Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACDH70_RS19825 Genome accession   NZ_CP167865
Coordinates   4652912..4654042 (+) Length   376 a.a.
NCBI ID   WP_029218821.1    Uniprot ID   A0A2S7CDA2
Organism   Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:1939     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4647912..4659042
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDH70_RS19800 (ACDH70_19800) - 4648129..4648710 (-) 582 WP_228962840.1 M23 family metallopeptidase -
  ACDH70_RS19805 (ACDH70_19805) - 4648861..4649283 (-) 423 WP_104543508.1 HU family DNA-binding protein -
  ACDH70_RS19810 (ACDH70_19810) proC 4649828..4650679 (-) 852 WP_228962841.1 pyrroline-5-carboxylate reductase -
  ACDH70_RS19815 (ACDH70_19815) - 4650728..4651420 (-) 693 WP_228962842.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACDH70_RS19820 (ACDH70_19820) pilT 4651762..4652799 (+) 1038 WP_104543511.1 type IV pilus twitching motility protein PilT Machinery gene
  ACDH70_RS19825 (ACDH70_19825) pilU 4652912..4654042 (+) 1131 WP_029218821.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACDH70_RS19830 (ACDH70_19830) - 4654054..4654314 (+) 261 WP_228962843.1 hypothetical protein -
  ACDH70_RS19835 (ACDH70_19835) - 4654363..4654632 (+) 270 WP_228962844.1 hypothetical protein -
  ACDH70_RS19840 (ACDH70_19840) - 4654907..4655581 (+) 675 WP_228962871.1 YitT family protein -
  ACDH70_RS19845 (ACDH70_19845) - 4655711..4656250 (-) 540 WP_228962845.1 DNA-3-methyladenine glycosylase I -
  ACDH70_RS19850 (ACDH70_19850) - 4656247..4658100 (-) 1854 WP_228962846.1 DUF4153 domain-containing protein -
  ACDH70_RS19855 (ACDH70_19855) - 4658179..4658745 (+) 567 WP_104543519.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41980.26 Da        Isoelectric Point: 6.8287

>NTDB_id=1040015 ACDH70_RS19825 WP_029218821.1 4652912..4654042(+) (pilU) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:1939]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1040015 ACDH70_RS19825 WP_029218821.1 4652912..4654042(+) (pilU) [Xanthomonas axonopodis pv. poinsettiicola strain NCPPB:1939]
ATGAGCACCATCGACTTCACTTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGATCTGTTCATCACCTCCGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACGCAGACGCCGTTGACCGCGCAGCAGAGCCGCGACCTGG
TGTTGAACGTGATGACACCCTCGCAGCGCGAGGAGTTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTCCGTGTCAGCTGCTTCTACCAGCGTAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCACCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAGGGCA
TGGACCATGCCATTGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGATTTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGGCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCACTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGTGAAATCAGCTACGAGGACGCCTTGCGCTACGCCGATTCGCAGAACGA
GGTGCGTTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCCTGGCGCAGGGGCTGGATGGCGTGGAGATCGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7CDA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.302

97.606

0.657
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment