Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACAM58_RS20715 Genome accession   NZ_AP028661
Coordinates   4357618..4359351 (+) Length   577 a.a.
NCBI ID   WP_015866994.1    Uniprot ID   -
Organism   Variovorax sp. V116     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4352618..4364351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM58_RS20680 (GmRootV116_41260) - 4352683..4353213 (+) 531 WP_015866988.1 CNP1-like family protein -
  ACAM58_RS20685 (GmRootV116_41270) proB 4353279..4354418 (-) 1140 WP_015866989.1 glutamate 5-kinase -
  ACAM58_RS20690 (GmRootV116_41280) cgtA 4354457..4355545 (-) 1089 WP_015866990.1 Obg family GTPase CgtA -
  ACAM58_RS20695 (GmRootV116_41290) rpmA 4355616..4355873 (-) 258 WP_015866991.1 50S ribosomal protein L27 -
  ACAM58_RS20700 (GmRootV116_41300) rplU 4355886..4356197 (-) 312 WP_015866992.1 50S ribosomal protein L21 -
  ACAM58_RS20705 (GmRootV116_41310) - 4356386..4357378 (+) 993 WP_021008896.1 polyprenyl synthetase family protein -
  ACAM58_RS20715 (GmRootV116_41320) pilB 4357618..4359351 (+) 1734 WP_015866994.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACAM58_RS20720 (GmRootV116_41330) pilC 4359381..4360610 (+) 1230 WP_015866995.1 type II secretion system F family protein Machinery gene
  ACAM58_RS20725 (GmRootV116_41340) - 4360610..4361575 (+) 966 WP_369663452.1 A24 family peptidase -
  ACAM58_RS20730 (GmRootV116_41350) coaE 4361586..4362191 (+) 606 WP_369663453.1 dephospho-CoA kinase -
  ACAM58_RS20735 (GmRootV116_41360) - 4362270..4363763 (+) 1494 WP_369663454.1 MBOAT family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62668.92 Da        Isoelectric Point: 6.2073

>NTDB_id=106739 ACAM58_RS20715 WP_015866994.1 4357618..4359351(+) (pilB) [Variovorax sp. V116]
MAAAELPVKENTQIALPGLARALVSAGKLPAKTAEDIYQKSLSGRTSFIAELTGSGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAVEKIKFASQMGVDWVIAEYDKLSRMIEAAAVTAAETI
NSIVGAEFEFDDVSADTSGDANEQAIAEVEDAPVVRFLHKMLLDGVSMRASDIHFEPYEHNYRVRFRIDGELREIASPPT
LIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCHVCRAPADMPR
QALLDAGFKEEQLDGTWKPYRPVGCAACSNGYKGRVGIYQVMPISEAIQAIILRDGSALDIARQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=106739 ACAM58_RS20715 WP_015866994.1 4357618..4359351(+) (pilB) [Variovorax sp. V116]
ATGGCTGCTGCCGAACTTCCTGTTAAAGAAAACACGCAAATCGCCCTCCCGGGTCTTGCGCGTGCCTTGGTCTCGGCCGG
CAAGCTTCCTGCGAAGACCGCGGAAGACATCTATCAAAAGTCGCTGAGCGGCCGCACCAGCTTCATTGCCGAGCTGACCG
GCAGCGGCGCCGTCTCGGCCGCCGACCTCGCCCACACGCTGTCCAGCGCCTTCGGCGCGCCGCTGCTGGACCTGGACGCC
ATCGACCACCAGCGCCTGCCCAAGGACCTGCTCGACCCGAAGCTGTGCCACGCCTACCGGATCGTGGTTCTCAGCAAGCG
CAACAACCGTCTCATTGTTGCAACGGCCGACCCTTCGGACCAGCAGGCGGTGGAAAAGATCAAGTTCGCCTCCCAGATGG
GCGTGGACTGGGTCATTGCCGAATACGACAAGCTCTCGCGCATGATCGAAGCCGCCGCGGTCACTGCGGCGGAAACCATC
AACAGCATCGTCGGCGCCGAGTTCGAGTTCGACGACGTCTCGGCGGACACCTCGGGCGATGCCAACGAACAGGCCATCGC
CGAGGTCGAGGACGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGACGGTGTCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCCTACGAGCACAACTACCGGGTGCGCTTTCGCATCGACGGCGAGTTGCGCGAGATCGCGAGCCCGCCCACG
CTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATCTCGCGGCTCGACATTTCGGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTGATCCGTATCCTGGACCCGAGCAGCGCGCGCCTGGGCATCGACGCACTGGGCTACGACGCCGACGAAAAGGAGCGC
CTGCTGCATGCGATCGGCCGCCCCTATGGCATGGTGCTGGTGACGGGCCCCACGGGTTCGGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCCGGCGTCAACATCGCCACGGCGGAAGACCCGTCCGAAATCAACCTGCCCGGCG
TGAACCAGGTGAACGTCAACGAGCGCGCCGGGCTCACCTTCGCGGCGGCGCTGCGCGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACGGGCCACCTGGTGCT
CTCGACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGCATGCGCAACATGGGCATTGCGCCGTTCAACATTGCCT
CCAGCGTGATCCTGATCACGGCGCAGCGGCTCGCGCGGCGCCTGTGCCACGTATGCCGCGCGCCGGCCGACATGCCGCGC
CAGGCGCTGCTGGATGCGGGCTTCAAGGAAGAACAGCTCGACGGTACCTGGAAGCCCTATCGGCCGGTCGGCTGCGCGGC
GTGCAGCAACGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATCTCGGAAGCCATCCAGGCGATCATCCTGC
GCGACGGCAGCGCACTCGACATCGCACGCCAGTCCGAGGCCGAGGGCGTGCGCTCGCTGCGGCAATCGGGCCTGCGAAAA
GTCATGCAAGGGCTCACCTCACTCGAAGAAGTGGTGGCGGTCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.697

98.44

0.529
  pilB Acinetobacter baylyi ADP1

53.654

97.227

0.522
  pilF Neisseria gonorrhoeae MS11

52.22

97.574

0.51
  pilB Legionella pneumophila strain ERS1305867

49.204

97.92

0.482
  pilB Vibrio cholerae strain A1552

46.964

97.054

0.456
  pilB Vibrio campbellii strain DS40M4

45.664

97.92

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.374

97.4

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.963

94.107

0.376
  pilF Thermus thermophilus HB27

37.478

98.96

0.371

Multiple sequence alignment