Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCQ10_RS14430 Genome accession   NZ_CP167862
Coordinates   3378343..3379473 (-) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas sp. NCPPB 1325     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3373343..3384473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ10_RS14400 (ACCQ10_14400) - 3373546..3374493 (-) 948 WP_039404804.1 aspartate carbamoyltransferase catalytic subunit -
  ACCQ10_RS14405 (ACCQ10_14405) ruvX 3374507..3374974 (-) 468 WP_039404802.1 Holliday junction resolvase RuvX -
  ACCQ10_RS14410 (ACCQ10_14410) - 3374967..3375533 (-) 567 WP_039404799.1 YqgE/AlgH family protein -
  ACCQ10_RS14415 (ACCQ10_14415) - 3375842..3376408 (+) 567 WP_372361070.1 DNA-3-methyladenine glycosylase I -
  ACCQ10_RS14420 (ACCQ10_14420) - 3376445..3377344 (+) 900 WP_355604317.1 DUF72 domain-containing protein -
  ACCQ10_RS14425 (ACCQ10_14425) - 3377357..3378031 (-) 675 WP_039566430.1 YitT family protein -
  ACCQ10_RS14430 (ACCQ10_14430) pilU 3378343..3379473 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCQ10_RS14435 (ACCQ10_14435) pilT 3379634..3380671 (-) 1038 WP_104543511.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCQ10_RS14440 (ACCQ10_14440) - 3380998..3381690 (+) 693 WP_184384946.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCQ10_RS14445 (ACCQ10_14445) proC 3381733..3382587 (+) 855 WP_104579947.1 pyrroline-5-carboxylate reductase -
  ACCQ10_RS14450 (ACCQ10_14450) - 3383143..3383562 (+) 420 WP_039404742.1 HU family DNA-binding protein -
  ACCQ10_RS14455 (ACCQ10_14455) - 3383695..3384297 (+) 603 WP_052210287.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=1039949 ACCQ10_RS14430 WP_005990431.1 3378343..3379473(-) (pilU) [Xanthomonas sp. NCPPB 1325]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039949 ACCQ10_RS14430 WP_005990431.1 3378343..3379473(-) (pilU) [Xanthomonas sp. NCPPB 1325]
ATGAGCACCATTGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCCGGGAT
GCCGCCGGCGATCAAGGTGCACGGCAAGATCAGCCCGATCACGCAGACACCGTTGACCGCGCAACAAAGCCGCGACCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACAAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGCAAATCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTGGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATTGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCTATCGCTTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGGCGCAACCAGCTGTTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGATGGTCGCGGCCGGCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATTCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCAGCTACGAAGACGCCTTGCGCTACGCCGATTCGCAGAACGA
GGTGCGTTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCTTGTCGCAGGGCCTGGATGGCGTGGAGATTGCCG
AAGTTCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment