Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AB9N64_RS22290 Genome accession   NZ_CP166962
Coordinates   4836231..4837439 (-) Length   402 a.a.
NCBI ID   WP_019816978.1    Uniprot ID   A0A0W0N6L2
Organism   Pseudomonas sp. KT_2_4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4831231..4842439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9N64_RS22250 (AB9N64_22245) - 4831239..4832537 (-) 1299 WP_014716868.1 NAD(P)/FAD-dependent oxidoreductase -
  AB9N64_RS22255 (AB9N64_22250) - 4832598..4832771 (-) 174 WP_014716869.1 DUF3094 family protein -
  AB9N64_RS22260 (AB9N64_22255) - 4832826..4833455 (-) 630 WP_003188431.1 DUF1780 domain-containing protein -
  AB9N64_RS22265 (AB9N64_22260) - 4833585..4834274 (+) 690 WP_038448869.1 energy-coupling factor ABC transporter permease -
  AB9N64_RS22270 (AB9N64_22265) - 4834339..4834554 (+) 216 WP_003171680.1 hypothetical protein -
  AB9N64_RS22275 (AB9N64_22270) yacG 4834551..4834751 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  AB9N64_RS22280 (AB9N64_22275) coaE 4834748..4835371 (-) 624 WP_032903992.1 dephospho-CoA kinase -
  AB9N64_RS22285 (AB9N64_22280) pilD 4835368..4836234 (-) 867 WP_371359664.1 A24 family peptidase Machinery gene
  AB9N64_RS22290 (AB9N64_22285) pilC 4836231..4837439 (-) 1209 WP_019816978.1 type II secretion system F family protein Machinery gene
  AB9N64_RS22295 (AB9N64_22290) - 4837641..4838054 (+) 414 WP_014716874.1 pilin -
  AB9N64_RS22305 (AB9N64_22300) - 4838344..4838649 (+) 306 WP_014716875.1 DUF6388 family protein -
  AB9N64_RS22310 (AB9N64_22305) nadC 4838699..4839547 (-) 849 WP_014716876.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AB9N64_RS22315 (AB9N64_22310) - 4839794..4841941 (+) 2148 WP_019816976.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43821.43 Da        Isoelectric Point: 10.2753

>NTDB_id=1036369 AB9N64_RS22290 WP_019816978.1 4836231..4837439(-) (pilC) [Pseudomonas sp. KT_2_4]
MNDTSTIYAWEGINRKGRRVSGQIAGHNLALIKLQLRQQGICPERVRKKSYGLHIFKTRITPADIALFTRQLATLLKAGI
PLLHAFDIISEGVENPQLRELVKALKQHIASGHPLAAALRQYPRHFDELYCNLVAAGEMAGALETLLERVAIHLEKSQRL
KTRIKKAMTYPFAVLVIATGVSTVLLIHVVPQFQNLFAGVNGQLPGFTLGVIALSELLQQAWWMLGLGVGAALVVGRHAY
RTYPGFRHWWAAGLLKVPMAGKLLHKSAVARYARTLSTTFAAGVPLVQALGSVAGAVGNGPFKQAIERMRHDVSTGMQLN
QSMHNSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYESDVDTLVDNLTSLMEPLIMVVLGGIVGALVVAMYLPVFQLGT
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=1036369 AB9N64_RS22290 WP_019816978.1 4836231..4837439(-) (pilC) [Pseudomonas sp. KT_2_4]
ATGAACGACACGTCGACAATTTATGCCTGGGAAGGCATCAACCGCAAAGGGCGCCGGGTGTCCGGGCAAATCGCCGGCCA
TAACCTTGCGCTGATCAAGCTACAACTGCGCCAGCAAGGGATCTGCCCTGAGCGTGTGCGCAAGAAATCTTATGGTTTAC
ACATCTTCAAGACGCGAATAACGCCTGCAGATATCGCCTTGTTCACTCGCCAGTTGGCCACCTTGCTCAAAGCCGGCATC
CCGCTGCTGCACGCCTTCGACATCATCAGCGAAGGCGTCGAAAACCCACAACTGCGCGAACTGGTCAAGGCGTTGAAACA
GCATATTGCCAGCGGCCACCCGTTGGCCGCTGCCCTGCGTCAATATCCCCGCCACTTCGATGAGCTGTACTGCAATCTGG
TGGCGGCTGGCGAAATGGCCGGCGCCCTGGAAACCCTGCTGGAGCGTGTGGCGATTCATTTGGAAAAAAGCCAACGGCTC
AAAACCAGGATCAAGAAAGCCATGACCTACCCTTTCGCGGTACTGGTAATTGCCACAGGGGTGAGCACAGTGCTGTTGAT
CCATGTGGTGCCGCAATTCCAGAACTTGTTCGCCGGGGTCAACGGCCAATTGCCGGGGTTTACCCTGGGGGTCATCGCGC
TGTCGGAACTGCTGCAACAGGCGTGGTGGATGCTGGGGCTGGGTGTGGGCGCGGCGCTGGTTGTGGGGCGCCACGCCTAC
CGTACTTACCCGGGGTTTCGCCATTGGTGGGCAGCAGGTTTGTTGAAAGTGCCCATGGCAGGCAAACTGCTGCACAAATC
TGCGGTCGCCCGCTACGCTCGTACACTTTCCACCACCTTTGCGGCGGGCGTCCCTCTGGTACAGGCATTGGGTTCGGTGG
CCGGCGCCGTCGGCAATGGCCCCTTCAAACAGGCAATCGAACGTATGCGCCACGATGTATCCACAGGCATGCAGTTGAAT
CAATCGATGCATAACAGCGGCCTGTTCCCAGGCATGGCGATCCAGATGACCGCAATCGGGGAAGAGTCCGGCACCCTGGA
CCGCATGCTGGAAAAAGTCGCGAACCACTACGAGTCAGACGTGGATACCTTGGTCGACAACCTCACCAGCCTGATGGAGC
CGCTGATCATGGTCGTATTGGGAGGGATTGTCGGCGCGCTGGTAGTGGCCATGTACCTGCCGGTGTTCCAATTGGGGACG
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0N6L2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.035

99.005

0.565
  pilC Acinetobacter baylyi ADP1

50.253

98.507

0.495
  pilC Acinetobacter baumannii D1279779

49.873

98.259

0.49
  pilC Legionella pneumophila strain ERS1305867

47.848

98.259

0.47
  pilC Vibrio cholerae strain A1552

39.949

97.761

0.391
  pilG Neisseria gonorrhoeae MS11

39.844

95.522

0.381
  pilC Thermus thermophilus HB27

37.905

99.751

0.378
  pilG Neisseria meningitidis 44/76-A

39.323

95.522

0.376
  pilC Vibrio campbellii strain DS40M4

38.01

97.512

0.371

Multiple sequence alignment