Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BCT18_RS01335 Genome accession   NZ_CP170034
Coordinates   299334..300563 (+) Length   409 a.a.
NCBI ID   WP_016791284.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.222.54.F11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 294334..305563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCT18_RS01315 (BCT18_01315) ampD 295385..295984 (-) 600 WP_016790757.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCT18_RS01320 (BCT18_01320) nadC 296068..296958 (+) 891 WP_016790034.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCT18_RS01325 (BCT18_01325) pilA 297213..297632 (+) 420 WP_016790035.1 pilin Machinery gene
  BCT18_RS01330 (BCT18_01330) pilB 297633..299318 (+) 1686 WP_016790036.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCT18_RS01335 (BCT18_01335) pilC 299334..300563 (+) 1230 WP_016791284.1 type II secretion system F family protein Machinery gene
  BCT18_RS01340 (BCT18_01340) pilD 300630..301499 (+) 870 WP_102277664.1 prepilin peptidase Machinery gene
  BCT18_RS01345 (BCT18_01345) coaE 301502..302116 (+) 615 WP_010435920.1 dephospho-CoA kinase -
  BCT18_RS01350 (BCT18_01350) zapD 302143..302883 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCT18_RS01355 (BCT18_01355) yacG 302965..303162 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCT18_RS01360 (BCT18_01360) rplS 303458..303811 (-) 354 WP_004735514.1 50S ribosomal protein L19 -
  BCT18_RS01365 (BCT18_01365) trmD 303871..304611 (-) 741 WP_010435912.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  BCT18_RS01370 (BCT18_01370) rimM 304638..305192 (-) 555 WP_016768202.1 ribosome maturation factor RimM -
  BCT18_RS01375 (BCT18_01375) rpsP 305217..305465 (-) 249 WP_004410028.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45093.98 Da        Isoelectric Point: 10.1000

>NTDB_id=1054863 BCT18_RS01335 WP_016791284.1 299334..300563(+) (pilC) [Vibrio cyclitrophicus strain 10N.222.54.F11]
MSSKSKQSQLKSYHWKGINSSGKKVSGQTLALTELEVREKLKEQHIQTKKIKKKSISAVTRLTHRVKAKDITILTRQLAT
MLATGVPIVQAIKLVSDNHRKAEMKSILSHICKGVEAGTPISKAMRTASRHFDDLYTDLVATGELSGNLAQVFERLATYR
EKSEQLKAKVVKALIYPAMVVTVALTVSYLMLTMVIPEFESMFSGFGADLPWFTQQVLDLSHWMQAYSFYTALGIGATSL
VVHQLCQRSYSIRLSASRLVLRFPVLGGVMAKASIAKFSRTLSTSFSSGIPILMSLKTTAKTAGSLHYESAIIEVHRETA
AGMPMYIAMRNTNAFPEMVLQMVMIGEESGNLDDMLNKVASIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLP
IFNLMSVLG

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1054863 BCT18_RS01335 WP_016791284.1 299334..300563(+) (pilC) [Vibrio cyclitrophicus strain 10N.222.54.F11]
ATGAGTAGCAAGTCCAAACAGTCACAATTAAAAAGCTATCACTGGAAAGGCATTAACAGCTCTGGCAAGAAAGTGTCTGG
GCAGACCTTAGCGCTTACTGAATTAGAAGTACGGGAAAAGCTCAAAGAACAGCATATCCAGACTAAGAAAATCAAAAAGA
AAAGTATTTCAGCCGTCACTCGTTTAACCCATAGAGTCAAAGCCAAAGACATCACTATTTTGACGAGACAGCTTGCTACC
ATGCTCGCTACGGGTGTGCCTATCGTGCAAGCCATAAAGTTGGTATCAGATAATCATCGTAAAGCAGAAATGAAATCTAT
TTTATCGCATATCTGCAAAGGTGTAGAAGCCGGAACGCCAATCTCAAAAGCAATGAGAACCGCAAGCCGCCACTTTGACG
ATCTCTACACCGACCTAGTCGCCACCGGAGAGCTTTCCGGCAACCTCGCACAAGTCTTTGAACGCTTAGCGACCTATCGT
GAAAAAAGTGAACAGTTAAAAGCTAAGGTGGTGAAAGCACTTATCTATCCAGCAATGGTGGTCACGGTTGCGCTGACCGT
CTCCTATCTCATGCTCACCATGGTCATCCCAGAGTTTGAATCGATGTTTTCAGGCTTTGGTGCCGATCTTCCCTGGTTCA
CCCAACAAGTCCTCGACCTGTCTCATTGGATGCAAGCTTACAGTTTTTATACCGCCCTAGGCATCGGGGCTACCAGCCTG
GTTGTTCATCAACTGTGCCAACGTTCTTACTCGATTCGACTATCAGCCAGCCGCTTAGTGTTACGCTTTCCTGTACTAGG
TGGCGTAATGGCAAAGGCCTCTATCGCCAAGTTCAGCCGCACCTTATCAACCAGCTTTAGTTCTGGTATTCCGATTCTAA
TGAGCCTCAAAACTACAGCCAAAACCGCAGGCAGTCTGCATTATGAATCTGCGATCATCGAAGTACACCGAGAAACAGCT
GCGGGTATGCCGATGTATATTGCAATGCGCAATACCAATGCCTTCCCTGAAATGGTGTTACAGATGGTGATGATTGGTGA
AGAGTCAGGTAACCTTGATGATATGCTGAATAAAGTCGCTTCCATCTATGAGTTTGAAGTCGACAATACCGTCGACAACT
TAGGCAAAATTTTAGAGCCACTGATCATCGTGTTTTTAGGTACAGTTGTGGGTGGGCTTGTTGTCGCGATGTACTTACCA
ATCTTTAATCTTATGAGTGTGTTAGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

79.25

97.8

0.775
  pilC Vibrio campbellii strain DS40M4

73.039

99.756

0.729
  pilC Acinetobacter baylyi ADP1

41.809

100

0.418
  pilC Legionella pneumophila strain ERS1305867

42.145

98.044

0.413
  pilC Acinetobacter baumannii D1279779

41.584

98.778

0.411
  pilC Pseudomonas stutzeri DSM 10701

40.404

96.822

0.391
  pilG Neisseria gonorrhoeae MS11

39.85

97.555

0.389
  pilG Neisseria meningitidis 44/76-A

39.599

97.555

0.386

Multiple sequence alignment