Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   ACEN9T_RS11035 Genome accession   NZ_CP168610
Coordinates   496541..497806 (-) Length   421 a.a.
NCBI ID   WP_252690566.1    Uniprot ID   -
Organism   Ralstonia sp. Ralssp110 strain CT11-110     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 491541..502806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9T_RS11005 (ACEN9T_11010) - 492610..493494 (+) 885 WP_092972366.1 ATP-binding protein -
  ACEN9T_RS11010 (ACEN9T_11015) - 493484..493933 (+) 450 WP_092972981.1 NUDIX domain-containing protein -
  ACEN9T_RS11015 (ACEN9T_11020) - 493926..494129 (-) 204 WP_092972367.1 DNA gyrase inhibitor YacG -
  ACEN9T_RS11020 (ACEN9T_11025) zapD 494138..494896 (-) 759 WP_004633038.1 cell division protein ZapD -
  ACEN9T_RS11025 (ACEN9T_11030) coaE 495032..495646 (-) 615 WP_092972983.1 dephospho-CoA kinase -
  ACEN9T_RS11030 (ACEN9T_11035) pilD 495660..496541 (-) 882 WP_413707731.1 prepilin peptidase Machinery gene
  ACEN9T_RS11035 (ACEN9T_11040) pilG 496541..497806 (-) 1266 WP_252690566.1 type II secretion system F family protein Machinery gene
  ACEN9T_RS11040 (ACEN9T_11045) pilB 497851..499578 (-) 1728 WP_252690568.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN9T_RS11045 (ACEN9T_11050) - 499838..501112 (-) 1275 WP_092972372.1 HlyC/CorC family transporter -
  ACEN9T_RS11055 (ACEN9T_11060) ispB 501404..502333 (-) 930 WP_413708443.1 octaprenyl diphosphate synthase -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46554.92 Da        Isoelectric Point: 10.4189

>NTDB_id=1044603 ACEN9T_RS11035 WP_252690566.1 496541..497806(-) (pilG) [Ralstonia sp. Ralssp110 strain CT11-110]
MATRAPAANRAAAAPARKTAKTKAPTQYIFEWEGKDRKGKIFKGEMRAESITEVNAILRKQGLSITKSKRRRAARGKKIT
PKDIAYFTRQLSTMLKAGVPLLQSVDIIAKGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDTLYCNLIDAGEQGG
ILDALLERLSLYMEKSIALKAQIKSAMIYPISVLTVAFLVTVVLMIFVVPAFKGVFSSFGATLPAPTLVVMGISDFFVQW
WYIIVFAPIIGIVLFMRAFKRSENVQRSTHRWILRMPIFGSIIRKATIARWTRTLATMFAAGTPLVESMESVAGAAGNWI
YHDATIEIQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDSMLLKVAEFYEREVDDAVANISALIEPIIIVILG
VLIGGMVVAMYLPIFKLGQVV

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=1044603 ACEN9T_RS11035 WP_252690566.1 496541..497806(-) (pilG) [Ralstonia sp. Ralssp110 strain CT11-110]
ATGGCCACACGAGCACCCGCCGCGAACCGCGCGGCCGCCGCGCCGGCGCGAAAGACTGCCAAGACAAAGGCGCCCACGCA
GTACATCTTCGAGTGGGAAGGCAAAGATCGCAAAGGCAAGATCTTCAAGGGCGAGATGCGCGCCGAGAGCATCACCGAGG
TGAATGCCATCCTGCGCAAGCAAGGCCTGTCGATCACGAAGTCCAAGCGCCGCCGTGCGGCCCGCGGCAAGAAGATCACG
CCGAAGGACATCGCCTACTTCACGCGCCAGCTCTCGACCATGCTGAAGGCCGGCGTGCCGCTCCTGCAGTCGGTCGACAT
CATCGCGAAGGGCCATGCCAATCCAAACTTCACGCAACTGCTGACGGAAATCCGTGTCGACATTGAGTCGGGCAGCAGCA
TGGCGCAAGCCTTCCGTCGTCATCCGAAGTACTTTGACACGCTGTACTGCAACCTGATCGACGCTGGTGAGCAAGGCGGT
ATTCTCGATGCCCTGCTGGAGCGGCTTTCGCTGTACATGGAGAAGTCGATCGCGCTGAAGGCGCAGATCAAGTCGGCGAT
GATCTATCCGATCTCGGTGCTGACGGTGGCTTTCCTGGTGACGGTGGTGCTGATGATTTTTGTGGTGCCAGCGTTTAAGG
GCGTGTTTTCCAGCTTCGGTGCTACGCTGCCGGCCCCAACACTGGTGGTGATGGGCATCTCCGACTTCTTCGTGCAATGG
TGGTACATCATCGTTTTTGCCCCCATCATCGGCATCGTGCTGTTCATGCGGGCATTCAAGCGCTCGGAGAACGTGCAGCG
CTCCACGCACCGCTGGATTCTGCGCATGCCGATCTTCGGCAGCATTATCCGCAAAGCCACCATCGCCCGCTGGACGCGTA
CGCTGGCCACCATGTTTGCCGCCGGCACGCCGCTCGTGGAATCGATGGAATCGGTGGCTGGCGCCGCCGGCAACTGGATC
TATCACGACGCCACGATCGAGATCCAACAAGCCGTGCGCATCGGTACCAGCCTGACCAACGCCATGCAGGCCACGCACGT
GTTCGACAACATGGTGCTGCAGATGACGCAGATCGGTGAAGAATCCGGTGCGCTCGACAGCATGCTGCTCAAGGTGGCCG
AGTTCTACGAGCGCGAGGTGGACGATGCGGTGGCTAACATCTCGGCGCTGATCGAGCCAATCATTATCGTGATCCTGGGC
GTGCTGATTGGCGGCATGGTGGTGGCAATGTACCTGCCGATCTTCAAGTTGGGTCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(87-210)

Type II secretion system (T2SS), protein F (T2SSF)

(291-413)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

51.629

94.774

0.489
  pilG Neisseria meningitidis 44/76-A

51.378

94.774

0.487
  pilC Pseudomonas stutzeri DSM 10701

50

95.487

0.477
  pilC Legionella pneumophila strain ERS1305867

47.772

95.962

0.458
  pilC Acinetobacter baumannii D1279779

47.174

96.675

0.456
  pilC Acinetobacter baylyi ADP1

46.683

96.675

0.451
  pilC Vibrio campbellii strain DS40M4

39.259

96.2

0.378
  pilC Vibrio cholerae strain A1552

40.05

94.299

0.378

Multiple sequence alignment