Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACEN9H_RS25315 Genome accession   NZ_CP168562
Coordinates   5788786..5789976 (-) Length   396 a.a.
NCBI ID   WP_056328022.1    Uniprot ID   A0A7X3G1C9
Organism   Massilia cellulosiltytica strain CT11-92     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5783786..5794976
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEN9H_RS25290 (ACEN9H_25295) coxB 5784370..5785563 (-) 1194 WP_413672508.1 cytochrome c oxidase subunit II -
  ACEN9H_RS25295 (ACEN9H_25300) - 5785966..5786877 (-) 912 WP_413672509.1 methyltransferase domain-containing protein -
  ACEN9H_RS25300 (ACEN9H_25305) - 5787164..5787772 (+) 609 WP_413672510.1 ComF family protein -
  ACEN9H_RS25305 (ACEN9H_25310) trmL 5787822..5788292 (+) 471 WP_056122601.1 tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL -
  ACEN9H_RS25310 (ACEN9H_25315) rfaE2 5788296..5788775 (-) 480 WP_056123581.1 D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase -
  ACEN9H_RS25315 (ACEN9H_25320) pilU 5788786..5789976 (-) 1191 WP_056328022.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACEN9H_RS25320 (ACEN9H_25325) - 5790118..5791062 (+) 945 WP_056122596.1 hydrogen peroxide-inducible genes activator -
  ACEN9H_RS25325 (ACEN9H_25330) - 5791040..5791561 (-) 522 WP_413672511.1 hypothetical protein -
  ACEN9H_RS25330 (ACEN9H_25335) ubiA 5791729..5792583 (+) 855 WP_413672512.1 4-hydroxybenzoate octaprenyltransferase -
  ACEN9H_RS25335 (ACEN9H_25340) - 5792623..5792943 (+) 321 WP_056122587.1 DUF883 family protein -
  ACEN9H_RS25340 (ACEN9H_25345) - 5792943..5793344 (+) 402 WP_413672513.1 phage holin family protein -
  ACEN9H_RS25345 (ACEN9H_25350) - 5793331..5793624 (+) 294 WP_160409660.1 hypothetical protein -
  ACEN9H_RS25350 (ACEN9H_25355) - 5793621..5794256 (-) 636 WP_082577018.1 hypothetical protein -

Sequence

Protein


Download         Length: 396 a.a.        Molecular weight: 43773.10 Da        Isoelectric Point: 6.2886

>NTDB_id=1044566 ACEN9H_RS25315 WP_056328022.1 5788786..5789976(-) (pilU) [Massilia cellulosiltytica strain CT11-92]
MSSTFGPAEAQAYMHKLLTVMHQQGGSDLFISADFPPSMKHQGAMKPLSQQRLTGDITRALSLSLMNERQRTEFEAEMEC
NFAISLPGVCRFRVNVFVQQQSVGMVVRTIASEIPNFEKLDLPEVLKDVVMTKRGLVLVVGGTGSGKSTTLAAMIDYRNS
NSAGHIITVEDPVEYVHKNKSCLITHREVGVDTLSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQTMDRIINFFPEERRNQLLMDLSSNLRAIVSQRLVRTEDGKGRKAAIEILLNTPTIGEMILKGSFQSIKEIMHKS
RELGMCTFDQALFELYNKGYISYDEAIRNADSANGLRLQIKLSGERKAPGEGGGATKPAELSMLIDEPEEEPPAAS

Nucleotide


Download         Length: 1191 bp        

>NTDB_id=1044566 ACEN9H_RS25315 WP_056328022.1 5788786..5789976(-) (pilU) [Massilia cellulosiltytica strain CT11-92]
ATGTCCTCGACCTTCGGCCCGGCGGAAGCCCAGGCCTATATGCACAAGCTGCTCACTGTCATGCACCAGCAGGGCGGCTC
CGACCTGTTCATCTCGGCCGACTTCCCGCCCAGCATGAAGCACCAGGGCGCGATGAAACCCCTGAGCCAGCAGCGCCTGA
CGGGCGACATTACGCGCGCGCTGTCCCTCTCCCTCATGAACGAGCGGCAGCGGACCGAGTTCGAAGCCGAGATGGAGTGC
AACTTCGCGATTTCCCTGCCGGGCGTGTGCCGGTTCCGCGTGAACGTGTTCGTGCAGCAGCAGAGCGTAGGCATGGTCGT
GCGGACCATTGCATCGGAAATCCCGAACTTCGAAAAACTCGACCTGCCCGAGGTCTTGAAAGATGTCGTGATGACGAAGC
GGGGCCTCGTCCTCGTCGTCGGCGGCACGGGTTCCGGCAAGTCGACGACGCTGGCGGCGATGATCGATTACCGCAACAGC
AACTCGGCCGGCCACATCATCACGGTCGAGGATCCCGTCGAATACGTTCACAAGAACAAGAGCTGCCTGATCACGCACCG
CGAGGTGGGCGTCGACACGCTGTCGTGGCACAACGCGCTGAAGAACACGTTGCGCCAGGCGCCGGACGTGATCCTGATCG
GTGAAATCCGCGACACGGAAACGATGGAACACGCGATTGCGTTCGCCGAAACAGGTCATTTGTGCCTCGGCACGCTGCAC
GCGAACAATGCGAACCAGACGATGGACCGCATCATCAACTTCTTCCCGGAAGAGCGCAGGAATCAATTGCTGATGGACTT
GTCGTCGAACCTGCGTGCCATCGTCTCGCAGCGCCTGGTCCGCACGGAAGACGGCAAGGGGCGCAAGGCGGCCATCGAGA
TCCTGCTGAACACGCCGACGATCGGCGAGATGATCCTGAAAGGCAGCTTCCAGAGCATCAAGGAGATCATGCACAAGTCG
CGCGAGCTGGGCATGTGCACGTTCGACCAGGCGTTGTTCGAGCTCTACAACAAGGGTTACATCAGTTACGACGAAGCGAT
CCGCAACGCCGATTCCGCGAACGGCCTGCGCCTGCAGATCAAGCTGTCGGGCGAGAGAAAAGCGCCGGGCGAGGGCGGCG
GCGCCACGAAGCCGGCCGAGCTGTCGATGCTGATCGACGAGCCGGAAGAGGAACCGCCGGCCGCAAGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(123-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7X3G1C9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.714

88.384

0.528
  pilU Acinetobacter baylyi ADP1

52.561

93.687

0.492
  pilU Vibrio cholerae strain A1552

47.733

94.697

0.452
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

91.919

0.394
  pilT Pseudomonas aeruginosa PAK

43.881

84.596

0.371
  pilT Legionella pneumophila strain ERS1305867

44.648

82.576

0.369
  pilT Legionella pneumophila strain Lp02

44.648

82.576

0.369
  pilT Pseudomonas stutzeri DSM 10701

43.284

84.596

0.366
  pilT Acinetobacter baylyi ADP1

43.505

83.586

0.364

Multiple sequence alignment