Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AB8599_RS14645 Genome accession   NZ_CP166194
Coordinates   3266888..3268615 (-) Length   575 a.a.
NCBI ID   WP_407333236.1    Uniprot ID   -
Organism   Enterovibrio sp. 27052020O     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3261888..3273615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8599_RS14620 (AB8599_14625) - 3262274..3262486 (+) 213 WP_407331598.1 DUF2970 domain-containing protein -
  AB8599_RS14625 (AB8599_14630) - 3262511..3263344 (-) 834 WP_407331599.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  AB8599_RS14630 (AB8599_14635) - 3263449..3264960 (-) 1512 WP_407333234.1 AAA family ATPase -
  AB8599_RS14635 (AB8599_14640) aroB 3264991..3266067 (-) 1077 WP_407331600.1 3-dehydroquinate synthase -
  AB8599_RS14640 (AB8599_14645) aroK 3266085..3266603 (-) 519 WP_026023777.1 shikimate kinase AroK -
  AB8599_RS14645 (AB8599_14650) pilQ 3266888..3268615 (-) 1728 WP_407333236.1 type IV pilus secretin PilQ Machinery gene
  AB8599_RS14650 (AB8599_14655) - 3268670..3269188 (-) 519 WP_407331601.1 pilus assembly protein PilP -
  AB8599_RS14655 (AB8599_14660) - 3269185..3269778 (-) 594 WP_407331602.1 type 4a pilus biogenesis protein PilO -
  AB8599_RS14660 (AB8599_14665) - 3269768..3270355 (-) 588 WP_407331603.1 PilN domain-containing protein -
  AB8599_RS14665 (AB8599_14670) pilM 3270339..3271313 (-) 975 WP_407331604.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62427.81 Da        Isoelectric Point: 4.4840

>NTDB_id=1033647 AB8599_RS14645 WP_407333236.1 3266888..3268615(-) (pilQ) [Enterovibrio sp. 27052020O]
MHWLVVFACLLMTPMVSAANQLQNLEFVKGENGQTLLTLTFADKSVVTNFDKKDKTLTISAKATQVEDNLIGVRSLESIG
TTATSLDVQTVDDDTQLTLNTKGYFGYDYYQTNNVLTVEIFPFSSLPKSQSNKDKAGKKNANISINFQDISVRSVLQMVA
EHNGFNLVVSDTVNGKLTLRLDDVPWQKALDTILNVKGLDKRQMGNILLVAPKAELDEQERLVLEKRRNERELASLRSEV
LKIKYANAVDLKNMLDGDEDEGISLLTERGSIAVDSRTNALIIRDLADNIDVVKSLIASLDIPVQQVEIEARIVTVDEGT
LEEIGVRWGVSNSNGSFSTGGSIESNLGWNKDIGFDSDSGDFLNISNVGGLESGSGNVDDFLNVNLGAVSPNAASIAFQV
AKLGKDLLLDLELSALQAESRAEIISSPRLLTTNKRAAYIEQGTELPYLEASSSGAAAVSFKKAVLSLSVTPQITPDNKL
VLDLEVTQDKPAGSVKAGTGEAMAINTQRIGTQVLVNDGETVVLGGIYQHEMTVGVDKVPLLGDIPGIGQLFRRDYETMG
KRELLIFVTPRIMTQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1033647 AB8599_RS14645 WP_407333236.1 3266888..3268615(-) (pilQ) [Enterovibrio sp. 27052020O]
ATGCATTGGCTCGTCGTATTCGCATGTTTGCTGATGACACCAATGGTGTCAGCGGCCAACCAGTTACAAAATCTAGAATT
TGTAAAAGGGGAGAATGGCCAAACACTGTTGACCCTGACATTTGCTGATAAATCTGTCGTCACAAATTTTGATAAGAAAG
ACAAAACGTTAACGATAAGTGCCAAAGCAACGCAAGTTGAAGATAACCTGATAGGCGTAAGAAGTCTTGAATCGATTGGT
ACCACAGCAACGTCGCTGGACGTGCAAACGGTCGATGATGATACGCAGTTAACACTCAATACAAAGGGCTATTTTGGTTA
TGACTATTATCAAACCAATAACGTACTGACGGTTGAGATTTTTCCGTTTTCATCCTTACCTAAAAGTCAGTCTAACAAAG
ACAAAGCTGGAAAGAAGAACGCAAATATTTCAATCAACTTCCAGGACATTTCCGTACGCTCTGTGTTGCAAATGGTTGCG
GAGCACAACGGATTTAACTTGGTCGTGTCTGATACAGTTAACGGCAAGCTAACACTTCGACTTGATGATGTTCCGTGGCA
AAAAGCACTCGATACGATTCTGAACGTCAAAGGCTTAGACAAGCGTCAGATGGGTAACATACTGCTGGTAGCCCCAAAGG
CAGAACTTGATGAGCAGGAGCGTTTAGTGCTAGAGAAGCGACGCAACGAGAGAGAGTTGGCATCGCTGCGTTCGGAAGTC
CTGAAAATCAAGTATGCCAATGCGGTTGATCTAAAGAATATGCTCGATGGCGACGAAGATGAAGGTATAAGTCTGTTGAC
TGAACGTGGCAGCATTGCTGTGGATTCGCGCACTAATGCGCTAATTATTAGAGACTTGGCTGACAATATCGATGTGGTGA
AGTCACTCATCGCGTCATTAGATATTCCTGTCCAGCAGGTCGAAATCGAAGCGCGTATTGTCACTGTTGACGAAGGTACT
CTCGAAGAAATTGGTGTGCGCTGGGGAGTGTCGAATAGTAACGGCAGCTTTTCAACTGGGGGGTCGATTGAAAGTAATTT
AGGATGGAACAAAGACATTGGTTTTGACTCAGACAGTGGAGATTTTCTGAATATCAGTAATGTTGGAGGGCTTGAGTCTG
GCTCTGGAAATGTCGATGACTTCCTTAATGTAAATCTCGGTGCTGTTAGCCCAAACGCAGCAAGCATTGCGTTTCAAGTT
GCCAAGCTGGGTAAAGATTTACTGTTAGATCTTGAGTTGTCCGCCCTTCAAGCCGAGTCCCGTGCCGAGATCATTTCGTC
TCCTCGTCTTCTTACGACCAACAAGCGAGCGGCCTACATCGAGCAAGGGACGGAATTACCGTACTTGGAAGCGTCTTCTA
GTGGTGCTGCTGCTGTGTCATTTAAAAAAGCAGTGTTGAGTCTGTCAGTAACGCCTCAAATCACGCCAGACAACAAATTG
GTGTTGGATTTAGAGGTCACTCAAGATAAACCTGCGGGCTCAGTGAAGGCAGGAACGGGTGAGGCTATGGCTATCAATAC
GCAGCGTATTGGCACTCAAGTTCTGGTGAATGACGGTGAAACTGTCGTGTTAGGCGGTATTTATCAACACGAAATGACGG
TGGGCGTCGATAAAGTTCCCTTACTTGGTGATATTCCCGGGATAGGCCAACTGTTCCGTCGAGATTACGAAACTATGGGT
AAACGAGAGCTTCTTATCTTTGTTACACCAAGGATCATGACACAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(239-306)

Bacterial type II and III secretion system protein (Secretin)

(415-573)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

55.363

100

0.557
  pilQ Vibrio cholerae strain A1552

55.363

100

0.557
  pilQ Vibrio campbellii strain DS40M4

54.13

98.957

0.536
  pilQ Legionella pneumophila strain ERS1305867

40.354

98.261

0.397
  pilQ Legionella pneumophila strain Lp02

40.177

98.261

0.395
  pilQ Acinetobacter baumannii D1279779

36.379

100

0.381
  pilQ Acinetobacter baumannii strain A118

36.379

100

0.381
  pilQ Pseudomonas aeruginosa PAK

38.899

97.913

0.381
  comQ Acinetobacter baylyi ADP1

36.762

100

0.379

Multiple sequence alignment