Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ACCQ05_RS07110 Genome accession   NZ_CP167846
Coordinates   1685748..1686878 (+) Length   376 a.a.
NCBI ID   WP_029218821.1    Uniprot ID   A0A2S7CDA2
Organism   Xanthomonas sp. NCPPB 3582     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1680748..1691878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACCQ05_RS07085 (ACCQ05_07085) - 1680973..1681554 (-) 582 WP_372393347.1 M23 family metallopeptidase -
  ACCQ05_RS07090 (ACCQ05_07090) - 1681704..1682126 (-) 423 WP_104543508.1 HU family DNA-binding protein -
  ACCQ05_RS07095 (ACCQ05_07095) proC 1682671..1683522 (-) 852 WP_372393348.1 pyrroline-5-carboxylate reductase -
  ACCQ05_RS07100 (ACCQ05_07100) - 1683571..1684263 (-) 693 WP_228962842.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACCQ05_RS07105 (ACCQ05_07105) pilT 1684605..1685642 (+) 1038 WP_372393349.1 type IV pilus twitching motility protein PilT Machinery gene
  ACCQ05_RS07110 (ACCQ05_07110) pilU 1685748..1686878 (+) 1131 WP_029218821.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ACCQ05_RS07115 (ACCQ05_07115) - 1686875..1687468 (+) 594 WP_372393351.1 MFS transporter permease -
  ACCQ05_RS07120 (ACCQ05_07120) - 1687745..1688419 (+) 675 WP_228962871.1 YitT family protein -
  ACCQ05_RS07125 (ACCQ05_07125) - 1688549..1689088 (-) 540 WP_228962845.1 DNA-3-methyladenine glycosylase I -
  ACCQ05_RS07130 (ACCQ05_07130) - 1689085..1690938 (-) 1854 WP_372393352.1 DUF4153 domain-containing protein -
  ACCQ05_RS07135 (ACCQ05_07135) - 1691017..1691583 (+) 567 WP_372393353.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41980.26 Da        Isoelectric Point: 6.8287

>NTDB_id=1039798 ACCQ05_RS07110 WP_029218821.1 1685748..1686878(+) (pilU) [Xanthomonas sp. NCPPB 3582]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039798 ACCQ05_RS07110 WP_029218821.1 1685748..1686878(+) (pilU) [Xanthomonas sp. NCPPB 3582]
ATGAGCACCATCGACTTCACTTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGATCTGTTCATCACCTCCGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACGCAGACGCCGTTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAGTTCGAAAAGACCCACGAGTGCAATTTCGCCATCGGCGTGTCCGGG
GTCGGGCGCTTCCGTGTCAGCTGCTTCTACCAGCGTAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACACGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAGGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAAAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAACCAGCTGCTGATGGATTTGTCGCTCAACCTCAAGGGCGTGGT
CGCGCAGCAGCTGATTCCGACCCCGGACGGGCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCACTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTCTACCAGGCCGGTGAAATCAGCTACGAGGACGCCTTGCGCTACGCCGATTCGCAGAACGA
GGTGCGTTTGCGCATCAAGCTGTCGCAGGGCGGCGATGCCAAGACCCTGGCGCAGGGGCTGGATGGCGTGGAGATCGCCG
AGGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7CDA2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.302

97.606

0.657
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment