Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACHFJ9_RS20010 Genome accession   NZ_CP171887
Coordinates   2619176..2620861 (+) Length   561 a.a.
NCBI ID   WP_395356092.1    Uniprot ID   -
Organism   Vibrio sp. D3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2614176..2625861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACHFJ9_RS19990 (ACHFJ9_19990) pdhR 2615740..2616507 (-) 768 WP_005396680.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  ACHFJ9_RS19995 (ACHFJ9_19995) ampD 2616913..2617464 (-) 552 WP_256954321.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACHFJ9_RS20000 (ACHFJ9_20000) nadC 2617557..2618444 (+) 888 WP_395356088.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACHFJ9_RS20005 (ACHFJ9_20005) pilA 2618708..2619172 (+) 465 WP_395356090.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  ACHFJ9_RS20010 (ACHFJ9_20010) pilB 2619176..2620861 (+) 1686 WP_395356092.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACHFJ9_RS20015 (ACHFJ9_20015) pilC 2620885..2622108 (+) 1224 WP_017635918.1 type II secretion system F family protein Machinery gene
  ACHFJ9_RS20020 (ACHFJ9_20020) pilD 2622173..2623042 (+) 870 WP_395356094.1 prepilin peptidase Machinery gene
  ACHFJ9_RS20025 (ACHFJ9_20025) coaE 2623043..2623657 (+) 615 WP_047008979.1 dephospho-CoA kinase -
  ACHFJ9_RS20030 (ACHFJ9_20030) zapD 2623685..2624425 (+) 741 WP_005396691.1 cell division protein ZapD -
  ACHFJ9_RS20035 (ACHFJ9_20035) yacG 2624584..2624778 (+) 195 WP_005396692.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62277.09 Da        Isoelectric Point: 5.6670

>NTDB_id=1064652 ACHFJ9_RS20010 WP_395356092.1 2619176..2620861(+) (pilB) [Vibrio sp. D3]
MQTNLSTILRQKGFLTFSQEESLLEQVKASGISMPEALLSSGFFTSSELAEHLSSIFGLSQPELSQYEYASLCQQLGLRE
LITRHNALPLQRTPSTLLLAVADPTNQQAEDDFRFATGLQIELVLADFRQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNHDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1064652 ACHFJ9_RS20010 WP_395356092.1 2619176..2620861(+) (pilB) [Vibrio sp. D3]
ATGCAAACCAACCTCTCAACAATTCTTCGTCAAAAAGGATTTCTGACGTTTTCCCAAGAAGAATCGTTACTTGAGCAAGT
CAAAGCTTCTGGCATCTCGATGCCGGAAGCTTTACTCAGTTCTGGATTCTTCACGTCAAGCGAACTGGCCGAGCACTTAA
GTTCTATTTTTGGCTTAAGTCAGCCCGAGTTATCTCAGTATGAGTACGCTTCGCTTTGTCAACAACTCGGTCTACGTGAA
TTAATCACTCGACATAACGCACTCCCACTCCAGCGTACGCCTTCAACGTTGTTATTAGCCGTTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTCCGCTTTGCCACTGGGTTACAGATTGAACTGGTGCTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTATATGGTCGCTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATCAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGACGAATCTCCTGTTAGCCGTTACATAAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTTCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTAAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCAGAGCGACGTTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCACGACACCGCGAT
TGATATGCGTGTATCCACGCTACCGACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGCGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACTGGGAGCGGGAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTCTTACGTCAAGACCCCGATGTAGTAATGGTCGGTGAGATTCGTGATTTAGATACC
GCCGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTACATACCAATTCTGCAGCTGAAACGGT
GATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCCTCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGCACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGTGCCTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

96.078

100

0.961
  pilB Vibrio campbellii strain DS40M4

90.196

100

0.902
  pilB Vibrio cholerae strain A1552

74.377

100

0.745
  pilB Acinetobacter baumannii D1279779

51.036

94.652

0.483
  pilB Legionella pneumophila strain ERS1305867

48.566

99.465

0.483
  pilB Acinetobacter baylyi ADP1

47.576

99.287

0.472
  pilF Neisseria gonorrhoeae MS11

45.035

100

0.453
  pilB/pilB1 Synechocystis sp. PCC 6803

36.921

100

0.398
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.037

100

0.374
  pilF Thermus thermophilus HB27

36.299

100

0.364

Multiple sequence alignment