Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACFE3T_RS03165 Genome accession   NZ_CP170742
Coordinates   645027..646136 (-) Length   369 a.a.
NCBI ID   WP_392400381.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain XN-7     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 640027..651136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFE3T_RS03140 (ACFE3T_03140) - 640168..640398 (+) 231 WP_021140462.1 hypothetical protein -
  ACFE3T_RS03145 (ACFE3T_03145) brnQ 640474..641787 (-) 1314 WP_257978485.1 branched-chain amino acid transport system II carrier protein -
  ACFE3T_RS03150 (ACFE3T_03150) - 641973..642683 (-) 711 WP_219593025.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  ACFE3T_RS03155 (ACFE3T_03155) srmB 642811..644034 (+) 1224 WP_005320462.1 ATP-dependent RNA helicase SrmB -
  ACFE3T_RS03160 (ACFE3T_03160) yaaA 644222..644995 (-) 774 WP_125608785.1 peroxide stress protein YaaA -
  ACFE3T_RS03165 (ACFE3T_03165) pilU 645027..646136 (-) 1110 WP_392400381.1 type IVa pilus ATPase TapU Machinery gene
  ACFE3T_RS03170 (ACFE3T_03170) pilT 646172..647206 (-) 1035 WP_005320466.1 type IVa pilus ATPase TapT Machinery gene
  ACFE3T_RS03175 (ACFE3T_03175) - 647246..647947 (+) 702 WP_058395315.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACFE3T_RS03180 (ACFE3T_03180) proC 648038..648862 (+) 825 WP_113720586.1 pyrroline-5-carboxylate reductase -
  ACFE3T_RS03185 (ACFE3T_03185) - 648891..649442 (+) 552 WP_219593022.1 YggT family protein -
  ACFE3T_RS03190 (ACFE3T_03190) yggU 649442..649741 (+) 300 WP_005320479.1 DUF167 family protein YggU -
  ACFE3T_RS03195 (ACFE3T_03195) - 649760..650179 (+) 420 WP_005320482.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41073.08 Da        Isoelectric Point: 6.3715

>NTDB_id=1059078 ACFE3T_RS03165 WP_392400381.1 645027..646136(-) (pilU) [Aeromonas salmonicida strain XN-7]
MNLDALLAELVARKGSDLFITVGTPPTLKINGHLFSLGSEALDKQSALTLVRDTLSPEHFERYIRTREGNYAIHREALGR
FRVSAFWQQELPGMVVRRIETRIPTFDELTLPPILQEVAMAKRGLVLFVGATGTGKSTTQAAMIGYRNQNADGHILTVED
PVEFVHQHGRSLITQREVGIDTESFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQLLFDLSFNLRAIVAQQLVPSLDGRRRFAAFEVLLNTPLITDIIRKGEVHRLKEVMTKSTELGMQTFDQA
LFTLFCAGQIGYSEALAHADSANDLRLLIKLSGREQMGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1059078 ACFE3T_RS03165 WP_392400381.1 645027..646136(-) (pilU) [Aeromonas salmonicida strain XN-7]
ATGAATCTGGATGCCCTGTTGGCCGAACTGGTCGCACGCAAAGGCTCGGATCTGTTCATCACGGTTGGGACGCCCCCCAC
CCTCAAGATCAACGGGCATCTGTTCTCCCTCGGCAGCGAAGCGCTCGACAAGCAGAGTGCGCTGACCCTGGTCAGGGACA
CGCTCAGCCCTGAGCATTTCGAGCGCTACATCCGCACCAGGGAGGGCAACTACGCCATCCACCGCGAGGCACTGGGACGC
TTTCGGGTGAGTGCCTTCTGGCAGCAGGAGCTGCCGGGCATGGTGGTGCGCCGTATCGAGACCCGCATTCCAACCTTTGA
CGAACTGACCCTGCCGCCCATCTTGCAGGAGGTGGCCATGGCCAAACGCGGCCTGGTGCTGTTCGTCGGTGCCACCGGAA
CGGGCAAATCCACCACTCAGGCAGCCATGATCGGCTATCGAAACCAGAATGCGGATGGCCATATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGGGAAGTGGGTATAGATACGGAATCATTCGATGT
GGCGCTGAAGAGCTCCCTGCGCCAGGCACCTGACGTTATCCTCATCGGCGAGATCCGAAGCCAGGAAACCATGGAGTTCG
CACTGCAATTTGCCGAGACCGGCCACCTTTGCCTCGCGACCCTGCACGCCAACAATGCCAACCAGGCGCTGGATCGCATC
CTCCATCTGGTGCCGCAGGAGAAACACCGTCAGTTACTGTTCGATCTCTCCTTCAACCTGCGCGCCATCGTCGCCCAGCA
ACTGGTGCCAAGCCTGGATGGTCGCCGCCGCTTCGCGGCCTTTGAAGTACTGCTCAACACGCCGCTCATCACGGATATCA
TCCGCAAAGGCGAGGTTCATCGTCTCAAGGAGGTGATGACCAAATCCACCGAGCTTGGCATGCAGACCTTCGATCAGGCG
CTGTTTACCCTGTTTTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCCGGCCGTGAGCAGATGGGTGCAGGCACACTCGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

59.511

99.729

0.593
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.79

95.664

0.553
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.233

92.954

0.374
  pilT Legionella pneumophila strain ERS1305867

40.233

92.954

0.374
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363

Multiple sequence alignment