Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACFJIS_RS02395 Genome accession   NZ_CP170558
Coordinates   488591..489820 (+) Length   409 a.a.
NCBI ID   WP_390346169.1    Uniprot ID   -
Organism   Variovorax boronicumulans strain UC19_11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483591..494820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIS_RS02365 (ACFJIS_02365) cgtA 483680..484756 (-) 1077 WP_062474512.1 Obg family GTPase CgtA -
  ACFJIS_RS02370 (ACFJIS_02370) rpmA 484829..485089 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  ACFJIS_RS02375 (ACFJIS_02375) rplU 485102..485413 (-) 312 WP_062474515.1 50S ribosomal protein L21 -
  ACFJIS_RS02380 (ACFJIS_02380) - 485596..486588 (+) 993 WP_390350054.1 polyprenyl synthetase family protein -
  ACFJIS_RS02390 (ACFJIS_02390) pilB 486833..488566 (+) 1734 WP_062474518.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFJIS_RS02395 (ACFJIS_02395) pilC 488591..489820 (+) 1230 WP_390346169.1 type II secretion system F family protein Machinery gene
  ACFJIS_RS02400 (ACFJIS_02400) - 489820..490785 (+) 966 WP_390346171.1 prepilin peptidase -
  ACFJIS_RS02405 (ACFJIS_02405) coaE 490799..491407 (+) 609 WP_390346173.1 dephospho-CoA kinase -
  ACFJIS_RS02410 (ACFJIS_02410) - 491484..492611 (+) 1128 WP_390346174.1 MBOAT family O-acyltransferase -
  ACFJIS_RS02415 (ACFJIS_02415) - 492593..492976 (+) 384 WP_390346176.1 hypothetical protein -
  ACFJIS_RS02420 (ACFJIS_02420) - 492983..494014 (+) 1032 WP_390346177.1 hypothetical protein -
  ACFJIS_RS02425 (ACFJIS_02425) zapD 494051..494807 (+) 757 Protein_480 cell division protein ZapD -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 44787.53 Da        Isoelectric Point: 9.6628

>NTDB_id=1057998 ACFJIS_RS02395 WP_390346169.1 488591..489820(+) (pilC) [Variovorax boronicumulans strain UC19_11]
MATVASSRSQVTHKEFVFEWEGKDRNGKLVRGELRAAGENQVQAALRRQGVLASKIKKRRMRSGKKIKPKDIAIFTRQLA
TMMKAGVPLLQSFDIVGRGNANPNVAKLLNDVRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEELLDRLATY
MEKTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKEVFTSFGADLPAPTLFVMAISEFFVSYWWLIFGVLGGGI
YFFLQAWKRNERVQKVMDRLLLRLPVFGTLIEKSCIARWTRTLATMFAAGVPLVEALDSVGGASGNSVYGDATAKIQQEV
STGTSLTAAMTNVNLFPSMVIQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYL
PIFKLGQVV

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1057998 ACFJIS_RS02395 WP_390346169.1 488591..489820(+) (pilC) [Variovorax boronicumulans strain UC19_11]
ATGGCAACAGTGGCATCCTCCCGCTCCCAGGTCACGCACAAGGAATTCGTCTTCGAATGGGAAGGCAAGGACCGCAACGG
CAAGCTGGTACGCGGCGAACTTCGCGCCGCCGGGGAGAACCAGGTGCAGGCGGCCTTGCGTCGCCAGGGCGTGCTGGCCT
CCAAGATCAAGAAGCGCCGCATGCGCTCGGGTAAGAAAATCAAGCCCAAGGACATCGCGATCTTCACGCGCCAGCTCGCG
ACCATGATGAAGGCCGGCGTGCCGCTGCTGCAGTCCTTCGACATCGTCGGCCGCGGCAACGCGAACCCGAACGTCGCCAA
ATTGCTGAACGATGTCCGCAGCGATGTCGAGACCGGCACCTCGCTGTCGGCCGCTTTTCGCAAGTTTCCGAAGTACTTCG
ACAACCTATACTGCAACCTTGTGGAGGCCGGTGAGGCTGCCGGTATCCTGGAAGAGTTGCTGGACCGCCTCGCGACCTAC
ATGGAGAAGACCGAGGCAATCAAGTCGAAGATCAAGTCGGCACTGATGTACCCGACTTCGGTGGTCGTCGTGGCCTTCGT
GGTGGTGGCAATCATCATGATCTTCGTGATTCCCGCGTTCAAGGAGGTGTTCACCTCATTCGGCGCCGATTTGCCTGCGC
CGACGTTGTTCGTCATGGCGATCAGCGAGTTTTTTGTTTCCTATTGGTGGCTGATCTTCGGCGTGCTCGGCGGGGGCATC
TACTTCTTCCTCCAAGCTTGGAAACGCAACGAACGCGTCCAGAAGGTCATGGATCGCCTGCTGCTGCGCCTCCCGGTTTT
CGGCACTCTGATTGAGAAGTCCTGCATCGCCCGCTGGACCCGCACGCTCGCCACCATGTTCGCAGCCGGCGTGCCGCTGG
TCGAGGCCCTCGATTCCGTGGGCGGCGCATCGGGCAACTCGGTCTACGGCGACGCCACCGCCAAGATCCAGCAGGAGGTT
TCGACCGGCACCAGCCTCACGGCCGCCATGACCAACGTCAACCTGTTCCCTTCGATGGTGATCCAGATGACGGCCATCGG
CGAGGAGTCGGGCTCGATCGATCACATGCTCGGCAAGGCCGCCGACTTCTACGAATCCGAAGTCGACGACATGGTCGCTG
GCCTCTCCAGCCTGATGGAGCCCATCATCATCGTGTTCCTGGGCGTCATCATCGGCGGCATCGTGGTGTCGATGTACCTG
CCCATCTTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(279-401)

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.208

98.778

0.535
  pilC Acinetobacter baylyi ADP1

51.338

100

0.516
  pilG Neisseria gonorrhoeae MS11

52.645

97.066

0.511
  pilG Neisseria meningitidis 44/76-A

52.393

97.066

0.509
  pilC Legionella pneumophila strain ERS1305867

50.251

97.311

0.489
  pilC Acinetobacter baumannii D1279779

50.126

97.066

0.487
  pilC Vibrio cholerae strain A1552

40.955

97.311

0.399
  pilC Thermus thermophilus HB27

38.663

100

0.396
  pilC Vibrio campbellii strain DS40M4

39.303

98.289

0.386

Multiple sequence alignment