Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACE1MM_RS01870 Genome accession   NZ_CP171355
Coordinates   384854..386581 (-) Length   575 a.a.
NCBI ID   WP_045785944.1    Uniprot ID   A0AAJ4ZN22
Organism   Ralstonia mannitolilytica strain SY001     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 379854..391581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACE1MM_RS01840 (ACE1MM_01840) - 380475..380924 (+) 450 WP_045785938.1 NUDIX domain-containing protein -
  ACE1MM_RS01845 (ACE1MM_01845) yacG 380928..381134 (-) 207 WP_045785939.1 DNA gyrase inhibitor YacG -
  ACE1MM_RS01850 (ACE1MM_01850) zapD 381143..381901 (-) 759 WP_062737999.1 cell division protein ZapD -
  ACE1MM_RS01855 (ACE1MM_01855) coaE 382036..382650 (-) 615 WP_062737998.1 dephospho-CoA kinase -
  ACE1MM_RS01860 (ACE1MM_01860) pilD 382663..383544 (-) 882 WP_062737997.1 A24 family peptidase Machinery gene
  ACE1MM_RS01865 (ACE1MM_01865) pilG 383544..384809 (-) 1266 WP_062737996.1 type II secretion system F family protein Machinery gene
  ACE1MM_RS01870 (ACE1MM_01870) pilB 384854..386581 (-) 1728 WP_045785944.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACE1MM_RS01875 (ACE1MM_01875) - 386831..388105 (-) 1275 WP_045785945.1 HlyC/CorC family transporter -
  ACE1MM_RS01885 (ACE1MM_01885) ispB 388397..389326 (-) 930 WP_045787773.1 octaprenyl diphosphate synthase -
  ACE1MM_RS01890 (ACE1MM_01890) rplU 389645..389956 (+) 312 WP_004633023.1 50S ribosomal protein L21 -
  ACE1MM_RS01895 (ACE1MM_01895) rpmA 389994..390254 (+) 261 WP_009241587.1 50S ribosomal protein L27 -
  ACE1MM_RS01900 (ACE1MM_01900) obgE 390419..391519 (+) 1101 WP_045785947.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63728.66 Da        Isoelectric Point: 6.9137

>NTDB_id=1060830 ACE1MM_RS01870 WP_045785944.1 384854..386581(-) (pilB) [Ralstonia mannitolilytica strain SY001]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVASKYQMPLLDLAQYKLDTVPAVLTANKHF
AQLRLLPLARRENRLIVATSDPSDARPIEELRQKMNVTIETVIVEHDKLVRQLNLANEAPAEIKSLFPKQEAVQIEYDPG
AAAATRRTTADGIDDAPVVRFLQKLFTEALLRGASDLHFEPFETFYRVRFRIDGVLMQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDRKDKDNKETIDRAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNMLNTGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLARRLCKDCKKPGPLPKETL
LDAGFTEADLDGSWQPYHPVGCETCNGSGYKGRVGIYQVMPITEAIQEIILTHGTALQIAEQARKEGVLSLREAGLKKVK
LGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1060830 ACE1MM_RS01870 WP_045785944.1 384854..386581(-) (pilB) [Ralstonia mannitolilytica strain SY001]
ATGACTCTCGGACTCGCTCTAGCGCAGAGCCGGCGGATCGCGCCGACGTTACTCACCCAGCTCGAACAGAGCGCGCGTGA
AAAGCGCACGCAACTGATCGACGAACTCGTGAGCAGCGGGATCATGAGCGCTCACGACCTTGCCGTCTTCGTAGCCAGCA
AGTACCAGATGCCGCTGCTGGATCTGGCGCAGTACAAGCTGGATACGGTGCCGGCCGTGCTCACGGCCAACAAGCATTTC
GCGCAACTGCGCCTGCTGCCGCTGGCCCGGCGCGAAAACCGGCTGATCGTTGCCACGTCCGACCCCAGCGACGCGCGTCC
GATCGAAGAGTTGCGCCAGAAGATGAACGTGACGATCGAAACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAACTCA
ACCTGGCCAACGAAGCGCCGGCCGAGATCAAATCCCTCTTCCCCAAGCAGGAAGCCGTACAGATCGAATACGACCCGGGC
GCCGCGGCGGCCACGCGCCGCACCACGGCCGACGGCATCGACGATGCCCCCGTCGTGCGCTTTCTGCAGAAGCTCTTTAC
CGAAGCCCTGCTGCGCGGTGCTTCCGACCTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTTCGCATCGACG
GCGTGCTGATGCAGGTGGCCCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACGCGCATCAAGGTGCTCTCGCGCCTG
GACATTTCCGAGAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTGCTGATCACCGTCCCGAAGGACCGCAAGGACAAGGA
CAACAAGGAGACGATCGATCGCGCCATCGACTTCCGGGTCTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATCC
GGATTTTGGAGTCGTCCACCGAGCGGCTCGATATCGACCAGCTCGGCTACGAGCCCGAGCAGAAACAGCTGCTGCTCGAC
GTGATCAAGCGGCCGTACGGCATGGTGCTGGTGACGGGGCCGACAGGCTCGGGGAAGACGGTGTCGCTGTACACCTTCCT
CAACATGCTCAACACCGGCGACATCAACATTTCCACGGCAGAAGACCCGGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTGAACGACAAGGCGGGGCTGACGTTCGCGGCGGCACTCAAGTCGTTTTTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACGGCCGATATCTCGATCAAGGCCGCGCAGACGGGCCACCTGGTGTTCTCCACGCT
GCACACGAATGACGCTCCGACCACGCTCACGCGCCTGATGAACATGGGCGTGGCGCCGTTCAACATTGCGTCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGCGCGTCGGCTGTGCAAGGACTGCAAGAAACCGGGGCCGCTGCCGAAGGAAACGCTG
CTCGACGCCGGCTTTACCGAGGCCGACCTCGACGGCTCATGGCAACCGTATCACCCGGTCGGCTGCGAGACCTGCAACGG
CAGCGGCTACAAGGGGCGGGTCGGCATCTACCAGGTCATGCCGATTACGGAGGCGATCCAGGAGATCATCCTGACGCACG
GCACGGCGCTGCAGATTGCCGAGCAGGCGCGCAAGGAAGGCGTACTGTCGCTGCGTGAAGCGGGCCTGAAGAAAGTGAAG
CTGGGGCTGACTTCTCTCGAGGAAGTGCTGGCCACGACGAACCAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-134)

Type II/IV secretion system protein (T2SSE)

(184-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.482

98.087

0.515
  pilF Neisseria gonorrhoeae MS11

53.704

93.913

0.504
  pilB Acinetobacter baylyi ADP1

50.887

98.087

0.499
  pilB Legionella pneumophila strain ERS1305867

46.41

99.304

0.461
  pilB Vibrio cholerae strain A1552

44.737

99.13

0.443
  pilB Vibrio parahaemolyticus RIMD 2210633

45.155

95.13

0.43
  pilB Vibrio campbellii strain DS40M4

44.891

95.304

0.428
  pilB/pilB1 Synechocystis sp. PCC 6803

36.288

100

0.377
  pilF Thermus thermophilus HB27

38.612

97.739

0.377

Multiple sequence alignment