Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACEN9T_RS11040 Genome accession   NZ_CP168610
Coordinates   497851..499578 (-) Length   575 a.a.
NCBI ID   WP_252690568.1    Uniprot ID   -
Organism   Ralstonia sp. Ralssp110 strain CT11-110     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 492851..504578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9T_RS11010 (ACEN9T_11015) - 493484..493933 (+) 450 WP_092972981.1 NUDIX domain-containing protein -
  ACEN9T_RS11015 (ACEN9T_11020) - 493926..494129 (-) 204 WP_092972367.1 DNA gyrase inhibitor YacG -
  ACEN9T_RS11020 (ACEN9T_11025) zapD 494138..494896 (-) 759 WP_004633038.1 cell division protein ZapD -
  ACEN9T_RS11025 (ACEN9T_11030) coaE 495032..495646 (-) 615 WP_092972983.1 dephospho-CoA kinase -
  ACEN9T_RS11030 (ACEN9T_11035) pilD 495660..496541 (-) 882 WP_413707731.1 prepilin peptidase Machinery gene
  ACEN9T_RS11035 (ACEN9T_11040) pilG 496541..497806 (-) 1266 WP_252690566.1 type II secretion system F family protein Machinery gene
  ACEN9T_RS11040 (ACEN9T_11045) pilB 497851..499578 (-) 1728 WP_252690568.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACEN9T_RS11045 (ACEN9T_11050) - 499838..501112 (-) 1275 WP_092972372.1 HlyC/CorC family transporter -
  ACEN9T_RS11055 (ACEN9T_11060) ispB 501404..502333 (-) 930 WP_413708443.1 octaprenyl diphosphate synthase -
  ACEN9T_RS11060 (ACEN9T_11065) rplU 502669..502980 (+) 312 WP_004633023.1 50S ribosomal protein L21 -
  ACEN9T_RS11065 (ACEN9T_11070) rpmA 503018..503278 (+) 261 WP_004633021.1 50S ribosomal protein L27 -
  ACEN9T_RS11070 (ACEN9T_11075) obgE 503468..504562 (+) 1095 WP_316671662.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63648.40 Da        Isoelectric Point: 7.1547

>NTDB_id=1044604 ACEN9T_RS11040 WP_252690568.1 497851..499578(-) (pilB) [Ralstonia sp. Ralssp110 strain CT11-110]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVGSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSANKHF
AQLRLLPLGRRENRLILATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPAEIKSLFTKQEATQIEYDPG
AAAATRRTTADGIDDAPVVRFLQKLFTEALLRGASDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDRKDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLLITAQRLARRLCKDCKKPGPLPKETL
LDAGFTEADVDGSWQPYHPVGCETCNGSGYKGRVGIYQVMPITEAIQEIILTHGTALQIAEQARKEGVLSLRESGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1044604 ACEN9T_RS11040 WP_252690568.1 497851..499578(-) (pilB) [Ralstonia sp. Ralssp110 strain CT11-110]
ATGACACTCGGACTCGCTCTAGCGCAGAGCCGGCGGATCGCGCCGACGCTACTCACCCAGCTCGAACAGAGCGCGCGTGA
AAAACGCACGCAATTGATCGACGAACTGGTGGGCAGCGGGATCATGAGTGCGCACGACCTCGCCGTGTTCGTGGCCGGCA
AGTATCAGATGCCGCTGCTCGACTTGGCTCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCAGCCAACAAGCACTTT
GCCCAGTTGCGCCTGTTGCCGCTGGGCCGCCGCGAAAACCGCCTCATCCTCGCCACGTCCGACCCCAGCGATCCGAAACC
GATCGAAGAGCTGCGTCAGAAGATGAACGTGGCAATCGAAACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCGCCAATGAGGCACCGGCCGAGATCAAGTCACTCTTCACCAAGCAGGAAGCCACCCAGATCGAGTACGACCCGGGT
GCCGCTGCCGCCACGCGTCGCACGACCGCCGATGGCATCGACGATGCCCCCGTGGTGCGCTTCCTGCAGAAGCTCTTTAC
CGAGGCCTTGTTGCGCGGCGCGTCGGACTTGCATTTCGAGCCCTTTGAAACGTTTTACCGGGTGCGTTTCCGTATTGACG
GCATCCTCGCGCAGGTCGCGCAGCCGCCGCTGGATATCCGCGACAAGATCGCGACGCGCATCAAGGTGCTGTCGCGCCTG
GATATCTCGGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTGCTGATCACCGTTCCGAAAGACCGCAAGGACAAGGA
CAACAAGGAGACGATCGAGCGCGCCATCGACTTCCGGGTATCGACGCTGCCAACGCTGTTCGGCGAGAAAATCGTGATCC
GGATTCTGGAGTCGTCCACCGAGCGGCTCGATATCGACCAGCTCGGCTACGAGCCGGAACAGAAGCAGTTGCTGCTCGAC
GTGATCAAGCGCCCGTACGGCATGGTGCTGGTGACGGGGCCAACGGGCTCGGGCAAGACCGTGTCGCTGTACACCTTCCT
GAACAGGCTGAATCAGGGCGACATCAATATCTCTACCGCCGAAGATCCGGCGGAAATCCAGTTGCCCGGCATCAACCAAG
TCAACGTGAATGACAAGGCGGGGCTGACCTTTGCGGCGGCGCTGAAGTCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGAGATCTGGAAACGGCCGACATTTCGATCAAGGCCGCGCAGACCGGGCACTTGGTGTTTTCCACGCT
GCACACCAACGATGCCCCGACCACGCTGACTCGCCTGATGAACATGGGGGTGGCGCCGTTCAACATCGCGTCGTCGGTGC
TGCTGATTACAGCGCAGCGTCTGGCACGCCGGTTGTGCAAGGATTGCAAGAAGCCGGGGCCGCTGCCCAAGGAAACACTG
CTCGACGCGGGCTTTACCGAAGCGGACGTCGACGGCTCCTGGCAGCCGTATCACCCGGTCGGTTGCGAAACCTGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAAGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTTACGCACG
GCACGGCGCTGCAGATCGCCGAGCAGGCGCGTAAAGAGGGCGTGCTGTCGCTGCGCGAATCGGGCTTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGCTAGCCACAACGAACCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

52.128

98.087

0.511
  pilF Neisseria gonorrhoeae MS11

54.074

93.913

0.508
  pilB Acinetobacter baylyi ADP1

50.709

98.087

0.497
  pilB Legionella pneumophila strain ERS1305867

46.816

100

0.473
  pilB Vibrio cholerae strain A1552

45.088

99.13

0.447
  pilB Vibrio campbellii strain DS40M4

45.438

95.304

0.433
  pilB Vibrio parahaemolyticus RIMD 2210633

45.521

95.13

0.433
  pilF Thermus thermophilus HB27

39.115

98.261

0.384

Multiple sequence alignment