Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCQ12_RS13545 Genome accession   NZ_CP167863
Coordinates   3196991..3198121 (-) Length   376 a.a.
NCBI ID   WP_016902355.1    Uniprot ID   -
Organism   Xanthomonas sp. NCPPB 1068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3191991..3203121
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ12_RS13515 (ACCQ12_13515) - 3192206..3193153 (-) 948 WP_372381961.1 aspartate carbamoyltransferase catalytic subunit -
  ACCQ12_RS13520 (ACCQ12_13520) ruvX 3193167..3193634 (-) 468 WP_039404802.1 Holliday junction resolvase RuvX -
  ACCQ12_RS13525 (ACCQ12_13525) - 3193627..3194193 (-) 567 WP_039404799.1 YqgE/AlgH family protein -
  ACCQ12_RS13530 (ACCQ12_13530) - 3194502..3195068 (+) 567 WP_372381962.1 DNA-3-methyladenine glycosylase I -
  ACCQ12_RS13535 (ACCQ12_13535) - 3195105..3196004 (+) 900 WP_355604317.1 DUF72 domain-containing protein -
  ACCQ12_RS13540 (ACCQ12_13540) - 3196017..3196691 (-) 675 WP_372383617.1 YitT family protein -
  ACCQ12_RS13545 (ACCQ12_13545) pilU 3196991..3198121 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCQ12_RS13550 (ACCQ12_13550) pilT 3198244..3199281 (-) 1038 WP_104543511.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCQ12_RS13555 (ACCQ12_13555) - 3199609..3200301 (+) 693 WP_355581652.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCQ12_RS13560 (ACCQ12_13560) proC 3200345..3201199 (+) 855 WP_372381963.1 pyrroline-5-carboxylate reductase -
  ACCQ12_RS13565 (ACCQ12_13565) - 3201756..3202175 (+) 420 WP_039404742.1 HU family DNA-binding protein -
  ACCQ12_RS13570 (ACCQ12_13570) - 3202326..3202910 (+) 585 WP_355604323.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41966.24 Da        Isoelectric Point: 6.8287

>NTDB_id=1039967 ACCQ12_RS13545 WP_016902355.1 3196991..3198121(-) (pilU) [Xanthomonas sp. NCPPB 1068]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039967 ACCQ12_RS13545 WP_016902355.1 3196991..3198121(-) (pilU) [Xanthomonas sp. NCPPB 1068]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCCGGGAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACGCAGACACCGTTGACCGCGCAACAAAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTATCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGTG
CCACCGGTACCGGTAAATCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTGGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGATCACGCCGTGGCCTTTGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCAAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGTCGCGGCCGGCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTACGAAGACGCCTTGCGCTACGCCGATTCGCAAAACGA
GGTGCGTTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCCTGGCCCAGGGCCTGGATGGCGTGGAGATCGCCG
AAGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.297

94.947

0.402
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment