Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB5G97_RS14150 Genome accession   NZ_CP170371
Coordinates   3020424..3021563 (+) Length   379 a.a.
NCBI ID   WP_005353124.1    Uniprot ID   A0A0T6QZZ8
Organism   Aeromonas veronii strain Aer12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3015424..3026563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB5G97_RS14120 (AB5G97_14120) - 3015494..3015874 (-) 381 WP_005337641.1 diacylglycerol kinase -
  AB5G97_RS14125 (AB5G97_14125) - 3016019..3017080 (+) 1062 WP_158109342.1 3-deoxy-7-phosphoheptulonate synthase -
  AB5G97_RS14130 (AB5G97_14130) - 3017197..3017994 (-) 798 WP_376870054.1 ATP-binding cassette domain-containing protein -
  AB5G97_RS14135 (AB5G97_14135) btuC 3018060..3019067 (-) 1008 WP_158109343.1 vitamin B12 ABC transporter permease BtuC -
  AB5G97_RS14140 (AB5G97_14140) - 3019165..3019896 (-) 732 WP_043825634.1 ABC transporter substrate-binding protein -
  AB5G97_RS14145 (AB5G97_14145) - 3020009..3020365 (+) 357 WP_005353120.1 DMT family protein -
  AB5G97_RS14150 (AB5G97_14150) pilU 3020424..3021563 (+) 1140 WP_005353124.1 type IVa pilus ATPase TapW Machinery gene
  AB5G97_RS14155 (AB5G97_14155) - 3021659..3022108 (-) 450 WP_236380883.1 isoprenylcysteine carboxylmethyltransferase family protein -
  AB5G97_RS14160 (AB5G97_14160) alr 3022282..3023508 (+) 1227 WP_274160279.1 alanine racemase -
  AB5G97_RS14165 (AB5G97_14165) - 3023574..3024518 (-) 945 WP_005353134.1 TerC family protein -
  AB5G97_RS14170 (AB5G97_14170) oppF 3024821..3025816 (-) 996 WP_005337666.1 murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42253.34 Da        Isoelectric Point: 6.6011

>NTDB_id=1056367 AB5G97_RS14150 WP_005353124.1 3020424..3021563(+) (pilU) [Aeromonas veronii strain Aer12]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLNETPLEPGEVARIAAEIMDGEQKQQFEKDLEMNLAISLPQIGR
FRVNIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGGLKEVMEKSKALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIASSSDATGWSLEPTKDEKGDFF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1056367 AB5G97_RS14150 WP_005353124.1 3020424..3021563(+) (pilU) [Aeromonas veronii strain Aer12]
ATGGAATTGCGAGACATGTTACAGATCCTGGCCAAGCAGGATGGCTCAGACCTCTATCTGTCGACCGGCGCGCCCCCCTG
CGCCAAATTTAACGGCGGCCTGCGCCCCCTCAACGAAACGCCGCTGGAACCGGGTGAAGTGGCGCGGATCGCCGCCGAGA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGAAGGATCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATCGGCCGC
TTTCGGGTCAACATCTTCAAGCAGCGCAACGAAGTCTCTCTGGTGGCCCGCAACATCAAGACCGAAATCCCCCGCTTTGA
AGATCTCAAACTCCCGCCTGTGCTGCTCGACACCATCATGGAGAAACGAGGCCTGGTGCTGTTTGTCGGCGGTACCGGCT
CGGGCAAATCCACTTCGCTGGCGGCGCTTATAGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACAATCGAAGAT
CCGGTAGAGTTCGTTCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTTGGCGTGGATACCCGCAGCTTCCACGC
AGCCCTGAAAAACACCCTGCGTCAGGCGCCGGATGTCATTCTTATCGGCGAGATCCGCGATCGCGAGACCATGGAACATG
CGCTGGCCTTCTCCGAAACCGGCCACCTTGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGCCCCCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCTTTTGTCTCCCAACG
GCTGGTGAAAACCACCGATGGCGGCCGCCGGGCCGCAGTGGAGATCATGCTCGGCACCCACACCATCCGCGACATGATCA
AGCGCGGCGAATTTGGCGGCCTCAAAGAGGTGATGGAAAAGTCCAAAGCCCTCGGCATGGTCACCTTCGACAGTGCCCTG
TTTGATCTGGTGGTGGAAGGGGTAATTGAGGAAGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGCTGTGGAAGGAGAAGGGACAGATTGCCAGCAGCAGCGATGCCACCGGCTGGAGCCTCGAACCCACCAAGGACG
AGAAGGGCGATTTTTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6QZZ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

54.474

100

0.546
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50.286

92.348

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.888

89.446

0.375
  pilT Legionella pneumophila strain ERS1305867

42.515

88.127

0.375
  pilT Legionella pneumophila strain Lp02

42.515

88.127

0.375
  pilT Pseudomonas aeruginosa PAK

41.279

90.765

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.698

90.765

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.742

87.863

0.367
  pilT Vibrio cholerae strain A1552

41.742

87.863

0.367
  pilT Acinetobacter baumannii strain A118

41.617

88.127

0.367
  pilT Acinetobacter baumannii D1279779

41.617

88.127

0.367
  pilT Acinetobacter nosocomialis M2

41.617

88.127

0.367

Multiple sequence alignment