Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BCU99_RS12685 Genome accession   NZ_CP170015
Coordinates   2864531..2866216 (+) Length   561 a.a.
NCBI ID   WP_016768205.1    Uniprot ID   -
Organism   Vibrio cyclitrophicus strain 10N.286.52.F6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2859531..2871216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCU99_RS12665 (BCU99_12665) pdhR 2860990..2861751 (-) 762 WP_016768209.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BCU99_RS12670 (BCU99_12670) ampD 2862233..2862832 (-) 600 WP_016791286.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BCU99_RS12675 (BCU99_12675) nadC 2862916..2863806 (+) 891 WP_016768207.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BCU99_RS12680 (BCU99_12680) - 2864058..2864531 (+) 474 WP_016768206.1 pilin -
  BCU99_RS12685 (BCU99_12685) pilB 2864531..2866216 (+) 1686 WP_016768205.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BCU99_RS12690 (BCU99_12690) pilC 2866231..2867460 (+) 1230 WP_016793487.1 type II secretion system F family protein Machinery gene
  BCU99_RS12695 (BCU99_12695) pilD 2867527..2868396 (+) 870 WP_016793488.1 prepilin peptidase Machinery gene
  BCU99_RS12700 (BCU99_12700) coaE 2868399..2869013 (+) 615 WP_065099652.1 dephospho-CoA kinase -
  BCU99_RS12705 (BCU99_12705) zapD 2869040..2869780 (+) 741 WP_010435917.1 cell division protein ZapD -
  BCU99_RS12710 (BCU99_12710) yacG 2869862..2870059 (+) 198 WP_010435916.1 DNA gyrase inhibitor YacG -
  BCU99_RS12715 (BCU99_12715) rplS 2870320..2870673 (-) 354 WP_004735514.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61942.68 Da        Isoelectric Point: 5.2025

>NTDB_id=1054563 BCU99_RS12685 WP_016768205.1 2864531..2866216(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.52.F6]
MLTNLPTVLRQAGLLSLTQEQAVAEQVQASGISTPEALLAPDLFSGDSLANNIKTIFGLPLVQLANTDYETLCDQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQVELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVKVSDDEITPIEDLSQDDSPVSRFINQILVDAVRKGASDIHFEPYEEHFRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEAQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTSERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDIVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCNHCKTADDSPDIYLRHSIPNSQT
IYKANSQGCNECNQGYSGRVGIYEVMPFTDKLKTSLINKPNALAIEDLARREGMRTLQESGIDKLLEGTTSYQELQRVLY
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1054563 BCU99_RS12685 WP_016768205.1 2864531..2866216(+) (pilB) [Vibrio cyclitrophicus strain 10N.286.52.F6]
ATGCTAACCAACCTCCCAACCGTTCTACGTCAGGCAGGCTTACTTAGCCTGACTCAAGAACAAGCCGTTGCGGAACAAGT
ACAAGCTTCAGGCATTTCGACACCTGAAGCTTTGCTCGCTCCGGATTTGTTCAGCGGCGATTCCTTAGCAAACAACATTA
AAACCATCTTCGGCTTACCGTTAGTACAACTTGCCAATACGGACTATGAAACCTTATGTGACCAATTAGGGCTTCGCGAA
CTGATTACTAAATATCGTGCTATCCCGATTTCAGTTTCTAGCTCAACTCTCACACTCGCTTCTGCCGACCCTACGGATTT
ACAGGCTGAAGATGACTTCCGCTTTGCGACAGGATTGCAAGTTGAATTGGTTGTCGCAAACTACTCTGAATTAGAAGGTG
CGATACGCAAGCTGTATGGCCGCTCTATTTCCGGGCAAGATTCTAAGCGTAAGGAGATCACCCAAGATGAGTTAGCTAAC
CTCGTGAAGGTATCTGACGACGAAATAACGCCGATTGAAGATCTCAGCCAAGATGACTCTCCTGTGAGTCGCTTTATCAA
TCAGATCTTGGTTGATGCGGTACGTAAAGGCGCATCGGACATCCACTTCGAACCTTATGAAGAACACTTCCGTGTACGCC
TGCGTTGTGATGGCATATTGGTTGAAATCCAACAGCCCGCCTCTCATTTAAGCCGTCGTCTGTCAGCTCGCTTAAAGATC
CTCGCTAAACTCGATATTGCCGAACGTCGCTTGCCTCAAGATGGTCGTATCAAACTGCGTTTAAATGACGAACTGGCAAT
TGATATGCGTGTGTCTACGCTGCCAACATTGTGGGGAGAAAAGATCGTACTGCGATTGCTTGATAGCAGTGCGGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGCTCAAAAAGCCCTCTACCTCAATGCACTAAAGCGCCCGCAAGGGATGATC
TTAATGACCGGGCCAACCGGCAGCGGTAAAACGGTTTCTCTTTATACTGGACTTCGAGTACTCAACACCTCTGAACGCAA
CATCTCAACAGCTGAAGACCCTGTAGAGATTAACCTGTGTGGCATCAATCAGGTACAAGTGACGCCTAAGATTGGCTTTG
GTTTCGCCGAGGCACTACGATCGTTTTTACGACAAGACCCAGACATCGTGATGGTTGGAGAAATCCGAGATTTAGAAACC
GCCGAAATCGCGATCAAAGCCTCCCAAACCGGGCATTTAGTACTTTCAACACTGCACACTAACTCAGCCGCAGAAACCGT
GACCCGGCTTGCTCATATGGGAATCGAGCCCTTCAACCTTGCTTCGTCTTTGAGCTTGATTATTGCTCAGCGTCTTGCGA
GACGCTTGTGTAACCACTGTAAAACCGCAGACGACTCACCAGACATTTATCTGCGCCACTCTATCCCAAACAGCCAAACC
ATCTACAAAGCCAACTCTCAAGGCTGTAATGAATGTAACCAAGGTTACTCAGGTCGAGTGGGTATCTATGAAGTGATGCC
GTTTACCGACAAGCTAAAAACCAGCCTTATCAATAAACCCAATGCGCTAGCGATTGAAGATCTCGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAGTCAGGGATAGATAAACTGCTTGAAGGCACCACCAGCTACCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(11-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

73.582

100

0.74
  pilB Vibrio campbellii strain DS40M4

70.819

100

0.709
  pilB Vibrio parahaemolyticus RIMD 2210633

70.641

100

0.708
  pilB Legionella pneumophila strain ERS1305867

48.101

98.574

0.474
  pilB Acinetobacter baumannii D1279779

50.095

94.296

0.472
  pilB Acinetobacter baylyi ADP1

49.225

91.979

0.453
  pilF Neisseria gonorrhoeae MS11

43.44

100

0.437
  pilB/pilB1 Synechocystis sp. PCC 6803

35.245

100

0.373
  pilF Thermus thermophilus HB27

38.697

93.048

0.36

Multiple sequence alignment