Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABQ179_RS07435 Genome accession   NZ_CP167817
Coordinates   1748748..1749878 (+) Length   376 a.a.
NCBI ID   WP_104614551.1    Uniprot ID   A0A2S7C9D4
Organism   Xanthomonas dyei strain WHRI 5755     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1743748..1754878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQ179_RS07410 (ABQ179_007410) - 1743973..1744581 (-) 609 WP_372469513.1 M23 family metallopeptidase -
  ABQ179_RS07415 (ABQ179_007415) - 1744705..1745127 (-) 423 WP_104614547.1 HU family DNA-binding protein -
  ABQ179_RS07420 (ABQ179_007420) proC 1745655..1746509 (-) 855 WP_349811434.1 pyrroline-5-carboxylate reductase -
  ABQ179_RS07425 (ABQ179_007425) - 1746564..1747256 (-) 693 WP_104614549.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABQ179_RS07430 (ABQ179_007430) pilT 1747583..1748620 (+) 1038 WP_104614550.1 type IV pilus twitching motility protein PilT Machinery gene
  ABQ179_RS07435 (ABQ179_007435) pilU 1748748..1749878 (+) 1131 WP_104614551.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABQ179_RS07440 (ABQ179_007440) - 1750150..1750824 (+) 675 WP_228323207.1 YitT family protein -
  ABQ179_RS07445 (ABQ179_007445) - 1751260..1752396 (-) 1137 WP_372469514.1 hypothetical protein -
  ABQ179_RS07450 (ABQ179_007450) - 1752486..1753025 (-) 540 WP_349811436.1 DNA-3-methyladenine glycosylase I -
  ABQ179_RS07455 (ABQ179_007455) - 1753022..1754866 (-) 1845 WP_104614554.1 DUF4153 domain-containing protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 42021.29 Da        Isoelectric Point: 6.7684

>NTDB_id=1039552 ABQ179_RS07435 WP_104614551.1 1748748..1749878(+) (pilU) [Xanthomonas dyei strain WHRI 5755]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1039552 ABQ179_RS07435 WP_104614551.1 1748748..1749878(+) (pilU) [Xanthomonas dyei strain WHRI 5755]
ATGAGTACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGATCTGTTCATCACCTCCGGCAT
GCCGCCGGCGATCAAGGTAAACGGCAAGATCAGCCCGATCACGCAGACGCCGTTGACCGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCGTCGCAGCGCGAAGAGTTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTCGGGCGTTTCCGTGTGAGCTGCTTCTATCAGCGTAATCAGGTGGGCATGGTGCTGCGTCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATTTTCGTGGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAAGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGTGAGGTGCGCACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGTCACCTGGTGCTGTGCACGCTGCACGCCAACAATGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGACCGCCGCAATCAGCTGCTGATGGATCTGTCGCTGAACCTCAAGGGTGTGGT
GGCGCAGCAACTGATTCCGACCCCGGATGGGCGCAGCCGCCGCGTGGCGATGGAGATCATGCTCGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTGTATCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGCTATGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAGCTCTCGCAGGGCGGCGATGCCAAGACCCTGTCGCAGGGGATGGATGGTGTGGAGATTGCCG
AAGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S7C9D4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.302

97.606

0.657
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.737

94.947

0.396
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364

Multiple sequence alignment