Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHWYG_RS05465 Genome accession   NZ_CP172082
Coordinates   1120448..1121839 (-) Length   463 a.a.
NCBI ID   WP_032823891.1    Uniprot ID   -
Organism   Haemophilus influenzae strain GA54827     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1115448..1126839
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHWYG_RS05430 - 1115697..1115903 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  ACHWYG_RS05435 - 1115977..1116183 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  ACHWYG_RS05440 cueR 1116260..1116646 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  ACHWYG_RS05445 metJ 1116660..1116977 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  ACHWYG_RS05450 rho 1117225..1118487 (+) 1263 WP_005666690.1 transcription termination factor Rho -
  ACHWYG_RS05455 pilD 1118542..1119234 (-) 693 WP_032823889.1 A24 family peptidase Machinery gene
  ACHWYG_RS05460 pilC 1119231..1120451 (-) 1221 WP_032823890.1 type II secretion system F family protein Machinery gene
  ACHWYG_RS05465 pilB 1120448..1121839 (-) 1392 WP_032823891.1 GspE/PulE family protein Machinery gene
  ACHWYG_RS05470 pilA 1121836..1122276 (-) 441 WP_032823893.1 prepilin peptidase-dependent pilin Machinery gene
  ACHWYG_RS05475 ampD 1122391..1122951 (+) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHWYG_RS05480 corC 1123574..1124473 (+) 900 WP_032823895.1 CNNM family magnesium/cobalt transport protein CorC -
  ACHWYG_RS05485 lnt 1124496..1126013 (+) 1518 WP_080279450.1 apolipoprotein N-acyltransferase -
  ACHWYG_RS05490 rsmE 1126063..1126800 (+) 738 WP_112103475.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52603.10 Da        Isoelectric Point: 5.6550

>NTDB_id=1065013 ACHWYG_RS05465 WP_032823891.1 1120448..1121839(-) (pilB) [Haemophilus influenzae strain GA54827]
MIDLTSVKPRVKAQNGEIFTISPDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPILL
ESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVLQ
PQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELGM
TESQQQAFQHALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINSQIGLDFNRLLRT
FLRQDPDIIMLGEIRDEESAIIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKCG
GNLINSCDCNQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=1065013 ACHWYG_RS05465 WP_032823891.1 1120448..1121839(-) (pilB) [Haemophilus influenzae strain GA54827]
ATGATCGACTTAACTAGTGTAAAACCTCGTGTAAAAGCACAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATGGGC
GCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGCTAG
GGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTGTTA
GAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGTGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTATCA
ACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCTTAC
AAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTACAA
CCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAGTGA
AAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCTTAC
CAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGCATG
ACTGAAAGCCAACAACAGGCATTTCAACACGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGGCCCAACAGGAAG
TGGAAAAAGTATCTCACTTTACACTGCACTTCAATGGCTAAATACACCTGATAAACACATTATGACAGCGGAAGATCCCA
TTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATTCACAGATTGGATTAGATTTTAACCGCCTATTGCGTACT
TTTTTACGCCAAGATCCCGATATCATTATGTTAGGCGAAATTCGCGATGAAGAAAGTGCAATAATTGCATTACGTGCCGC
ACAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGCTTACAACAACTTGGTA
TTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGCGGT
GGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCAGCA
GAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGAAAG
AAATTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(127-394)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(23-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.696

98.056

0.948
  pilB Haemophilus influenzae Rd KW20

96.026

97.84

0.94
  pilB Glaesserella parasuis strain SC1401

57.826

99.352

0.575
  pilB Vibrio cholerae strain A1552

40.574

100

0.428
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.417
  pilB Legionella pneumophila strain ERS1305867

38.742

100

0.413
  pilB Acinetobacter baylyi ADP1

37.903

100

0.406
  pilB Acinetobacter baumannii D1279779

38.351

100

0.402
  pilF Neisseria gonorrhoeae MS11

36.345

100

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.966

100

0.374
  pilF Thermus thermophilus HB27

35.391

100

0.371

Multiple sequence alignment