Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB9R83_RS12100 Genome accession   NZ_CP166302
Coordinates   2532742..2533851 (-) Length   369 a.a.
NCBI ID   WP_317074629.1    Uniprot ID   -
Organism   Oceanimonas smirnovii strain OS2020-127     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2527742..2538851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB9R83_RS12080 (AB9R83_12070) brnQ 2528281..2529585 (-) 1305 WP_026344567.1 branched-chain amino acid transport system II carrier protein -
  AB9R83_RS12085 (AB9R83_12075) - 2529750..2530457 (-) 708 WP_395544927.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  AB9R83_RS12090 (AB9R83_12080) srmB 2530528..2531829 (+) 1302 WP_418135413.1 ATP-dependent RNA helicase SrmB -
  AB9R83_RS12095 (AB9R83_12085) yaaA 2531955..2532734 (-) 780 WP_395544926.1 peroxide stress protein YaaA -
  AB9R83_RS12100 (AB9R83_12090) pilU 2532742..2533851 (-) 1110 WP_317074629.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB9R83_RS12105 (AB9R83_12095) pilT 2533868..2534902 (-) 1035 WP_317074630.1 type IV pilus twitching motility protein PilT Machinery gene
  AB9R83_RS12110 (AB9R83_12100) - 2534943..2535635 (+) 693 WP_019933104.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB9R83_RS12115 (AB9R83_12105) proC 2535646..2536470 (+) 825 WP_019933103.1 pyrroline-5-carboxylate reductase -
  AB9R83_RS12120 (AB9R83_12110) - 2536480..2537031 (+) 552 WP_019933102.1 YggT family protein -
  AB9R83_RS12125 (AB9R83_12115) - 2537101..2537514 (+) 414 WP_019933101.1 DUF4426 domain-containing protein -
  AB9R83_RS12130 (AB9R83_12120) rdgB 2537526..2538131 (+) 606 WP_395544924.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41068.83 Da        Isoelectric Point: 5.8398

>NTDB_id=1034120 AB9R83_RS12100 WP_317074629.1 2532742..2533851(-) (pilU) [Oceanimonas smirnovii strain OS2020-127]
MELASLLSRMCEHKASDMYISVGIAPTIKTQGKLMPLGEQPLTEQETLALVESCMTAENKALFHQQKEANFAIHDDDHGR
FRVSAFWQQDKAGMVLRRIESRIPSFEELYLPETLRDIAMAKRGLVLFVGGTGTGKSTTQAAMIGHRNGHADGHILTIED
PVEFVHQHQRSIITQREVGIDTESFDAALKSSLRQAPDVILIGEIRSEETMGYALSFAETGHLCMATLHANNANQAIDRI
MHLVPENKHRQLLFDLSFNLKAIVAQQLIPTQDGTQRRAAFEILLNTPLIADVIRQGDMHKLKEIMAKSRESGMQTFDQA
LFDLYSQQHIGYTEALAYADSPNDLRLMIKLQGGGGLDSGLFDNVTIES

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1034120 AB9R83_RS12100 WP_317074629.1 2532742..2533851(-) (pilU) [Oceanimonas smirnovii strain OS2020-127]
ATGGAACTGGCGTCATTATTAAGCCGCATGTGCGAGCACAAGGCCTCGGACATGTATATTTCGGTGGGCATTGCGCCCAC
CATCAAAACCCAGGGCAAACTCATGCCTCTGGGCGAGCAGCCACTGACTGAGCAGGAAACGCTGGCGCTGGTTGAATCGT
GTATGACGGCGGAAAACAAGGCGCTTTTTCATCAGCAAAAAGAAGCCAACTTTGCCATACACGACGATGATCATGGCCGC
TTTCGGGTCAGCGCTTTCTGGCAGCAAGACAAGGCCGGCATGGTGCTGCGGCGCATTGAAAGCCGGATTCCGTCTTTTGA
GGAGCTATATCTGCCCGAAACCCTGCGCGACATTGCCATGGCCAAGCGCGGTCTGGTGCTGTTTGTGGGCGGCACCGGCA
CCGGTAAGTCCACCACTCAGGCGGCCATGATAGGCCACCGCAACGGCCATGCCGACGGCCATATTCTGACCATTGAAGAT
CCGGTGGAATTTGTCCATCAGCATCAACGTTCCATTATTACTCAGCGGGAAGTGGGCATCGACACCGAATCGTTCGATGC
TGCGCTGAAAAGCTCGTTGCGTCAGGCCCCGGATGTCATCTTGATTGGTGAAATCCGCTCGGAAGAAACCATGGGTTATG
CCCTGTCGTTTGCTGAAACCGGGCATTTGTGCATGGCTACCCTGCATGCCAACAACGCCAATCAGGCCATTGACCGCATT
ATGCACCTGGTGCCGGAAAACAAGCATCGCCAGCTGCTGTTTGATTTGTCGTTCAACCTGAAAGCCATAGTGGCTCAGCA
ATTGATCCCCACTCAGGATGGAACTCAGCGCCGGGCCGCGTTTGAGATTTTGCTGAATACACCGCTCATTGCCGATGTGA
TCCGTCAGGGAGATATGCACAAGCTCAAGGAGATTATGGCCAAATCCCGTGAGTCCGGTATGCAAACCTTTGATCAGGCT
CTGTTTGACCTCTACAGTCAGCAGCATATAGGCTACACCGAAGCACTGGCCTATGCTGACTCTCCCAACGATCTGCGGCT
GATGATCAAGCTGCAGGGCGGCGGCGGGCTCGACTCCGGCCTGTTCGACAATGTAACCATCGAATCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

58.967

99.729

0.588
  pilU Pseudomonas stutzeri DSM 10701

58.974

95.122

0.561
  pilU Acinetobacter baylyi ADP1

58.523

95.393

0.558
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

93.496

0.401
  pilT Neisseria gonorrhoeae MS11

40.979

88.618

0.363
  pilT Neisseria meningitidis 8013

40.979

88.618

0.363
  pilT Acinetobacter baylyi ADP1

39.181

92.683

0.363
  pilT Legionella pneumophila strain Lp02

40.181

89.702

0.36
  pilT Legionella pneumophila strain ERS1305867

40.181

89.702

0.36

Multiple sequence alignment