Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACFJIS_RS02390 Genome accession   NZ_CP170558
Coordinates   486833..488566 (+) Length   577 a.a.
NCBI ID   WP_062474518.1    Uniprot ID   A0A554S5W8
Organism   Variovorax boronicumulans strain UC19_11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481833..493566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJIS_RS02360 (ACFJIS_02360) proB 482460..483599 (-) 1140 WP_062474510.1 glutamate 5-kinase -
  ACFJIS_RS02365 (ACFJIS_02365) cgtA 483680..484756 (-) 1077 WP_062474512.1 Obg family GTPase CgtA -
  ACFJIS_RS02370 (ACFJIS_02370) rpmA 484829..485089 (-) 261 WP_013543198.1 50S ribosomal protein L27 -
  ACFJIS_RS02375 (ACFJIS_02375) rplU 485102..485413 (-) 312 WP_062474515.1 50S ribosomal protein L21 -
  ACFJIS_RS02380 (ACFJIS_02380) - 485596..486588 (+) 993 WP_390350054.1 polyprenyl synthetase family protein -
  ACFJIS_RS02390 (ACFJIS_02390) pilB 486833..488566 (+) 1734 WP_062474518.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACFJIS_RS02395 (ACFJIS_02395) pilC 488591..489820 (+) 1230 WP_390346169.1 type II secretion system F family protein Machinery gene
  ACFJIS_RS02400 (ACFJIS_02400) - 489820..490785 (+) 966 WP_390346171.1 prepilin peptidase -
  ACFJIS_RS02405 (ACFJIS_02405) coaE 490799..491407 (+) 609 WP_390346173.1 dephospho-CoA kinase -
  ACFJIS_RS02410 (ACFJIS_02410) - 491484..492611 (+) 1128 WP_390346174.1 MBOAT family O-acyltransferase -
  ACFJIS_RS02415 (ACFJIS_02415) - 492593..492976 (+) 384 WP_390346176.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62568.87 Da        Isoelectric Point: 5.8808

>NTDB_id=1057997 ACFJIS_RS02390 WP_062474518.1 486833..488566(+) (pilB) [Variovorax boronicumulans strain UC19_11]
MAAAEIPVKETTQIALPGLARALVSAGKLAAKTAEDIYQKSLSGRTSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDHQRLPKDLLDPKLCHAYRIVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAASAAETI
NSIVGTEFEFDDVTADTSGDANEQAIAEVEDAPVVRFLHKMLLDAVSMRASDIHFEPYEHHYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARMGIDALGYDADEKER
LLHAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCMLCRAPIDIPR
EALLDAGFKEEDINGTWKPYRPVGCAACSGGYKGRVGIYQVMPISEAIQEIILRDGSALDIANQSEAEGVRSLRQSGLRK
VMQGLTSLEEVVACTNL

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1057997 ACFJIS_RS02390 WP_062474518.1 486833..488566(+) (pilB) [Variovorax boronicumulans strain UC19_11]
ATGGCCGCTGCCGAAATTCCCGTTAAAGAAACTACGCAGATCGCCCTTCCCGGCTTGGCGCGCGCCCTCGTGTCGGCCGG
CAAGCTCGCGGCGAAGACAGCCGAAGACATCTATCAGAAGTCGCTGAGCGGCCGCACCAGCTTCATCGCCGAGCTGACCG
GCACCGGCGCCGTCTCGGCGGCGGACCTCGCCCACACTCTCTCAAGCGCCTTCGGCGCCCCGCTGCTCGACCTCGATGCC
ATCGATCACCAGCGCCTGCCCAAGGACCTGCTCGATCCGAAGCTCTGCCATGCGTACCGCATCGTCGTTCTCAGCAAGCG
CAACAACCGCCTGATCGTCGCGACGGCCGACCCGTCGGACCAGCAAGCCGCGGAGAAAATCAAGTTCGCGTCGCAGATGG
GCGTGGACTGGGTCATCGCGGAGTACGACAAGCTCTCGCGCATGATCGAAGCCGCTGCAGCGAGCGCGGCTGAAACCATC
AACAGCATCGTCGGCACCGAGTTCGAATTCGACGATGTCACGGCCGACACCTCGGGCGATGCCAACGAGCAAGCCATCGC
CGAAGTCGAGGACGCACCCGTGGTGCGCTTCCTGCACAAAATGCTGCTCGACGCCGTCAGCATGCGGGCCTCGGACATCC
ACTTCGAGCCCTACGAACACCACTACCGGGTGCGCTTCCGGGTCGATGGCGAGCTGCGCGAAATCGCCAGTCCGCCCACG
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTCATTTCGCGGCTCGACATCTCGGAAAAACGCGTGCCGCAAGACGG
CCGCATGAAGCTCAAGATCGGCCCCGACCGCGTGATCGACTTCCGCGTGAGCACGCTGCCCACGCTGTTCGGCGAGAAGA
TCGTCGTGCGTATCCTGGACCCGAGCAGCGCGCGCATGGGCATCGACGCCCTAGGCTACGACGCCGACGAAAAAGAACGC
CTCCTTCACGCCATCGGCCGCCCCTACGGCATGGTGCTGGTGACCGGCCCCACAGGTTCGGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAACCTGCTCAACCAGCCGGGCGTCAACATCGCGACGGCGGAAGACCCGTCGGAAATCAACCTGCCCGGCG
TCAACCAGGTCAACGTCAACGAGAAGGCCGGCCTCACTTTCGCGGCCGCGCTGCGTGCCTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGACATCTCGATCAAGGCCGCGCAAACCGGTCACCTGGTGCT
GTCGACGCTGCACACCAACGACGCCCCGACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACATCGCGT
CGAGCGTGATCCTGATCACTGCGCAACGCCTCGCGCGCCGGCTGTGCATGCTGTGCCGCGCGCCGATCGACATCCCGCGC
GAAGCCCTGCTCGATGCCGGCTTCAAAGAGGAAGACATCAACGGCACCTGGAAGCCCTACCGCCCGGTCGGCTGCGCCGC
CTGCAGCGGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCGATTTCTGAAGCCATCCAGGAAATCATCCTGC
GCGACGGCAGCGCGCTGGACATCGCGAACCAATCCGAAGCCGAAGGCGTGCGCTCGCTGCGCCAGTCTGGCCTTCGCAAA
GTCATGCAGGGCCTCACTTCGCTCGAAGAAGTCGTCGCCTGCACCAACCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-157)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A554S5W8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.31

99.48

0.53
  pilB Acinetobacter baumannii D1279779

54.107

97.054

0.525
  pilF Neisseria gonorrhoeae MS11

52.669

97.4

0.513
  pilB Legionella pneumophila strain ERS1305867

48.496

97.92

0.475
  pilB Vibrio cholerae strain A1552

46.607

97.054

0.452
  pilB Vibrio campbellii strain DS40M4

45.826

97.574

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

45.276

97.227

0.44
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.254

97.574

0.383
  pilF Thermus thermophilus HB27

37.786

98.614

0.373

Multiple sequence alignment