Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   ACEPPP_RS18640 Genome accession   NZ_CP169217
Coordinates   4155833..4157053 (+) Length   406 a.a.
NCBI ID   WP_374088858.1    Uniprot ID   -
Organism   Methylomicrobium lacus strain 22M6SE5-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4150833..4162053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPPP_RS18630 (ACEPPP_18630) - 4151209..4154004 (+) 2796 WP_374088856.1 valine--tRNA ligase -
  ACEPPP_RS18635 (ACEPPP_18635) pilB 4154114..4155826 (+) 1713 WP_374088857.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEPPP_RS18640 (ACEPPP_18640) pilC 4155833..4157053 (+) 1221 WP_374088858.1 type II secretion system F family protein Machinery gene
  ACEPPP_RS18645 (ACEPPP_18645) pilD 4157117..4158007 (+) 891 WP_374088859.1 A24 family peptidase Machinery gene
  ACEPPP_RS18650 (ACEPPP_18650) coaE 4158020..4158652 (+) 633 WP_374088860.1 dephospho-CoA kinase -
  ACEPPP_RS18655 (ACEPPP_18655) zapD 4158630..4159400 (+) 771 WP_374088861.1 cell division protein ZapD -
  ACEPPP_RS18660 (ACEPPP_18660) yacG 4159400..4159591 (+) 192 WP_374088862.1 DNA gyrase inhibitor YacG -
  ACEPPP_RS18665 (ACEPPP_18665) - 4159603..4160556 (-) 954 WP_374088863.1 Nudix family hydrolase -
  ACEPPP_RS18670 (ACEPPP_18670) argJ 4160575..4161789 (-) 1215 WP_374088864.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44918.06 Da        Isoelectric Point: 9.0913

>NTDB_id=1049864 ACEPPP_RS18640 WP_374088858.1 4155833..4157053(+) (pilC) [Methylomicrobium lacus strain 22M6SE5-12]
MAQKVEQIDFVWDGVDKAGNKIKNRPISALSETIAKADLKRQGFRVQKIKKKPKPLFKKVQKITPADIAVFARQLATMLG
AGVPLVQSFDIIGKGHENPSMQEMLLSIKASIEGGNTLAQALREKSLYFDELFCNLVEAGEQAGVLEALLDKIATYKEKT
ESIKKKIKKALTYPTAVIVVAVIVTTILLMFVVPVFEELFKGFGADLPAFTQMVINMSNWLRDWWWIVFGSIGGIFYTFF
YFKKRSYAFNHALDRMLLKVPVIGTILNKSAIARFARTLSTMSAAGVPLVEALESVAGACGSIVYAEVVLKMREEVATGQ
RLQFAMQQSNLFPHMMQQMVAIGEESGAMDAMLAKVADFYEEEVDNLIDNLSSLMEPFIMAVLGVLVGGLLVAMYMPIFK
LGEAVS

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1049864 ACEPPP_RS18640 WP_374088858.1 4155833..4157053(+) (pilC) [Methylomicrobium lacus strain 22M6SE5-12]
ATGGCACAAAAAGTTGAACAAATCGATTTTGTCTGGGACGGCGTCGACAAGGCCGGCAACAAAATCAAAAATCGGCCCAT
CTCTGCGCTTAGCGAGACGATCGCCAAAGCCGATCTAAAACGGCAAGGCTTTCGCGTCCAGAAAATCAAGAAAAAACCGA
AACCCTTATTCAAGAAAGTCCAAAAAATTACCCCTGCCGATATTGCGGTGTTTGCGCGGCAACTCGCGACGATGCTCGGA
GCCGGCGTGCCGTTGGTCCAGTCTTTCGATATCATCGGCAAGGGCCACGAGAATCCGAGCATGCAGGAGATGTTGTTGTC
GATCAAGGCCAGCATCGAAGGCGGCAACACGCTGGCGCAAGCTTTGCGCGAAAAGTCGCTGTATTTCGACGAATTGTTCT
GCAACCTGGTCGAAGCGGGCGAGCAGGCCGGGGTTCTGGAGGCCTTGCTCGATAAAATCGCGACTTACAAGGAAAAAACC
GAATCGATCAAGAAGAAGATCAAAAAAGCGTTGACTTATCCGACCGCGGTGATTGTCGTCGCGGTCATCGTGACCACCAT
CCTGCTGATGTTCGTGGTGCCGGTTTTCGAGGAACTCTTCAAGGGTTTCGGTGCGGATTTGCCGGCATTTACCCAGATGG
TGATCAACATGTCGAATTGGCTCAGGGATTGGTGGTGGATCGTCTTCGGCTCGATCGGAGGCATTTTTTACACGTTTTTT
TATTTCAAAAAACGTTCCTATGCGTTTAACCATGCACTGGACCGGATGTTGTTGAAAGTGCCCGTCATTGGGACGATTTT
GAACAAATCCGCGATCGCGCGCTTCGCCAGAACCTTGTCGACGATGTCGGCGGCCGGGGTGCCGCTGGTGGAAGCTTTGG
AGTCGGTAGCCGGCGCTTGTGGTAGTATAGTCTACGCGGAAGTGGTCCTGAAGATGCGTGAAGAAGTCGCCACAGGGCAG
CGTCTGCAATTTGCGATGCAGCAGTCGAATCTGTTTCCGCATATGATGCAGCAAATGGTTGCAATCGGCGAAGAATCCGG
GGCGATGGATGCGATGCTTGCCAAGGTCGCGGATTTTTATGAAGAAGAGGTCGACAATTTGATCGATAATTTAAGCAGCC
TGATGGAGCCCTTTATTATGGCCGTCCTCGGCGTTCTGGTCGGCGGGCTTTTGGTTGCGATGTATATGCCTATCTTCAAG
TTGGGAGAAGCCGTCAGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

61.404

98.276

0.603
  pilC Acinetobacter baumannii D1279779

56.5

98.522

0.557
  pilC Pseudomonas stutzeri DSM 10701

55.665

100

0.557
  pilC Acinetobacter baylyi ADP1

54.279

100

0.547
  pilG Neisseria meningitidis 44/76-A

46

98.522

0.453
  pilG Neisseria gonorrhoeae MS11

45.75

98.522

0.451
  pilC Vibrio cholerae strain A1552

40.355

97.044

0.392
  pilC Vibrio campbellii strain DS40M4

39.594

97.044

0.384

Multiple sequence alignment