Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB4P93_RS08435 Genome accession   NZ_CP162596
Coordinates   1680837..1681946 (-) Length   369 a.a.
NCBI ID   WP_210704959.1    Uniprot ID   -
Organism   Pseudomonas sp. B26140     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1675837..1686946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB4P93_RS08430 (AB4P93_08430) - 1676939..1680706 (-) 3768 WP_210704960.1 hypothetical protein -
  AB4P93_RS08435 (AB4P93_08435) pilU 1680837..1681946 (-) 1110 WP_210704959.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB4P93_RS08440 (AB4P93_08440) - 1682074..1684611 (+) 2538 WP_210704958.1 PAS domain-containing protein -
  AB4P93_RS08445 (AB4P93_08445) - 1684663..1684938 (+) 276 WP_210704957.1 peptidylprolyl isomerase -
  AB4P93_RS08450 (AB4P93_08450) - 1685064..1685996 (-) 933 WP_210704956.1 sugar kinase -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40900.78 Da        Isoelectric Point: 6.9794

>NTDB_id=1027330 AB4P93_RS08435 WP_210704959.1 1680837..1681946(-) (pilU) [Pseudomonas sp. B26140]
MEIDALLKQLASRHGSDLYLSTGAPPSARFEGVLTPLSEQSFKPGEVATLATSLMDAEQRREFDRDLEMNLAISRPGIGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKQGLVLFVGATGSGKSTSLAALIDHRNRNSSGHIVTIED
PIEYIHRHQRSIINQREVGVDTRSFHSALKNTLRQAPDVVLIGEIRDRETMEHALSFAETGHLVLSTLHANNANQALDRI
INMFAEERRSQLLQALGNNLKACISQRLVRTVDGNRRAAVEVLLGTPTVADLVRRCEFGELKAMMEKSAAMGMQTFDSAL
FALVSTGAITEEEALRNADSVNNLKLRLKLIGESNIDRNSTAGEWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1027330 AB4P93_RS08435 WP_210704959.1 1680837..1681946(-) (pilU) [Pseudomonas sp. B26140]
ATGGAAATCGATGCACTGCTCAAACAACTGGCGAGTCGTCATGGTTCGGACCTGTACCTCTCCACGGGCGCACCGCCCAG
CGCACGTTTTGAAGGCGTGCTTACCCCGTTGAGTGAGCAATCATTCAAACCTGGCGAGGTGGCAACGCTAGCCACCTCCC
TCATGGACGCCGAGCAGCGCAGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCGCGCCCCGGCATCGGTCGC
TTCCGGGTCAACATCTTTAAACAGCGCAACGATGTATCCATTGTGATCCGCAACGTCAAACTCGATATTCCGCGTTTCGA
GGATCTCAAACTGCCGCCAGTATTGCTCGAAACCGTGATGCTCAAGCAGGGGCTGGTACTGTTTGTCGGTGCTACCGGTT
CGGGAAAATCAACGTCGCTGGCAGCGCTGATCGATCACCGCAACCGCAACAGCAGCGGGCACATCGTCACCATCGAAGAC
CCGATCGAGTATATCCATCGCCATCAGCGTTCGATCATCAACCAGCGTGAAGTCGGGGTCGACACTCGAAGTTTTCACTC
CGCGCTTAAAAATACCTTGCGCCAGGCGCCGGACGTGGTGCTGATCGGTGAAATTCGCGACCGTGAAACCATGGAACACG
CGTTGTCATTTGCCGAGACAGGGCATCTGGTTTTGTCGACGTTGCACGCTAATAACGCCAATCAGGCGTTGGATCGAATT
ATCAATATGTTCGCAGAAGAGCGCCGGTCGCAGTTGCTACAGGCGCTTGGTAACAACTTGAAAGCATGTATTTCGCAGCG
TCTTGTGCGCACTGTTGATGGAAACCGCAGAGCTGCCGTAGAGGTGTTGCTGGGTACGCCGACCGTAGCCGATCTGGTCC
GACGTTGTGAGTTCGGGGAGCTCAAAGCGATGATGGAAAAATCCGCGGCTATGGGTATGCAGACATTTGACTCAGCCCTG
TTTGCGTTGGTATCGACCGGTGCAATCACTGAGGAAGAGGCGTTAAGGAATGCCGATTCGGTCAACAATCTGAAGTTGCG
CTTGAAGTTGATTGGAGAGAGCAACATTGATCGAAATTCAACGGCGGGAGAGTGGGGTTTAATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(79-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.18

96.477

0.542
  pilU Acinetobacter baylyi ADP1

52.661

96.748

0.509
  pilU Vibrio cholerae strain A1552

51.714

94.851

0.491
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.936

92.683

0.379
  pilT Neisseria gonorrhoeae MS11

39.193

94.038

0.369
  pilT Neisseria meningitidis 8013

39.193

94.038

0.369
  pilT Pseudomonas stutzeri DSM 10701

39.244

93.225

0.366
  pilT Vibrio cholerae strain A1552

40.24

90.244

0.363
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.24

90.244

0.363

Multiple sequence alignment