Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHMXA_RS27690 Genome accession   NZ_CP171978
Coordinates   5884836..5886569 (+) Length   577 a.a.
NCBI ID   WP_395317132.1    Uniprot ID   -
Organism   Variovorax sp. UC74_104     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5879836..5891569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHMXA_RS27660 (ACHMXA_27660) proB 5880441..5881583 (-) 1143 WP_395317130.1 glutamate 5-kinase -
  ACHMXA_RS27665 (ACHMXA_27665) cgtA 5881673..5882749 (-) 1077 WP_176663132.1 Obg family GTPase CgtA -
  ACHMXA_RS27670 (ACHMXA_27670) rpmA 5882821..5883081 (-) 261 WP_093057448.1 50S ribosomal protein L27 -
  ACHMXA_RS27675 (ACHMXA_27675) rplU 5883103..5883414 (-) 312 WP_019656543.1 50S ribosomal protein L21 -
  ACHMXA_RS27680 (ACHMXA_27680) - 5883599..5884591 (+) 993 WP_395317131.1 polyprenyl synthetase family protein -
  ACHMXA_RS27690 (ACHMXA_27690) pilB 5884836..5886569 (+) 1734 WP_395317132.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHMXA_RS27695 (ACHMXA_27695) pilC 5886596..5887825 (+) 1230 WP_395317133.1 type II secretion system F family protein Machinery gene
  ACHMXA_RS27700 (ACHMXA_27700) - 5887825..5888790 (+) 966 WP_395317134.1 prepilin peptidase -
  ACHMXA_RS27705 (ACHMXA_27705) coaE 5888798..5889409 (+) 612 WP_395317135.1 dephospho-CoA kinase -
  ACHMXA_RS27710 (ACHMXA_27710) - 5889479..5890972 (+) 1494 WP_395317136.1 MBOAT family O-acyltransferase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62516.67 Da        Isoelectric Point: 5.4455

>NTDB_id=1064718 ACHMXA_RS27690 WP_395317132.1 5884836..5886569(+) (pilB) [Variovorax sp. UC74_104]
MAAAELPVKEPSQIALPGLARALVSAGKLPAKAAEDIYQKSLSSRTSFIAELTGSGAVSAADLAHTLSTAFGAPLLDLDA
IDPQRLPKDLVDQKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
NSIVGSEFEFDDVSAETPSDSNDQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHNYRVRFRVDGELREVASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKAR
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNERAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCKLPADIPR
ETLLEAGFKEEELDGSWKPYRPVGCSACSSGYKGRVGIYQVMPISEAIQEIILRDGSALDIAAQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1064718 ACHMXA_RS27690 WP_395317132.1 5884836..5886569(+) (pilB) [Variovorax sp. UC74_104]
ATGGCCGCTGCCGAACTTCCTGTTAAAGAACCCTCGCAGATTGCCCTCCCAGGGCTTGCGCGCGCCCTGGTTTCGGCCGG
CAAGCTTCCTGCCAAGGCAGCCGAGGACATCTACCAGAAGTCCCTCAGTAGCCGTACCAGCTTCATCGCGGAGCTGACCG
GCTCCGGCGCCGTCTCGGCAGCCGACCTCGCCCACACCCTGTCCACGGCCTTCGGCGCCCCGCTGCTCGACCTGGACGCC
ATCGACCCTCAGCGCCTGCCGAAGGATCTGGTCGACCAGAAGCTGTGCCATGCGTACCGCATCGTCGTCCTCAGCAAGCG
CAACAACCGTCTGATCGTTGCAACAGCCGATCCGTCCGACCAGCAAGCGGCGGAAAAGATCAAGTTCGCCTCCCAGATGG
GCGTCGACTGGGTCATCGCCGAATACGACAAGCTGTCGCGAATGATCGAGGCCGCCGCCGTCAGCGCCGCGGAAACCATC
AACAGCATCGTCGGCAGCGAGTTCGAGTTCGACGATGTCTCGGCCGAAACCCCGAGCGACAGCAACGACCAGGCGATCGC
CGAAGTCGAGGACGCGCCGGTCGTTCGCTTCCTGCACAAGATGCTGCTGGACGCCGTCAGCATGCGGGCATCGGACATCC
ACTTCGAACCGTACGAACACAACTACCGCGTGCGCTTCCGCGTCGACGGCGAGTTGCGCGAGGTTGCGAGCCCGCCCACC
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATTTCCCGGCTCGACATCTCCGAGAAACGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGGCCCGACCGCGTGATCGACTTTCGCGTGAGCACCCTGCCCACGCTATTCGGCGAAAAGA
TCGTCGTCCGTATTCTCGATCCGAGCAGCGCGCGCCTGGGCATCGACGCCCTCGGCTACGACGCGGACGAGAAGGCACGG
CTGCTGCACGCCATCGGCCGTCCCTACGGCATGGTGCTGGTCACCGGTCCCACGGGCTCCGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACCAGCCGGGCGTCAACATCGCGACGGCGGAAGATCCGTCGGAAATCAACCTGCCCGGCG
TCAATCAGGTCAACGTCAACGAACGCGCGGGCCTGACCTTCGCGGCCGCGCTGCGCGCCTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAGACCGGCCACCTCGTGCT
CTCCACGCTGCACACCAACGACGCGCCGACCACGCTCACGCGGATGCGCAACATGGGCATCGCGCCGTTCAACATCGCCT
CGAGCGTGATCCTGATCACGGCGCAGCGCCTCGCGCGCCGCCTGTGCACCGTCTGCAAGCTCCCGGCCGACATTCCGCGC
GAAACGCTGCTCGAAGCAGGCTTCAAGGAAGAAGAACTGGACGGCTCCTGGAAGCCCTACCGCCCCGTCGGCTGCTCTGC
CTGCAGCAGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCGGAGGCCATCCAGGAAATCATCCTGC
GCGACGGCAGCGCACTCGACATCGCCGCGCAGTCGGAAGCCGAAGGCGTGCGCTCGCTGCGCCAGTCCGGCCTGCGCAAG
GTCATGCAGGGCCTCACTTCTCTGGAAGAAGTGGTGGCCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(54-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.516

97.4

0.541
  pilB Acinetobacter baylyi ADP1

54.982

97.4

0.536
  pilF Neisseria gonorrhoeae MS11

52.398

97.574

0.511
  pilB Legionella pneumophila strain ERS1305867

49.464

97.054

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.796

96.88

0.444
  pilB Vibrio campbellii strain DS40M4

45.455

97.227

0.442
  pilB Vibrio cholerae strain A1552

48.069

89.775

0.432
  pilF Thermus thermophilus HB27

38.07

98.787

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.338

92.374

0.373

Multiple sequence alignment