Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8814_RS07670 Genome accession   NZ_CP166090
Coordinates   1853804..1854934 (+) Length   376 a.a.
NCBI ID   WP_006448884.1    Uniprot ID   A0A0G8P0N2
Organism   Xanthomonas hydrangeae strain NCPPB 4692     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1848804..1859934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8814_RS07645 (AB8814_07645) proC 1849634..1850488 (-) 855 WP_205394204.1 pyrroline-5-carboxylate reductase -
  AB8814_RS07650 (AB8814_07650) - 1850536..1851228 (-) 693 WP_205394205.1 YggS family pyridoxal phosphate-dependent enzyme -
  AB8814_RS07655 (AB8814_07655) - 1851385..1851594 (-) 210 WP_210765971.1 NPP1 family protein -
  AB8814_RS07660 (AB8814_07660) - 1851513..1852103 (-) 591 WP_205394206.1 NPP1 family protein -
  AB8814_RS07665 (AB8814_07665) pilT 1852644..1853681 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  AB8814_RS07670 (AB8814_07670) pilU 1853804..1854934 (+) 1131 WP_006448884.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8814_RS07675 (AB8814_07675) - 1855236..1855910 (+) 675 WP_205394800.1 YitT family protein -
  AB8814_RS07680 (AB8814_07680) - 1856051..1856596 (-) 546 WP_205394207.1 DNA-3-methyladenine glycosylase I -
  AB8814_RS07685 (AB8814_07685) - 1856921..1857487 (+) 567 WP_006448880.1 YqgE/AlgH family protein -
  AB8814_RS07690 (AB8814_07690) ruvX 1857480..1857947 (+) 468 WP_205394208.1 Holliday junction resolvase RuvX -
  AB8814_RS07695 (AB8814_07695) - 1857964..1858911 (+) 948 WP_205394209.1 aspartate carbamoyltransferase catalytic subunit -
  AB8814_RS07700 (AB8814_07700) - 1859113..1859547 (+) 435 WP_006448877.1 OsmC family protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41991.26 Da        Isoelectric Point: 6.7684

>NTDB_id=1033320 AB8814_RS07670 WP_006448884.1 1853804..1854934(+) (pilU) [Xanthomonas hydrangeae strain NCPPB 4692]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAVAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLAQGMDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1033320 AB8814_RS07670 WP_006448884.1 1853804..1854934(+) (pilU) [Xanthomonas hydrangeae strain NCPPB 4692]
ATGAGCACTATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCGGGAAT
GCCGCCGGCGATCAAAGTCAATGGCAAGATCAGCCCGATCACCCAGACACCGCTGACTGCACAGCAAAGTCGCGATCTGG
TGTTGAACGTGATGACGCCATCGCAGCGCGAAGAATTCGAAAAGACGCACGAGTGCAACTTCGCCATCGGCGTGTCAGGG
GTCGGGCGTTTTCGTGTGAGCTGTTTCTATCAGCGCAACCAGGTCGGCATGGTGCTGCGCCGGATCGAAACGCGCATTCC
CACAGTGGAAGAGTTGAGCCTGCCGCCGGTGATCAAGACGCTCGCGATGACCAAGCGCGGGATCATCATTTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGTTACCGCAACCAGAATTCGACCGGGCACATCATCACC
ATCGAAGATCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAG
CTGGGAAAACGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGATCACGCCGTGGCCTTTGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAATGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGATCTGTCGCTCAATCTCAAGGGCGTGGT
CGCGCAGCAATTGATTCCGACGCCGGACGGGCGCAGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACCCCGCTGGTGC
AGGATTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTATCAGGCCGGCGAAATCAGCTACGAAGACGCGCTGCGTTACGCCGACTCGCAGAACGA
AGTGCGCCTGCGCATCAAACTCTCGCAGGGCGGCGACGCCAAGACGCTGGCGCAGGGGATGGATGGTGTGGAGATTGCCG
AAGTTCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0G8P0N2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.667

98.138

0.654
  pilU Acinetobacter baylyi ADP1

64.426

94.947

0.612
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.116

91.489

0.367
  pilT Acinetobacter nosocomialis M2

40.116

91.489

0.367
  pilT Acinetobacter baumannii D1279779

40.116

91.489

0.367
  pilT Pseudomonas aeruginosa PAK

40.896

89.096

0.364

Multiple sequence alignment