Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEVBK_RS03410 Genome accession   NZ_CP169531
Coordinates   695675..697390 (-) Length   571 a.a.
NCBI ID   WP_375057196.1    Uniprot ID   -
Organism   Zobellella sp. DQSA1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 690675..702390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEVBK_RS03385 (ACEVBK_03385) - 690838..692016 (+) 1179 WP_375057191.1 PLP-dependent aminotransferase family protein -
  ACEVBK_RS03390 (ACEVBK_03390) trmB 692170..692886 (+) 717 WP_375057192.1 tRNA (guanosine(46)-N7)-methyltransferase TrmB -
  ACEVBK_RS03395 (ACEVBK_03395) coaE 692894..693499 (-) 606 WP_375057193.1 dephospho-CoA kinase -
  ACEVBK_RS03400 (ACEVBK_03400) pilD 693496..694350 (-) 855 WP_375057194.1 prepilin peptidase Machinery gene
  ACEVBK_RS03405 (ACEVBK_03405) pilC 694383..695609 (-) 1227 WP_375057195.1 type II secretion system F family protein Machinery gene
  ACEVBK_RS03410 (ACEVBK_03410) pilB 695675..697390 (-) 1716 WP_375057196.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEVBK_RS03415 (ACEVBK_03415) - 697394..697879 (-) 486 WP_375057197.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACEVBK_RS03420 (ACEVBK_03420) nadC 698203..699045 (-) 843 WP_375058506.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ACEVBK_RS03425 (ACEVBK_03425) - 699102..699614 (-) 513 WP_375057198.1 TIGR02281 family clan AA aspartic protease -
  ACEVBK_RS03430 (ACEVBK_03430) ampD 699698..700252 (+) 555 WP_375057199.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ACEVBK_RS03435 (ACEVBK_03435) pdhR 700642..701403 (+) 762 WP_375057200.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62914.21 Da        Isoelectric Point: 5.5290

>NTDB_id=1051811 ACEVBK_RS03410 WP_375057196.1 695675..697390(-) (pilB) [Zobellella sp. DQSA1]
MTLPINSIRSGLARSLAASSLLLPDELDQAQSQARQEGKPLSTVLVEQHLLPSCTLARFCEQEYGLPLVDLNDFEPDQVP
PQYLSMDLIERHHVLPIYMQGKVLYLAMSDPTNVAALEDFSFRFNLVTDALLVEEDKLQQMLTRLQQGGSGGVLDLEHIE
DTDISGLELDDDPAEHDADLGNTDDDAPIVRYINKILLDAVRKEASDLHFEPYERFYRIRFRVDGLLHEIASPPVNLATR
FAARLKVMARLDIAERRLPQDGRIKLKLNRNKAVDMRVNSLPTQWGEKVVIRILDSSGARLDIDQLGYDEHQKQLYLAAL
RKPQGMILVTGPTGSGKTVSLYTGLSILNTVTANISTAEDPIEINLPGINQVQINPKAGLTFAHALRAFLRQDPDIIMVG
EIRDLETAEIAVKAAQTGHLVLSTLHTNSATETLTRLGNMGLPAYNIASSVSLIMAQRLARKLCPHCKTREQVPEDQLLA
LGFDPAQLAKGVELYKAAGCNRCTGGYKGRIGIYEVLPMNERLASLIMEGANSLTLARAAEKDGMVSLRRAALEKARAGI
ISLAEVNRISG

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=1051811 ACEVBK_RS03410 WP_375057196.1 695675..697390(-) (pilB) [Zobellella sp. DQSA1]
ATGACCCTACCCATTAATAGCATTAGAAGCGGTCTGGCCAGAAGCCTGGCCGCTTCCTCCCTTTTATTGCCCGACGAGCT
GGATCAGGCCCAGAGCCAGGCCCGGCAGGAAGGCAAGCCGCTCAGCACCGTGCTGGTGGAGCAGCACCTGCTGCCCAGTT
GCACCCTGGCGCGGTTCTGTGAGCAGGAGTACGGCCTGCCGCTGGTCGACCTGAATGACTTTGAGCCGGACCAGGTACCG
CCGCAGTATCTCAGCATGGACCTGATTGAGCGCCACCATGTGCTCCCCATCTATATGCAGGGCAAGGTGCTGTACCTGGC
CATGTCCGATCCCACCAATGTGGCGGCGCTGGAAGACTTCAGCTTCCGCTTCAACCTGGTGACCGATGCCCTGCTGGTGG
AGGAGGACAAGCTGCAGCAAATGCTGACCCGGCTGCAACAGGGCGGCAGCGGCGGCGTGCTCGACCTGGAACACATAGAG
GATACGGATATTTCCGGGCTGGAGCTGGACGATGACCCGGCCGAGCACGATGCGGATCTGGGCAATACCGACGATGACGC
CCCCATAGTGCGCTATATCAACAAAATCCTGCTGGATGCGGTGCGCAAGGAAGCCTCCGACCTGCACTTTGAACCCTATG
AGCGCTTCTATCGCATCCGTTTCCGGGTAGACGGCCTGCTGCACGAGATTGCCTCGCCACCGGTTAACCTGGCCACCCGT
TTTGCCGCCCGGCTAAAGGTCATGGCCAGGCTGGATATTGCCGAGCGCCGGCTGCCGCAGGACGGCCGCATCAAGCTCAA
GCTGAACCGCAACAAAGCGGTGGACATGCGGGTCAACAGCCTGCCCACCCAGTGGGGAGAGAAAGTCGTTATTCGTATCC
TGGACAGCTCCGGCGCCAGGCTCGACATCGACCAGCTCGGCTACGACGAGCACCAGAAGCAACTGTATCTGGCCGCCCTG
CGCAAGCCCCAGGGCATGATACTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTATCCCTTTATACCGGCCTGAGCAT
CCTCAATACCGTGACCGCCAATATCTCCACCGCCGAAGATCCGATAGAAATCAACCTGCCCGGCATCAACCAGGTACAGA
TCAACCCCAAGGCCGGGCTGACCTTCGCCCATGCCCTGCGGGCCTTTTTACGCCAGGATCCGGACATCATCATGGTGGGG
GAAATTCGCGATCTGGAAACCGCCGAAATCGCGGTAAAGGCCGCCCAGACCGGCCACCTGGTGCTTTCAACCCTGCACAC
CAACTCCGCCACCGAAACCCTGACCCGGCTCGGCAACATGGGGCTGCCGGCCTATAACATCGCCTCTTCCGTTTCCTTGA
TCATGGCCCAGCGACTGGCGCGCAAGCTGTGCCCGCACTGCAAGACCCGGGAGCAGGTGCCGGAGGATCAGTTGCTGGCG
CTGGGTTTTGACCCGGCCCAACTGGCCAAAGGCGTTGAACTGTACAAGGCCGCCGGCTGCAACCGCTGTACCGGCGGATA
CAAGGGGCGCATCGGTATCTACGAAGTTCTGCCGATGAACGAAAGGCTGGCCAGCCTGATCATGGAAGGCGCCAACTCCC
TGACCCTCGCCAGGGCGGCGGAAAAAGACGGCATGGTCAGCCTGCGCCGGGCGGCGCTGGAAAAAGCCAGGGCAGGCATT
ATCAGCCTGGCCGAAGTCAACCGCATCAGCGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-148)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.743

98.949

0.522
  pilB Legionella pneumophila strain ERS1305867

52.575

98.599

0.518
  pilB Acinetobacter baylyi ADP1

52.398

98.599

0.517
  pilB Vibrio cholerae strain A1552

54.029

95.622

0.517
  pilB Vibrio campbellii strain DS40M4

52.477

95.447

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

50.355

98.774

0.497
  pilF Neisseria gonorrhoeae MS11

47.059

98.249

0.462
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.194

95.622

0.375
  pilF Thermus thermophilus HB27

37.108

100

0.373
  pilB/pilB1 Synechocystis sp. PCC 6803

39.163

92.119

0.361

Multiple sequence alignment