Detailed information NTG1066087

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: ACF8YQ_RS12030
• Genome accession: NZ_CP172297
• Coordinates: 2599504..2599974 (-)
• Length: 156 a.a.
• NCBI ID: WP_396621536.1
• Uniprot ID: -
• Organism: Marinobacter sp. W-8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2584717..2599435	2599504..2599974	flank	69

Gene organization within MGE regions

Location: 2584717..2599974

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACF8YQ_RS11955	-	2584717..2585328 (+)	612	WP_396621523.1	c-type cytochrome	-
ACF8YQ_RS11960	-	2585415..2586116 (-)	702	WP_396621524.1	Fic family protein	-
ACF8YQ_RS11965	-	2586494..2586793 (-)	300	WP_023011844.1	CcdB family protein	-
ACF8YQ_RS11970	-	2586793..2587038 (-)	246	WP_228223898.1	type II toxin-antitoxin system CcdA family antitoxin	-
ACF8YQ_RS11975	-	2587204..2587752 (+)	549	WP_396621525.1	hypothetical protein	-
ACF8YQ_RS11980	-	2588056..2588361 (-)	306	WP_396621526.1	hypothetical protein	-
ACF8YQ_RS11985	-	2588573..2589694 (+)	1122	WP_396621527.1	UDP-N-acetylglucosamine 2-epimerase	-
ACF8YQ_RS11990	-	2589744..2590187 (+)	444	WP_396621528.1	glycosyltransferase	-
ACF8YQ_RS11995	-	2590180..2591205 (+)	1026	WP_396621529.1	glycosyltransferase family 4 protein	-
ACF8YQ_RS12000	-	2591558..2592223 (+)	666	WP_396621530.1	class I SAM-dependent methyltransferase	-
ACF8YQ_RS12005	-	2592220..2593122 (+)	903	WP_396621531.1	glycosyltransferase family 2 protein	-
ACF8YQ_RS12010	-	2593213..2594499 (-)	1287	WP_396621532.1	lipopolysaccharide biosynthesis protein	-
ACF8YQ_RS12015	-	2594560..2595423 (-)	864	WP_396621533.1	glycosyltransferase family 2 protein	-
ACF8YQ_RS12020	-	2595468..2597471 (-)	2004	WP_396621534.1	tetratricopeptide repeat protein	-
ACF8YQ_RS12025	-	2597468..2599435 (-)	1968	WP_396621535.1	tetratricopeptide repeat protein	-
ACF8YQ_RS12030	pilE	2599504..2599974 (-)	471	WP_396621536.1	pilin	Machinery gene

Sequence

Protein

• Length: 156 a.a.
• Molecular weight: 16304.50 Da
• Isoelectric Point: 7.7727

>NTDB_id=1066087 ACF8YQ_RS12030 WP_396621536.1 2599504..2599974(-) (pilE) [Marinobacter sp. W-8]
MTTLEVDNMQKIQMNKGQQGFTLIELMIVVAIIGILAAVAIPAYQDYTIRSQVSEGMSLTSGVKTAVSEFVQTNGAYPSN
NANAGIATTITGNYVSSVTNASGVITVTYSNTGSQSANAQINGKTMVLSAFTSAGSVQWTCKAGTIEAKYLPSSCR

Nucleotide

• Length: 471 bp

>NTDB_id=1066087 ACF8YQ_RS12030 WP_396621536.1 2599504..2599974(-) (pilE) [Marinobacter sp. W-8]
ATGACAACTCTTGAGGTTGATAACATGCAAAAGATTCAGATGAACAAAGGTCAGCAGGGTTTTACTCTGATCGAACTGAT
GATCGTTGTGGCGATTATTGGTATTTTGGCGGCTGTGGCTATTCCGGCTTATCAGGATTATACGATTCGGTCTCAAGTCT
CTGAAGGGATGTCCTTGACAAGCGGTGTTAAGACAGCGGTTTCTGAATTTGTTCAGACAAATGGGGCTTATCCAAGCAAT
AACGCGAACGCCGGAATTGCAACAACCATTACAGGAAACTATGTCTCATCAGTGACGAATGCCAGCGGCGTTATTACTGT
GACATACAGCAACACTGGCTCACAGAGTGCTAACGCTCAGATCAACGGCAAGACAATGGTGCTTTCCGCTTTCACTTCCG
CTGGAAGTGTTCAGTGGACCTGTAAGGCGGGAACAATCGAAGCGAAGTATCTGCCTTCTAGCTGCCGCTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (16-40)

• Pilin (bacterial filament) (Pilin) (50-156)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Neisseria gonorrhoeae MS11	47.531	100	0.494
pilE	Neisseria gonorrhoeae strain FA1090	47.205	100	0.487
pilA2	Legionella pneumophila str. Paris	53.191	90.385	0.481
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	39.362	100	0.474
pilA2	Legionella pneumophila strain ERS1305867	52.482	90.385	0.474
pilA/pilA1	Eikenella corrodens VA1	43.671	100	0.442
pilA	Ralstonia pseudosolanacearum GMI1000	41.071	100	0.442
comP	Acinetobacter baylyi ADP1	43.709	96.795	0.423
pilA/pilAI	Pseudomonas stutzeri DSM 10701	41.333	96.154	0.397