Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BCU99_RS12690 Genome accession   NZ_CP170015
Coordinates   2866231..2867460 (+) Length   409 a.a.
NCBI ID   WP_016793487.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.52.F6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2861231..2872460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU99_RS12670 (BCU99_12670) ampD 2862233..2862832 (-) 600 WP_016791286.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCU99_RS12675 (BCU99_12675) nadC 2862916..2863806 (+) 891 WP_016768207.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCU99_RS12680 (BCU99_12680) - 2864058..2864531 (+) 474 WP_016768206.1 pilin -
  BCU99_RS12685 (BCU99_12685) pilB 2864531..2866216 (+) 1686 WP_016768205.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCU99_RS12690 (BCU99_12690) pilC 2866231..2867460 (+) 1230 WP_016793487.1 type II secretion system F family protein Machinery gene
  BCU99_RS12695 (BCU99_12695) pilD 2867527..2868396 (+) 870 WP_016793488.1 prepilin peptidase Machinery gene
  BCU99_RS12700 (BCU99_12700) coaE 2868399..2869013 (+) 615 WP_065099652.1 dephospho-CoA kinase -
  BCU99_RS12705 (BCU99_12705) zapD 2869040..2869780 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCU99_RS12710 (BCU99_12710) yacG 2869862..2870059 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCU99_RS12715 (BCU99_12715) rplS 2870320..2870673 (-) 354 WP_004735514.1 50S ribosomal protein L19 -
  BCU99_RS12720 (BCU99_12720) trmD 2870733..2871473 (-) 741 WP_010435912.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  BCU99_RS12725 (BCU99_12725) rimM 2871500..2872054 (-) 555 WP_016768202.1 ribosome maturation factor RimM -
  BCU99_RS12730 (BCU99_12730) rpsP 2872079..2872327 (-) 249 WP_004410028.1 30S ribosomal protein S16 -

Sequence

Protein


Download         Length: 409 a.a.        Molecular weight: 45190.12 Da        Isoelectric Point: 10.1000

>NTDB_id=1054564 BCU99_RS12690 WP_016793487.1 2866231..2867460(+) (pilC) [Vibrio cyclitrophicus strain 10N.286.52.F6]
MSSKSKQSQLKNYHWKGINSSGKKVSGQSLALTELEVREKLKEQHIQIKKIKKKSISAITRITHRVKAKDITVLTRQLAT
MLATGVPIVQAIKLVSDNHRKAEMKSILSHICKGVEAGTPISKAMRTASRHFDDLYTDLVATGELSGNLAQVFERLATYR
EKSEQLKAKVVKALIYPAMVVTVALTVSYLMLTMVIPEFESMFSGFGADLPWFTQQVLDLSHWMQAYSFYTALGIGATTL
VVHQLCQRSYSIRLSASRLVLRFPVLGSVMAKASIAKFSRTLSTSFSSGIPILMSLKTTAKTAGNLHYESAIIEVHRETA
AGMPMYIAMRNTNAFPEMVLQMVMIGEESGNLDDMLNKVASIYEFEVDNTVDNLGKILEPLIIVFLGTVVGGLVVAMYLP
IFNLMSVLG

Nucleotide


Download         Length: 1230 bp        

>NTDB_id=1054564 BCU99_RS12690 WP_016793487.1 2866231..2867460(+) (pilC) [Vibrio cyclitrophicus strain 10N.286.52.F6]
ATGAGTAGTAAAAGCAAACAATCACAACTAAAAAACTACCATTGGAAGGGAATCAATAGTTCTGGTAAGAAAGTGTCTGG
GCAGAGCTTAGCGCTCACCGAATTAGAAGTGCGAGAGAAGCTCAAAGAGCAGCATATCCAAATTAAGAAAATCAAAAAGA
AAAGTATCTCCGCAATCACTCGCATAACACACCGAGTCAAAGCTAAGGACATCACCGTTTTAACTCGGCAACTCGCGACT
ATGTTGGCTACGGGTGTGCCTATTGTACAAGCCATCAAGTTGGTATCAGATAATCATCGTAAAGCAGAAATGAAATCTAT
TTTATCGCATATCTGCAAAGGTGTAGAAGCCGGAACGCCAATCTCAAAAGCAATGAGAACCGCAAGCCGCCACTTTGACG
ATCTCTACACCGACCTAGTCGCCACCGGAGAGCTTTCCGGCAACCTCGCACAAGTCTTTGAACGCTTAGCGACATATCGT
GAAAAAAGTGAACAGTTAAAAGCTAAGGTGGTGAAAGCGCTTATCTATCCAGCAATGGTGGTCACGGTTGCGCTGACCGT
CTCCTATCTCATGCTAACCATGGTCATCCCAGAGTTTGAATCGATGTTTTCAGGCTTTGGTGCCGATCTTCCCTGGTTCA
CCCAACAAGTCCTCGACCTGTCTCATTGGATGCAAGCTTACAGTTTTTATACCGCCCTAGGCATCGGGGCTACCACGCTG
GTTGTTCATCAACTGTGCCAACGTTCTTACTCGATTCGACTATCAGCCAGCCGCTTAGTGTTACGCTTTCCTGTACTTGG
TAGCGTAATGGCAAAGGCCTCTATCGCCAAGTTCAGCCGCACCTTATCAACCAGCTTTAGCTCTGGTATTCCCATCTTAA
TGAGCCTCAAAACCACCGCAAAGACTGCAGGTAATCTGCATTATGAATCTGCGATCATCGAAGTACACCGAGAAACAGCC
GCGGGTATGCCGATGTATATTGCAATGCGCAATACCAATGCCTTCCCTGAAATGGTGTTACAGATGGTGATGATTGGTGA
AGAGTCAGGTAACCTTGATGATATGCTGAATAAAGTCGCTTCCATCTATGAGTTTGAAGTCGACAATACCGTCGACAACT
TAGGCAAAATTTTAGAGCCACTGATCATCGTGTTTTTAGGTACGGTTGTGGGTGGGCTTGTTGTCGCGATGTACTTACCA
ATCTTTAATCTTATGAGTGTGTTAGGATAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(75-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

79.5

97.8

0.778
  pilC Vibrio campbellii strain DS40M4

73.892

99.267

0.734
  pilC Acinetobacter baylyi ADP1

41.809

100

0.418
  pilC Acinetobacter baumannii D1279779

41.337

98.778

0.408
  pilC Legionella pneumophila strain ERS1305867

42.065

97.066

0.408
  pilC Pseudomonas stutzeri DSM 10701

40.594

98.778

0.401
  pilG Neisseria gonorrhoeae MS11

40.602

97.555

0.396
  pilG Neisseria meningitidis 44/76-A

40.351

97.555

0.394

Multiple sequence alignment