Detailed information NTG1064429

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PN837_RS13400
• Genome accession: NZ_CP171822
• Coordinates: 3193802..3194923 (-)
• Length: 373 a.a.
• NCBI ID: WP_395374069.1
• Uniprot ID: -
• Organism: Marinicella sp. W31
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3188802..3199923

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PN837_RS13385 (PN837_013385)	-	3189874..3191202 (+)	1329	WP_395374066.1	hypothetical protein	-
PN837_RS13390 (PN837_013390)	-	3191304..3193073 (+)	1770	WP_395374067.1	GspE/PulE family protein	-
PN837_RS13395 (PN837_013395)	-	3193063..3193797 (+)	735	WP_395374068.1	glutamine amidotransferase	-
PN837_RS13400 (PN837_013400)	pilU	3193802..3194923 (-)	1122	WP_395374069.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PN837_RS13405 (PN837_013405)	pilT	3194941..3195978 (-)	1038	WP_395374070.1	type IV pilus twitching motility protein PilT	Machinery gene
PN837_RS13410 (PN837_013410)	-	3196093..3196512 (+)	420	WP_395374071.1	DUF192 domain-containing protein	-
PN837_RS13415 (PN837_013415)	-	3196643..3198163 (+)	1521	WP_395374072.1	alanine/glycine:cation symporter family protein	-
PN837_RS13420 (PN837_013420)	gap	3198367..3199371 (+)	1005	WP_395374073.1	type I glyceraldehyde-3-phosphate dehydrogenase	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41596.73 Da
• Isoelectric Point: 6.5753

>NTDB_id=1064429 PN837_RS13400 WP_395374069.1 3193802..3194923(-) (pilU) [Marinicella sp. W31]
MDFNSYLKLMVHKKASDLFITANLPPSIKIHGRVVPITQNPLTKAQARDLVTGIMTPYQKEEFERTHECNFAIGVSSVGR
FRVSAFYQRNAVGMVIRRVESKIPTFESLHLPEVLKELSLTKRGIIIFVGGTGTGKSTSLAAMLGYRNVNTTGHIITIED
PIEYVHEHQGCVITQREVGIDTESFEVALRNTLRQAPDVIMIGEIRTKETMEQAITFAETGHLCLATLHANNANQALDRI
LHFFPKDSRDQLLMDLSLNLVSTVAQQLIPTPDGTGRRCAVEILINSPLASEYIRKGKIDKLKDLMKKSTNMGMRTFDQA
MFDLYQAGEITYEDALRHADSANEVRLAIKLSQGGDADSLAYGMDGVELEEER

Nucleotide

• Length: 1122 bp

>NTDB_id=1064429 PN837_RS13400 WP_395374069.1 3193802..3194923(-) (pilU) [Marinicella sp. W31]
ATGGACTTTAATTCATACCTCAAACTCATGGTTCACAAAAAGGCGTCGGATTTATTTATCACCGCCAACTTGCCGCCCAG
CATTAAGATTCACGGGCGCGTGGTACCCATTACTCAAAACCCGCTGACCAAGGCACAAGCCAGAGATTTGGTCACAGGCA
TTATGACGCCGTACCAAAAAGAAGAATTTGAAAGAACGCACGAATGTAACTTTGCCATTGGCGTATCCTCTGTGGGCCGC
TTCAGGGTCAGTGCCTTTTATCAGCGAAATGCTGTTGGTATGGTCATCCGGCGCGTAGAATCCAAAATTCCAACTTTTGA
ATCTCTACATTTGCCAGAAGTTCTGAAAGAATTATCACTAACCAAACGTGGCATTATCATCTTTGTAGGTGGTACAGGCA
CTGGTAAATCCACATCATTGGCAGCCATGCTCGGCTACCGAAATGTGAATACCACTGGACACATCATCACCATTGAAGAC
CCTATTGAATATGTGCATGAGCATCAAGGATGCGTTATTACACAGCGCGAAGTAGGTATTGATACCGAATCTTTTGAAGT
TGCCTTGAGAAACACGCTCCGGCAAGCCCCGGATGTGATCATGATCGGTGAGATCAGAACCAAGGAGACTATGGAGCAAG
CCATTACCTTTGCTGAAACAGGTCACCTGTGTCTGGCGACACTTCACGCCAACAATGCAAACCAGGCACTGGATCGCATT
TTGCACTTTTTCCCCAAAGACAGTCGTGATCAATTACTGATGGATTTGTCATTGAACTTGGTTTCAACAGTGGCACAACA
ATTGATACCAACACCGGATGGAACTGGCAGACGCTGCGCCGTGGAAATTCTCATTAATTCACCACTGGCTTCTGAATACA
TCCGTAAAGGTAAGATTGACAAGCTGAAAGATTTGATGAAAAAATCTACCAACATGGGCATGCGGACATTCGACCAGGCC
ATGTTTGATTTGTATCAGGCTGGCGAAATCACATACGAAGACGCTCTGCGACACGCAGATTCAGCCAACGAAGTTCGCTT
GGCCATTAAACTCAGTCAGGGCGGTGATGCCGACAGTCTTGCCTATGGTATGGACGGTGTAGAATTGGAAGAGGAACGCT
AA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	64.054	99.196	0.635
pilU	Acinetobacter baylyi ADP1	62.712	94.906	0.595
pilU	Vibrio cholerae strain A1552	54.875	96.247	0.528
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.563	95.174	0.386
pilT	Pseudomonas aeruginosa PAK	41.298	90.885	0.375
pilT	Acinetobacter nosocomialis M2	40.597	89.812	0.365
pilT	Acinetobacter baumannii D1279779	40.597	89.812	0.365
pilT	Acinetobacter baumannii strain A118	40.597	89.812	0.365