Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACH105_RS15850 Genome accession   NZ_CP172234
Coordinates   3370898..3372601 (-) Length   567 a.a.
NCBI ID   WP_395763936.1    Uniprot ID   -
Organism   Stutzerimonas balearica strain Y11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3365898..3377601
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACH105_RS15815 (ACH105_15815) rpsI 3366007..3366399 (-) 393 WP_041104005.1 30S ribosomal protein S9 -
  ACH105_RS15820 (ACH105_15820) rplM 3366414..3366842 (-) 429 WP_041104003.1 50S ribosomal protein L13 -
  ACH105_RS15825 (ACH105_15825) - 3367209..3367919 (+) 711 WP_200290958.1 energy-coupling factor ABC transporter permease -
  ACH105_RS15830 (ACH105_15830) yacG 3367913..3368110 (-) 198 WP_074519929.1 DNA gyrase inhibitor YacG -
  ACH105_RS15835 (ACH105_15835) coaE 3368107..3368715 (-) 609 WP_312380414.1 dephospho-CoA kinase -
  ACH105_RS15840 (ACH105_15840) pilD 3368809..3369678 (-) 870 WP_395763935.1 prepilin peptidase Machinery gene
  ACH105_RS15845 (ACH105_15845) pilC 3369675..3370895 (-) 1221 WP_061340859.1 type II secretion system F family protein Machinery gene
  ACH105_RS15850 (ACH105_15850) pilB 3370898..3372601 (-) 1704 WP_395763936.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACH105_RS15855 (ACH105_15855) pilV 3372970..3373155 (+) 186 WP_272890074.1 type IV pilin protein Machinery gene
  ACH105_RS15860 (ACH105_15860) - 3373175..3373642 (+) 468 WP_145996470.1 hypothetical protein -
  ACH105_RS15865 (ACH105_15865) - 3374275..3374997 (+) 723 WP_395763937.1 hypothetical protein -
  ACH105_RS15870 (ACH105_15870) - 3374999..3375838 (+) 840 WP_395764862.1 hypothetical protein -
  ACH105_RS15875 (ACH105_15875) - 3375835..3376731 (+) 897 WP_395763938.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62185.13 Da        Isoelectric Point: 5.4649

>NTDB_id=1065786 ACH105_RS15850 WP_395763936.1 3370898..3372601(-) (pilB) [Stutzerimonas balearica strain Y11]
MNENAPLGGLARQLVLVGQLDEKAAQQAQQQALRNQVPLVSWLVQNKLVKGRVLVDIAGEQFGVPFFDLSALDKDSQPKD
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEAVLVEDDRLGEAIEKFFESATSSLDGLTDSELD
GLDIEVAGEAKEDDAAHNEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSSTKSVDFRVNTLPTLWGEKIVMRILDSASAKMGIDALGYEDDQKELYLSALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKAEVDVPREVLLKEGFP
EARIGTFKLYGPVGCDSCKGGYKGRVGIYEVVKITPSLQRLIMEEGNSIDIALQARKDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1065786 ACH105_RS15850 WP_395763936.1 3370898..3372601(-) (pilB) [Stutzerimonas balearica strain Y11]
ATGAACGAAAACGCACCTCTTGGTGGTTTGGCTCGCCAGCTTGTACTGGTAGGCCAGCTCGACGAAAAGGCGGCGCAGCA
GGCTCAGCAACAGGCCCTGCGCAACCAGGTGCCCCTGGTGAGTTGGTTGGTACAGAACAAGTTGGTCAAAGGGCGGGTGC
TCGTTGACATCGCCGGCGAGCAGTTCGGCGTGCCGTTCTTCGATCTGAGTGCGCTAGACAAGGATTCGCAGCCAAAGGAC
CTCGTCAGCGAAAAGCTGGTTCGCCAGCACCGCGCATTGCCGCTTCAGCGGCGCGGCAACAAGCTTTATGTCGCCGTGTC
GGATCCGACCAATCATCAGGCTATCAGCGATATCCAGTTCAACACCGGCCTCTCCGTCGAGGCCGTACTGGTTGAGGACG
ACAGGCTCGGCGAGGCGATCGAGAAGTTTTTCGAGTCGGCGACTTCCTCCCTCGATGGGTTGACCGATTCCGAGTTGGAC
GGCCTGGATATCGAGGTTGCGGGTGAGGCCAAGGAAGATGATGCAGCGCATAACGAGGCCGATGATGCGCCGGTGGTGAG
ATTCGTCAACAAGATGCTGCTGGATGCAATAAAGGGCGGCTCATCAGATTTGCACTTTGAACCTTATGAAAAGAGCTATC
GGGTTCGTTTTCGTACGGATGGCATGTTGCATGAGGTCGCCCGCCCACCAGTGCAGTTGGTCAGTCGCATTTCAGCACGG
TTGAAGGTGATGGCCAGTCTGGATATCGCCGAACGACGCAAGCCCCAGGATGGGCGCATCAAGATGAAGGTGTCGAGCAC
CAAATCCGTCGATTTCCGGGTGAATACGCTTCCGACCCTCTGGGGCGAAAAGATCGTCATGCGAATCCTCGACTCGGCGA
GTGCCAAGATGGGCATCGATGCGCTGGGCTACGAGGATGACCAGAAGGAGCTGTACCTGAGCGCGCTTAAGCAGCCGCAA
GGAATGATCCTCGTTACGGGGCCGACTGGTTCCGGCAAGACCGTTTCCCTTTACACCGGCCTGAACATCCTCAATACGGT
CGACGTGAACATCTCAACGGCCGAGGATCCGGTGGAAATCAACCTCGAGGGGATCAATCAGGTCAACGTTAACCCCAGGC
AGGGCATGGACTTCGCACAGGCCCTGCGTGCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGTGAGATTCGCGAT
CTGGAAACTGCCGAGATCGCGATCAAGGCGGCACAGACCGGCCACATGGTGATGTCGACGCTGCATACCAATAGTGCGGC
AGAGACCCTGACGCGGTTGCGCAACATGGGAGTGCCGGCTTTCAACATCGCGACCTCGGTGAACTTGATCATTGCTCAGC
GGCTGGCGCGAAAACTTTGCCCGAGCTGCAAGGCGGAAGTTGATGTGCCGCGTGAGGTATTGCTCAAGGAGGGCTTCCCG
GAAGCTCGGATAGGCACCTTCAAGCTCTACGGGCCGGTTGGTTGCGACAGTTGCAAGGGGGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCCTCGCTGCAGCGACTTATCATGGAAGAAGGAAATTCCATCGATATCGCACTTC
AGGCGCGCAAGGATGGCTTCAACGACCTGCGCACCTCTGGCTTGGTCAAGGCCATGAATGGCGTAACCAGTCTGGAAGAA
GTCAACCGCGTCACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.431

99.118

0.589
  pilB Acinetobacter baylyi ADP1

58.23

99.647

0.58
  pilB Legionella pneumophila strain ERS1305867

54.466

100

0.549
  pilB Vibrio cholerae strain A1552

51.099

96.296

0.492
  pilB Vibrio campbellii strain DS40M4

50.273

96.825

0.487
  pilF Neisseria gonorrhoeae MS11

53.62

90.123

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

53.635

89.771

0.481
  pilF Thermus thermophilus HB27

38.366

99.295

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40

94.356

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

40.385

91.711

0.37

Multiple sequence alignment