Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEPPP_RS18635 Genome accession   NZ_CP169217
Coordinates   4154114..4155826 (+) Length   570 a.a.
NCBI ID   WP_374088857.1    Uniprot ID   -
Organism   Methylomicrobium lacus strain 22M6SE5-12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4149114..4160826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEPPP_RS18620 (ACEPPP_18620) - 4149222..4150709 (+) 1488 WP_374088854.1 leucyl aminopeptidase -
  ACEPPP_RS18625 (ACEPPP_18625) - 4150728..4151156 (+) 429 WP_374088855.1 DNA polymerase III subunit chi -
  ACEPPP_RS18630 (ACEPPP_18630) - 4151209..4154004 (+) 2796 WP_374088856.1 valine--tRNA ligase -
  ACEPPP_RS18635 (ACEPPP_18635) pilB 4154114..4155826 (+) 1713 WP_374088857.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEPPP_RS18640 (ACEPPP_18640) pilC 4155833..4157053 (+) 1221 WP_374088858.1 type II secretion system F family protein Machinery gene
  ACEPPP_RS18645 (ACEPPP_18645) pilD 4157117..4158007 (+) 891 WP_374088859.1 A24 family peptidase Machinery gene
  ACEPPP_RS18650 (ACEPPP_18650) coaE 4158020..4158652 (+) 633 WP_374088860.1 dephospho-CoA kinase -
  ACEPPP_RS18655 (ACEPPP_18655) zapD 4158630..4159400 (+) 771 WP_374088861.1 cell division protein ZapD -
  ACEPPP_RS18660 (ACEPPP_18660) yacG 4159400..4159591 (+) 192 WP_374088862.1 DNA gyrase inhibitor YacG -
  ACEPPP_RS18665 (ACEPPP_18665) - 4159603..4160556 (-) 954 WP_374088863.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63045.76 Da        Isoelectric Point: 6.6643

>NTDB_id=1049863 ACEPPP_RS18635 WP_374088857.1 4154114..4155826(+) (pilB) [Methylomicrobium lacus strain 22M6SE5-12]
MATPTTEFYFSGITKCLIQKGLLSEGEARTHVQEAAKKHISLVSYLVANKLVNGRAIASAVYAEFGVPFFDLDAIDLKTL
PVKLINEKLIRKHQVLPLFARGKTIFIAVSDPTNHQPLDEIKFQTRLNPECIVVEEDKLSKAIEIALEAPDTSMAALLDE
DLDNLAVSSVEEEAARNEANSDVDDAPIVRFVNKILLDSVKKGASDIHFEPYEKNFRIRFRTDGMLHEVASPPSAVATKI
ISRIKVMSRLDIAERRVPQDGRIKLIVSKNRTLDFRVNTCPTLFGEKVVMRLLDPTSAQLGIEKLGFEPDQQKLFLEAIN
RPYGLVLVTGPTGSGKTVSLYTGLNILNTIERNISTAEDPVEITVEGINQVNMNVKAGLTFASALRAFLRQDPDVIMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNDAPQTLNRLMQMGIPAFNIVSSINLIMAQRLARRLCVHCKEPSDYPESVLLST
GFKKEELKTLQVYKAVGCEHCTNGYKGRVGIYQVMTLSDNMRRLILEGGNAMQLAEQAKAEGINDLRASGLNKVRQGVTS
IEEIDRVTKE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=1049863 ACEPPP_RS18635 WP_374088857.1 4154114..4155826(+) (pilB) [Methylomicrobium lacus strain 22M6SE5-12]
ATGGCTACACCGACGACTGAATTTTACTTTAGCGGTATTACCAAGTGCCTGATTCAAAAAGGTTTGCTTTCGGAAGGCGA
GGCACGAACCCATGTGCAGGAAGCGGCGAAGAAACACATTTCCCTCGTCAGTTATCTGGTCGCCAATAAATTGGTAAACG
GCAGAGCGATCGCCTCTGCCGTTTACGCCGAATTTGGCGTACCTTTCTTCGATCTGGATGCGATCGATTTAAAGACGCTG
CCGGTCAAATTGATCAATGAAAAATTGATCCGCAAACATCAAGTCCTGCCTCTGTTCGCGCGCGGCAAGACGATTTTCAT
CGCGGTATCCGATCCGACAAATCATCAGCCGCTTGACGAAATCAAGTTTCAAACTCGGTTGAATCCCGAATGCATCGTGG
TCGAAGAGGATAAGCTGAGCAAGGCGATTGAAATCGCTCTGGAGGCTCCCGACACCTCGATGGCCGCCTTGCTCGACGAA
GATCTCGATAATCTGGCGGTTTCCAGCGTGGAAGAAGAGGCCGCCAGAAACGAAGCGAATTCGGATGTCGATGATGCGCC
GATTGTTCGCTTCGTGAATAAAATCCTGCTCGACTCGGTCAAAAAAGGCGCCTCCGATATTCATTTCGAGCCTTACGAAA
AAAACTTCCGCATCCGTTTCCGCACCGACGGCATGCTGCACGAGGTCGCCAGTCCTCCGTCCGCGGTCGCGACCAAGATC
ATTTCCCGCATCAAGGTCATGTCCAGGCTCGATATCGCCGAACGCCGGGTGCCCCAGGACGGACGGATCAAATTGATCGT
TTCCAAGAATCGTACGCTCGATTTCCGGGTCAATACCTGTCCGACTCTGTTCGGCGAAAAGGTGGTCATGCGTCTTCTCG
ATCCGACCAGCGCGCAATTGGGCATCGAAAAGCTCGGTTTCGAACCCGACCAGCAAAAGCTGTTTCTGGAAGCGATCAAT
CGCCCCTATGGGCTGGTGCTGGTCACAGGGCCCACCGGCAGCGGTAAAACGGTGTCGTTATATACCGGACTGAATATCCT
GAACACGATAGAGCGCAATATATCGACCGCGGAAGATCCGGTCGAAATCACCGTAGAAGGCATCAATCAGGTCAACATGA
ACGTGAAGGCCGGTCTGACCTTCGCCAGCGCTTTGCGCGCCTTTTTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAA
ATTCGTGACCTCGAAACCGCCGAGATTGCGATCAAAGCGGCGCAAACCGGGCACTTGGTGCTTTCGACCCTGCACACGAA
CGATGCGCCGCAAACCTTGAACCGGCTGATGCAGATGGGGATTCCCGCGTTCAATATCGTGTCGTCGATCAATCTGATCA
TGGCGCAGCGACTGGCGCGCCGTTTATGCGTGCATTGCAAGGAGCCCTCCGATTATCCGGAAAGTGTGCTGCTTTCGACC
GGTTTCAAGAAAGAAGAATTGAAAACGCTGCAAGTCTATAAGGCCGTGGGCTGCGAGCATTGTACGAACGGTTATAAGGG
CCGGGTCGGCATCTATCAGGTGATGACGCTCAGCGACAACATGCGTAGGCTGATCCTGGAAGGCGGCAACGCGATGCAGT
TGGCCGAGCAGGCGAAGGCGGAGGGCATCAACGATCTCAGGGCTTCCGGCCTGAATAAAGTCCGCCAGGGCGTCACCAGC
ATTGAAGAAATTGACCGGGTTACCAAGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

56.643

100

0.568
  pilB Acinetobacter baylyi ADP1

53.054

100

0.533
  pilB Acinetobacter baumannii D1279779

52.249

100

0.53
  pilF Neisseria gonorrhoeae MS11

48.746

97.895

0.477
  pilB Vibrio cholerae strain A1552

46.903

99.123

0.465
  pilB Vibrio parahaemolyticus RIMD 2210633

46.892

98.772

0.463
  pilB Vibrio campbellii strain DS40M4

46.085

98.596

0.454
  pilB/pilB1 Synechocystis sp. PCC 6803

36.688

100

0.396
  pilF Thermus thermophilus HB27

38.188

98.772

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.188

98.772

0.377

Multiple sequence alignment