Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACHMXA_RS27695 Genome accession   NZ_CP171978
Coordinates   5886596..5887825 (+) Length   409 a.a.
NCBI ID   WP_395317133.1    Uniprot ID   -
Organism   Variovorax sp. UC74_104     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5881596..5892825
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXA_RS27665 (ACHMXA_27665) cgtA 5881673..5882749 (-) 1077 WP_176663132.1 Obg family GTPase CgtA -
  ACHMXA_RS27670 (ACHMXA_27670) rpmA 5882821..5883081 (-) 261 WP_093057448.1 50S ribosomal protein L27 -
  ACHMXA_RS27675 (ACHMXA_27675) rplU 5883103..5883414 (-) 312 WP_019656543.1 50S ribosomal protein L21 -
  ACHMXA_RS27680 (ACHMXA_27680) - 5883599..5884591 (+) 993 WP_395317131.1 polyprenyl synthetase family protein -
  ACHMXA_RS27690 (ACHMXA_27690) pilB 5884836..5886569 (+) 1734 WP_395317132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHMXA_RS27695 (ACHMXA_27695) pilC 5886596..5887825 (+) 1230 WP_395317133.1 type II secretion system F family protein Machinery gene
  ACHMXA_RS27700 (ACHMXA_27700) - 5887825..5888790 (+) 966 WP_395317134.1 prepilin peptidase -
  ACHMXA_RS27705 (ACHMXA_27705) coaE 5888798..5889409 (+) 612 WP_395317135.1 dephospho-CoA kinase -
  ACHMXA_RS27710 (ACHMXA_27710) - 5889479..5890972 (+) 1494 WP_395317136.1 MBOAT family O-acyltransferase -
  ACHMXA_RS27715 (ACHMXA_27715) - 5891134..5892201 (+) 1068 WP_395317137.1 hypothetical protein -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44804.47 Da        Isoelectric Point: 9.6798

>NTDB_id=1064719 ACHMXA_RS27695 WP_395317133.1 5886596..5887825(+) (pilC) [Variovorax sp. UC74_104]
MATVASPRTQPTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKAIKPKDIAIFTRQLA
TMMKAGVPLMQSFDIVGRGNANASVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEELLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMAMSEFFVSYWWLIFGGIGGGV
YFFLQAWKRNERFQRFMDRLLLRIPVFGVLIEKSCVARWTRTLATMFAAGVPLVEALDSVGGASGNTVYGDATAKIQQEV
STGTSLTSAMTNANLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGTIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1064719 ACHMXA_RS27695 WP_395317133.1 5886596..5887825(+) (pilC) [Variovorax sp. UC74_104]
ATGGCTACAGTGGCATCCCCCCGCACCCAGCCCACACACAAGGAATTTGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTACGCGGTGAACTTCGTGCCGCCGGCGAGAACCAGGTGCAGGCCGCACTGCGTCGCCAGGGCGTGCTCGCCT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGCAAGGCGATCAAGCCGAAGGACATCGCGATCTTCACGCGCCAGTTGGCG
ACCATGATGAAGGCCGGCGTGCCGCTGATGCAGTCCTTCGACATCGTGGGGCGCGGCAATGCCAACGCGAGCGTGGCCAA
GCTGCTCAACGACATCCGCAGCGATGTGGAAACCGGCACTTCGCTCTCGGCGGCCTTCCGCAAGTTTCCGAAGTACTTCG
ACAACCTCTACTGCAACCTGGTGGAAGCGGGCGAAGCAGCGGGTATTCTGGAAGAACTGCTGGACCGGCTGGCCACCTAC
ATGGAGAAGACCGAGGCGATCAAGTCGAAGATCAAGTCGGCGCTGATGTATCCCACTTCCGTGGTCGTCGTGGCGTTCGT
GGTGGTGGCGATCATCATGATCTTCGTGATCCCGGCGTTCAAGCAGGTGTTCACGTCGTTCGGAGCCGACCTGCCCGCTC
CGACGCTGTTCGTGATGGCGATGAGCGAGTTCTTCGTCTCCTACTGGTGGCTGATCTTCGGCGGCATCGGCGGCGGGGTC
TATTTCTTCCTGCAAGCCTGGAAGCGCAACGAACGCTTCCAGCGGTTCATGGACCGCCTCCTGCTGAGAATCCCGGTCTT
CGGCGTACTGATCGAAAAATCCTGCGTCGCCCGCTGGACCCGCACGCTGGCCACCATGTTCGCCGCCGGCGTCCCGCTGG
TCGAGGCCCTCGACTCCGTAGGCGGCGCTTCCGGCAACACCGTCTACGGCGACGCCACTGCGAAGATCCAGCAGGAGGTC
TCCACCGGCACCAGCCTCACGTCGGCCATGACAAATGCCAACCTGTTCCCGTCCATGGTGATCCAGATGACCGCCATCGG
GGAGGAATCCGGCTCCATCGACCACATGCTGGGCAAGGCCGCCGACTTCTACGAATCCGAAGTCGACGACATGGTCGCCG
GCCTCTCCAGCCTGATGGAGCCGATCATCATCGTGTTCCTCGGCACCATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.846

98.533

0.531
  pilG Neisseria gonorrhoeae MS11

52.645

97.066

0.511
  pilG Neisseria meningitidis 44/76-A

52.393

97.066

0.509
  pilC Acinetobacter baylyi ADP1

51.759

97.311

0.504
  pilC Legionella pneumophila strain ERS1305867

50.251

97.311

0.489
  pilC Acinetobacter baumannii D1279779

49.622

97.066

0.482
  pilC Vibrio cholerae strain A1552

41.709

97.311

0.406
  pilC Vibrio campbellii strain DS40M4

39.75

97.8

0.389
  pilC Thermus thermophilus HB27

37.947

100

0.389

Multiple sequence alignment