+) 1230 WP_015866995.1 type II secretion system F family protein Machinery gene
  ACAM58_RS20725 (GmRootV116_41340) - 4360610..4361575 (+) 966 WP_369663452.1 A24 family peptidase -
  ACAM58_RS20730 (GmRootV116_41350) coaE 4361586..4362191 (+) 606 WP_369663453.1 dephospho-CoA kinase -
  ACAM58_RS20735 (GmRootV116_41360) - 4362270..4363763 (+) 1494 WP_369663454.1 MBOAT family protein -
  ACAM58_RS20740 (GmRootV116_41370) - 4363770..4364789 (+) 1020 WP_015866999.1 hypothetical protein -
  ACAM58_RS20745 (GmRootV116_41380) zapD 4364826..4365581 (+) 756 WP_015867000.1 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44736.33 Da        Isoelectric Point: 9.6463

>NTDB_id=106740 ACAM58_RS20720 WP_015866995.1 4359381..4360610(+) (pilC) [Variovorax sp. V116]
MATVASTRTSNTLKEFVYEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKSIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANPSVAKLLNDIRSDVETGTSLSSAFRKFPKYFDNLYCNLVEAGEAAGILEDLLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMAMSEFFVSYWWLIFGVVGGGT
YFFLQAWKRNERVQRVMDRALLRVPIFGTLIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNTVYGDATAKIQQEV
STGTSLTTAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=106740 ACAM58_RS20720 WP_015866995.1 4359381..4360610(+) (pilC) [Variovorax sp. V116]
ATGGCAACAGTGGCATCCACCCGCACCTCGAACACGCTCAAGGAATTTGTCTACGAGTGGGAGGGCAAGGACCGCAACGG
CAAGCTGGTGCGCGGCGAGCTCCGGGCCGCCGGCGAGAACCAGGTGCAGGCCGCCCTGCGGCGCCAGGGCGTCCTGGCGT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGAGCATCAAGCCCAAGGACATCGCGATCTTCACGCGCCAGCTGGCA
ACCATGATGAAGGCCGGCGTGCCGCTGCTGCAGTCCTTCGACATCGTGGGCCGCGGCAATGCGAACCCGAGCGTGGCCAA
GCTGCTCAACGACATCCGCAGCGACGTGGAAACCGGCACCTCGCTGTCGTCCGCCTTCCGCAAGTTTCCGAAGTACTTCG
ACAACCTCTACTGCAACCTGGTGGAAGCCGGCGAAGCGGCCGGTATCCTGGAAGACCTGCTGGACCGCCTGGCCACCTAC
ATGGAAAAGACCGAGGCGATCAAGTCGAAGATCAAGTCGGCGCTGATGTATCCCACCTCCGTGGTGGTGGTCGCGTTCGT
GGTGGTGGCCATCATCATGATCTTCGTGATTCCGGCCTTCAAGCAGGTGTTCACCTCGTTCGGCGCCGACCTGCCCGCGC
CCACGCTGTTCGTGATGGCCATGAGCGAATTCTTCGTCTCCTACTGGTGGCTGATCTTCGGCGTGGTCGGCGGCGGCACC
TACTTCTTCCTGCAGGCCTGGAAGCGCAACGAACGCGTGCAGCGGGTCATGGACCGCGCGCTGCTGCGCGTGCCGATCTT
CGGTACGCTGATCGAGAAGTCGTGCGTGGCCCGCTGGACCCGCACCCTTGCGACCATGTTCGCGGCCGGCGTTCCGCTGG
TCGAGGCGCTCGACTCGGTGGGCGGCGCCTCGGGCAACACCGTCTACGGCGACGCCACGGCCAAGATCCAGCAGGAAGTC
TCGACCGGCACCAGCCTCACGACGGCCATGACCAACGTCAACCTGTTCCCCTCGATGGTGATCCAGATGACGGCCATCGG
CGAAGAATCCGGCTCGATCGACCACATGCTCGGCAAGGCCGCCGACTTCTACGAGTCCGAGGTGGACGACATGGTGGCCG
GCCTCTCGAGCCTGATGGAGCCCATCATCATCGTGTTCCTGGGCGTGATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCGATCTTCAAGTTGGGCCAGGTCGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54

97.8

0.528
  pilG Neisseria gonorrhoeae MS11

52.525

96.822

0.509
  pilG Neisseria meningitidis 44/76-A

52.273

96.822

0.506
  pilC Acinetobacter baylyi ADP1

51.508

97.311

0.501
  pilC Acinetobacter baumannii D1279779

49.622

97.066

0.482
  pilC Legionella pneumophila strain ERS1305867

48.883

98.533

0.482
  pilC Vibrio cholerae strain A1552

40.441

99.756

0.403
  pilC Thermus thermophilus HB27

38.663

100

0.396
  pilC Vibrio campbellii strain DS40M4

39.651

98.044

0.389

Multiple sequence alignment