Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ACF3NW_RS10210 Genome accession   NZ_CP171110
Coordinates   1991652..1993328 (+) Length   558 a.a.
NCBI ID   WP_314250916.1    Uniprot ID   -
Organism   Eikenella halliae strain WGS1533     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1986652..1998328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACF3NW_RS10185 (ACF3NW_10185) - 1986773..1987327 (+) 555 WP_392423316.1 hypothetical protein -
  ACF3NW_RS10190 (ACF3NW_10190) fumC 1987680..1989074 (-) 1395 WP_392423872.1 class II fumarate hydratase -
  ACF3NW_RS10205 (ACF3NW_10205) recJ 1989712..1991409 (+) 1698 WP_314250918.1 single-stranded-DNA-specific exonuclease RecJ -
  ACF3NW_RS10210 (ACF3NW_10210) pilF 1991652..1993328 (+) 1677 WP_314250916.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACF3NW_RS10215 (ACF3NW_10215) pilG 1993359..1994594 (+) 1236 WP_314250914.1 type II secretion system F family protein Machinery gene
  ACF3NW_RS10220 (ACF3NW_10220) pilD 1994677..1995534 (+) 858 WP_314250912.1 A24 family peptidase Machinery gene
  ACF3NW_RS10225 (ACF3NW_10225) coaE 1995536..1996141 (+) 606 WP_314250910.1 dephospho-CoA kinase -
  ACF3NW_RS10230 (ACF3NW_10230) zapD 1996145..1996921 (+) 777 WP_314250908.1 cell division protein ZapD -
  ACF3NW_RS10235 (ACF3NW_10235) yacG 1996918..1997100 (+) 183 WP_003823528.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62122.84 Da        Isoelectric Point: 5.1922

>NTDB_id=1060122 ACF3NW_RS10210 WP_314250916.1 1991652..1993328(+) (pilF) [Eikenella halliae strain WGS1533]
MSVGLLRILFQNKLIEQDQVEKYREKVNQNKNIIPMLAEAGIIKQADLALLLAKLFHYPILDLSLYSRSMMVPDVINDSQ
MTEHRCIPLFKRGQKLFLGVSDPTLLQTYQKLVFPSGLIVDLILVNDEQLDRLLEFASQTSTAILDEISADTTGLAAPAS
LKVDDEEEDGPIAKFIYKVLSDALNTGASDIHFEFYEEMARIRFRTDGQLREVVQPPLNIRGQIASRIKVMSKMDISEKR
VPQDGRIQLQFNKNQKPIDFRVNTLPTLFGEKIVMRILNSDAATLNINQLGFEPFQKELLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTDSVNISTAEDPAEINLPGINQVNVNDKQGMTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLVRRLCTCKQPIERPPAEALLKVGFTEEDLAKDDWQLY
RQVGCDRCKGKGFKGRAGVYEIMPVTDAMQKLIINNGTEVDIAEQAYADGLVDLRRAGILKAMQGITTLEEILATTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1060122 ACF3NW_RS10210 WP_314250916.1 1991652..1993328(+) (pilF) [Eikenella halliae strain WGS1533]
ATGAGCGTCGGACTATTGCGCATCCTGTTTCAAAACAAACTGATTGAGCAGGATCAGGTTGAAAAATACCGAGAAAAAGT
TAACCAAAACAAAAACATTATCCCCATGCTGGCTGAGGCCGGCATCATCAAGCAGGCAGATTTGGCTCTGCTTTTGGCCA
AACTATTCCACTATCCGATTTTGGATTTGTCCCTCTACTCCCGCAGCATGATGGTGCCGGACGTGATCAACGACAGCCAG
ATGACAGAGCACCGCTGCATCCCCTTATTCAAACGCGGGCAAAAACTGTTTTTGGGTGTCTCCGACCCTACTTTGTTGCA
AACATACCAAAAACTTGTTTTCCCCAGCGGCCTGATTGTCGACCTGATTTTGGTAAACGACGAACAACTAGACCGCCTGC
TGGAATTCGCCAGCCAAACATCCACTGCTATTTTGGATGAAATCTCTGCCGACACAACAGGCCTAGCCGCACCGGCATCA
CTAAAAGTGGATGACGAAGAAGAAGACGGCCCAATTGCCAAATTCATCTACAAAGTGCTGTCCGATGCCCTCAATACCGG
CGCATCCGATATTCACTTCGAGTTCTATGAAGAAATGGCACGTATCCGCTTCCGTACCGATGGCCAGTTACGCGAAGTGG
TGCAGCCGCCTCTGAATATCCGCGGACAGATTGCCTCACGTATCAAGGTGATGTCCAAAATGGATATTTCCGAAAAGCGC
GTTCCGCAAGACGGCCGTATCCAGCTTCAGTTCAATAAAAACCAAAAACCCATCGACTTCCGTGTGAATACGCTGCCCAC
GCTGTTCGGCGAAAAAATCGTAATGCGTATTTTGAATTCAGATGCCGCCACGTTGAATATCAACCAACTGGGTTTCGAAC
CTTTCCAAAAAGAATTGCTGCTGGAAGCCATCCACCGCCCGTACGGCATGGTGCTGGTTACCGGCCCGACCGGTTCAGGT
AAAACCGTGTCGCTGTACACCTGTCTAAACATCTTGAATACCGACAGCGTAAACATCTCCACCGCCGAAGACCCGGCTGA
GATTAACTTGCCCGGTATCAATCAGGTAAATGTAAACGACAAACAAGGCATGACATTTGCTGCGGCACTGAAAGCCTTCC
TGCGTCAAGACCCGGACATCATCATGGTGGGTGAGATTCGCGATTTGGAAACTGCCGATATCGCCATTAAAGCAGCGCAA
ACCGGCCACATGGTATTCTCCACCCTACACACCAATAATGCTCCGGCCACGCTATCCCGTATGCTGAACATGGGGGTAGC
ACCGTTCAATATCGCCAGCTCGGTCAACCTGATCATGGCACAGCGCTTGGTGCGTCGGCTGTGTACCTGCAAACAACCGA
TCGAACGTCCGCCAGCCGAAGCTTTGCTAAAAGTCGGCTTCACCGAAGAAGACTTGGCCAAGGACGACTGGCAGCTATAC
CGCCAGGTGGGTTGCGACCGTTGCAAAGGCAAAGGCTTTAAGGGCCGTGCCGGCGTATACGAAATTATGCCGGTTACCGA
TGCCATGCAAAAACTTATTATCAATAACGGCACCGAGGTGGATATTGCCGAGCAAGCCTATGCCGACGGGCTGGTGGACT
TACGCCGTGCCGGCATTTTGAAAGCAATGCAAGGCATTACCACCTTAGAAGAAATTCTTGCCACCACTAACGACTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-135)

Type II/IV secretion system protein (T2SSE)

(178-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.821

100

0.701
  pilB Acinetobacter baylyi ADP1

50.883

100

0.516
  pilB Acinetobacter baumannii D1279779

50.887

100

0.514
  pilB Legionella pneumophila strain ERS1305867

46.41

100

0.475
  pilB Vibrio cholerae strain A1552

46.303

100

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

44.523

100

0.452
  pilB Vibrio campbellii strain DS40M4

43.717

100

0.443
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.19

94.086

0.378

Multiple sequence alignment