Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   ACE1MM_RS01865 Genome accession   NZ_CP171355
Coordinates   383544..384809 (-) Length   421 a.a.
NCBI ID   WP_062737996.1    Uniprot ID   A0AAJ4ZNM8
Organism   Ralstonia mannitolilytica strain SY001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 378544..389809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS01835 (ACE1MM_01835) - 379601..380485 (+) 885 WP_045785937.1 ATP-binding protein -
  ACE1MM_RS01840 (ACE1MM_01840) - 380475..380924 (+) 450 WP_045785938.1 NUDIX domain-containing protein -
  ACE1MM_RS01845 (ACE1MM_01845) yacG 380928..381134 (-) 207 WP_045785939.1 DNA gyrase inhibitor YacG -
  ACE1MM_RS01850 (ACE1MM_01850) zapD 381143..381901 (-) 759 WP_062737999.1 cell division protein ZapD -
  ACE1MM_RS01855 (ACE1MM_01855) coaE 382036..382650 (-) 615 WP_062737998.1 dephospho-CoA kinase -
  ACE1MM_RS01860 (ACE1MM_01860) pilD 382663..383544 (-) 882 WP_062737997.1 A24 family peptidase Machinery gene
  ACE1MM_RS01865 (ACE1MM_01865) pilG 383544..384809 (-) 1266 WP_062737996.1 type II secretion system F family protein Machinery gene
  ACE1MM_RS01870 (ACE1MM_01870) pilB 384854..386581 (-) 1728 WP_045785944.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACE1MM_RS01875 (ACE1MM_01875) - 386831..388105 (-) 1275 WP_045785945.1 HlyC/CorC family transporter -
  ACE1MM_RS01885 (ACE1MM_01885) ispB 388397..389326 (-) 930 WP_045787773.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46658.99 Da        Isoelectric Point: 10.3754

>NTDB_id=1060829 ACE1MM_RS01865 WP_062737996.1 383544..384809(-) (pilG) [Ralstonia mannitolilytica strain SY001]
MATRAPAANRAAAAPARKTAKTKAPTQYIFEWEGKDRKGKIFKGEMRAETITEVNAILRKQGLSITKSKRRRAARGKKIT
PKDIAYFTRQLSTMLKAGVPLLQSIDIIARGHANPNFTQLLTEIRMDIESGSSMAQAFRRHPRYFDTLYCNLIDAGEQGG
ILDALLERLSLYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKGVFSSFGATLPAPTLVVMSISDFFVQW
WYFIVFVPIIGIVAFIRAFKRSENVQRATHRWILKMPIFGDIIRKATIARWTRTLATMFAAGTPLVESMESVAGAAGNWV
YHDATIDIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDSMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=1060829 ACE1MM_RS01865 WP_062737996.1 383544..384809(-) (pilG) [Ralstonia mannitolilytica strain SY001]
ATGGCCACACGAGCACCAGCCGCAAACCGCGCGGCCGCCGCGCCGGCACGCAAGACCGCCAAGACGAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAAGACCGCAAAGGCAAGATCTTCAAGGGTGAAATGCGCGCCGAGACCATCACCGAGG
TCAACGCGATCCTGCGCAAGCAGGGGCTGTCGATCACCAAGTCCAAGCGCCGCCGTGCCGCGCGCGGCAAGAAGATCACG
CCGAAGGACATCGCGTACTTCACGCGCCAGCTCTCAACCATGCTCAAGGCCGGCGTGCCGCTGCTGCAGTCGATCGACAT
CATCGCGCGCGGCCACGCGAACCCGAACTTCACGCAGCTGCTGACCGAGATCCGCATGGACATCGAGTCGGGCAGCAGCA
TGGCGCAGGCGTTCCGACGCCACCCCCGGTACTTCGACACGCTCTATTGCAACCTCATCGACGCCGGTGAGCAGGGCGGT
ATCCTCGATGCGCTGCTCGAGCGGCTGTCGCTGTACATGGAAAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCCAT
GATCTACCCGATCTCGGTGCTGACGGTGGCGTTCCTGGTGACGGTGGTGCTGATGATCTTCGTGGTGCCGGCGTTCAAGG
GGGTGTTCTCGAGCTTCGGCGCCACGCTGCCCGCCCCGACGCTGGTGGTGATGAGCATCTCCGACTTCTTCGTGCAGTGG
TGGTACTTCATCGTCTTCGTGCCGATCATCGGCATCGTCGCATTTATCCGGGCATTCAAGCGATCGGAGAACGTGCAGCG
CGCCACGCACCGGTGGATCCTGAAAATGCCGATCTTCGGCGACATCATCCGCAAGGCGACCATCGCGCGCTGGACGCGCA
CGCTGGCGACGATGTTCGCGGCCGGCACGCCGCTGGTGGAATCGATGGAATCCGTGGCAGGTGCTGCCGGCAACTGGGTC
TATCACGACGCGACGATCGACATCCAGCAGGCGGTGCGCATCGGTACGAGCCTGACCAACGCCATGCAGGCCACCCACGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGTGAAGAGTCCGGTGCGCTGGACAGCATGCTGCTGAAGGTGGCCG
AGTTCTACGAGCGTGAAGTCGATGACGCCGTGGCGAATATCTCGGCGCTGATCGAGCCGATCATCATCGTGATCCTCGGC
GTGCTGATCGGCGGCATGGTGGTGGCGATGTATCTGCCGATCTTCAAGCTGGGTCAGGTGGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.128

94.774

0.485
  pilC Pseudomonas stutzeri DSM 10701

50.498

95.487

0.482
  pilG Neisseria meningitidis 44/76-A

50.877

94.774

0.482
  pilC Acinetobacter baumannii D1279779

47.174

96.675

0.456
  pilC Acinetobacter baylyi ADP1

46.929

96.675

0.454
  pilC Legionella pneumophila strain ERS1305867

47.03

95.962

0.451
  pilC Vibrio campbellii strain DS40M4

39.259

96.2

0.378
  pilC Vibrio cholerae strain A1552

39.348

94.774

0.373

Multiple sequence alignment