Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   ACF3NW_RS10215 Genome accession   NZ_CP171110
Coordinates   1993359..1994594 (+) Length   411 a.a.
NCBI ID   WP_314250914.1    Uniprot ID   -
Organism   Eikenella halliae strain WGS1533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1988359..1999594
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3NW_RS10205 (ACF3NW_10205) recJ 1989712..1991409 (+) 1698 WP_314250918.1 single-stranded-DNA-specific exonuclease RecJ -
  ACF3NW_RS10210 (ACF3NW_10210) pilF 1991652..1993328 (+) 1677 WP_314250916.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF3NW_RS10215 (ACF3NW_10215) pilG 1993359..1994594 (+) 1236 WP_314250914.1 type II secretion system F family protein Machinery gene
  ACF3NW_RS10220 (ACF3NW_10220) pilD 1994677..1995534 (+) 858 WP_314250912.1 A24 family peptidase Machinery gene
  ACF3NW_RS10225 (ACF3NW_10225) coaE 1995536..1996141 (+) 606 WP_314250910.1 dephospho-CoA kinase -
  ACF3NW_RS10230 (ACF3NW_10230) zapD 1996145..1996921 (+) 777 WP_314250908.1 cell division protein ZapD -
  ACF3NW_RS10235 (ACF3NW_10235) yacG 1996918..1997100 (+) 183 WP_003823528.1 DNA gyrase inhibitor YacG -
  ACF3NW_RS10240 (ACF3NW_10240) rlmD 1997167..1998459 (+) 1293 WP_392423317.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  ACF3NW_RS10245 (ACF3NW_10245) - 1998944..1999249 (-) 306 WP_314250901.1 hypothetical protein -

Sequence

Protein


Download         Length: 411 a.a.        Molecular weight: 45359.40 Da        Isoelectric Point: 9.4773

>NTDB_id=1060123 ACF3NW_RS10215 WP_314250914.1 1993359..1994594(+) (pilG) [Eikenella halliae strain WGS1533]
MATNKRNQSNKKVAGTSYEFEGRHMESEQIVRGEVVAKDEQDARAKLARRRIKVISLHKKKKVREKKITQGDITVFTRQL
ATMMKAGLPVMQAFDIVAKGHSNAAMTRLLMEVRNDIEQGTSMANAFRKHPKYFDDFYCNLVDAGEAGGVLESLLDKLAV
YKEKTLAIKKKIKSALTYPIAVVVVAIVLVMIMMMFVLPEFKKVYDGMNAPMPALTQFMMGISDFMVAWGWLVIIIMIAA
VAGFIQWHKLSPDLQKRVDGMLLKMPIFGNIVEKGTIARWARTTATLFTAGVPLVEALDSVGGASGNIIYEEATRSIRNQ
VNQGTSLTTAMSSTDLFPNMVLQMTSIGEESGALDDMLNKAAEFYEEEVDNAVATLSSLMEPIIMVVLGSIIGVILVAMY
LPLFNLGNVVG

Nucleotide


Download         Length: 1236 bp        

>NTDB_id=1060123 ACF3NW_RS10215 WP_314250914.1 1993359..1994594(+) (pilG) [Eikenella halliae strain WGS1533]
ATGGCTACAAACAAACGAAACCAATCCAATAAAAAAGTGGCGGGAACCAGCTACGAATTTGAAGGCCGGCACATGGAGTC
CGAGCAAATCGTCCGCGGCGAAGTAGTCGCCAAAGACGAACAGGATGCCCGTGCCAAACTCGCCCGCCGCCGCATCAAGG
TTATCAGCCTGCATAAAAAGAAAAAAGTTCGCGAAAAGAAAATCACCCAGGGCGACATTACCGTATTCACCCGCCAGCTG
GCCACCATGATGAAAGCTGGCCTACCAGTTATGCAGGCATTTGATATTGTTGCAAAAGGACACTCTAATGCCGCTATGAC
CAGGCTTTTAATGGAAGTGCGTAACGACATCGAGCAAGGTACCTCTATGGCCAATGCCTTCCGCAAACATCCCAAATATT
TTGATGATTTCTATTGCAACCTGGTGGATGCCGGTGAGGCAGGCGGTGTACTGGAAAGCTTGCTTGACAAACTGGCTGTA
TATAAAGAGAAAACCCTTGCCATCAAGAAAAAAATTAAAAGTGCATTAACCTATCCAATTGCCGTGGTAGTGGTAGCTAT
TGTCTTGGTCATGATCATGATGATGTTTGTATTGCCCGAGTTCAAAAAGGTATATGACGGGATGAACGCTCCCATGCCGG
CACTTACCCAATTCATGATGGGCATTTCTGACTTTATGGTAGCTTGGGGCTGGTTAGTTATTATCATCATGATTGCCGCT
GTTGCCGGATTTATTCAATGGCACAAACTCTCCCCTGACTTGCAAAAACGGGTCGATGGTATGCTGCTCAAAATGCCTAT
CTTCGGCAATATTGTCGAAAAAGGTACGATTGCTCGCTGGGCTCGTACCACCGCCACCTTGTTCACTGCCGGCGTTCCGT
TGGTAGAGGCGCTGGATTCTGTAGGTGGTGCTTCCGGCAACATCATTTACGAAGAAGCCACCAGAAGCATTAGAAATCAA
GTTAACCAGGGTACTTCGTTAACTACCGCCATGAGCTCAACCGACCTATTCCCCAATATGGTCTTGCAAATGACCTCAAT
TGGTGAAGAATCTGGTGCGCTGGACGATATGTTGAATAAAGCTGCTGAATTCTATGAAGAAGAGGTAGATAATGCCGTAG
CCACCCTATCTTCTCTGATGGAACCGATTATTATGGTGGTATTAGGTAGTATTATCGGTGTGATTCTGGTTGCCATGTAT
CTGCCCTTGTTCAACTTGGGTAACGTGGTTGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(280-402)

Type II secretion system (T2SS), protein F (T2SSF)

(76-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

65.217

95.134

0.62
  pilG Neisseria meningitidis 44/76-A

64.706

95.134

0.616
  pilC Legionella pneumophila strain ERS1305867

48.492

96.837

0.47
  pilC Pseudomonas stutzeri DSM 10701

45.185

98.54

0.445
  pilC Acinetobacter baumannii D1279779

45.113

97.08

0.438
  pilC Acinetobacter baylyi ADP1

45.455

93.674

0.426
  pilC Vibrio campbellii strain DS40M4

38.25

97.324

0.372
  pilC Vibrio cholerae strain A1552

38.095

97.08

0.37

Multiple sequence alignment