Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   ABX298_RS08895 Genome accession   NZ_CP160798
Coordinates   2039506..2040774 (-) Length   422 a.a.
NCBI ID   WP_367394320.1    Uniprot ID   -
Organism   Pseudomonas sp. X4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2034506..2045774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABX298_RS08880 (ABX298_08880) - 2036139..2037773 (-) 1635 WP_367394319.1 SPOR domain-containing protein -
  ABX298_RS08885 (ABX298_08885) aroB 2037789..2038886 (-) 1098 WP_015272135.1 3-dehydroquinate synthase -
  ABX298_RS08890 (ABX298_08890) aroK 2038983..2039501 (-) 519 WP_025340956.1 shikimate kinase AroK -
  ABX298_RS08895 (ABX298_08895) pilQ 2039506..2040774 (-) 1269 WP_367394320.1 type IV pilus secretin PilQ Machinery gene
  ABX298_RS08900 (ABX298_08900) - 2040698..2041699 (-) 1002 WP_367394476.1 pilus assembly protein PilP -
  ABX298_RS08905 (ABX298_08905) - 2041711..2042247 (-) 537 WP_025340959.1 PilN domain-containing protein -
  ABX298_RS08910 (ABX298_08910) pilM 2042244..2043125 (-) 882 WP_286100710.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45808.66 Da        Isoelectric Point: 4.8419

>NTDB_id=1022073 ABX298_RS08895 WP_367394320.1 2039506..2040774(-) (pilQ) [Pseudomonas sp. X4]
MKTLMKWMLAALLPVPLASATPYQGEPLSLNFQDVEVRSVLQVLADYAGVNLVASDAVQGSVTLRLQDVPWDQALDLVLR
SKGLARRQEGNVLLVAPAAEFAAQSVDARVGQALDAQLQPLRRELLPIHHAKAAELAELLLASLADDGILTGRGSLSVDE
RTNTLVVHLPADRLAELRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYVEDPLPGKDLFVDLGVERVGGSI
GLGLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDN
KVIMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVVDGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=1022073 ABX298_RS08895 WP_367394320.1 2039506..2040774(-) (pilQ) [Pseudomonas sp. X4]
ATGAAAACGTTGATGAAATGGATGCTGGCAGCCCTGCTGCCGGTCCCGCTGGCGTCGGCGACGCCTTATCAGGGTGAACC
ACTGTCGCTGAACTTCCAGGATGTGGAAGTGCGCTCGGTGCTGCAGGTGCTGGCTGACTATGCTGGCGTCAACCTGGTCG
CCAGCGATGCCGTGCAGGGTAGCGTCACCTTGCGCTTGCAGGATGTGCCATGGGACCAGGCGCTGGACCTGGTGCTGCGT
AGCAAGGGCCTGGCCCGGCGCCAGGAGGGCAATGTGCTGCTGGTGGCGCCTGCGGCCGAGTTTGCCGCGCAGTCTGTCGA
TGCCCGTGTGGGCCAGGCACTGGATGCGCAGTTGCAGCCGTTGCGCCGGGAGCTGTTGCCCATCCATCACGCCAAGGCCG
CCGAACTTGCGGAGCTGCTGCTGGCGAGCCTGGCGGATGACGGCATCCTCACCGGTCGGGGCAGCCTGAGTGTCGATGAG
CGCACCAACACGCTGGTGGTACACCTGCCGGCCGACCGCCTTGCCGAGCTGAGGCAACTGGTGGCGCAGCTGGACGTACC
GGTGCGCCAGGTGGCGATCGAGGCGCGTATCGTCGAGGCCAACGTCGATTACGAAAAAAGCCTGGGGGTACGCTGGGGTG
GGCCGCTTTACGTTGAAGACCCGCTGCCGGGCAAGGACCTGTTCGTCGACCTCGGGGTGGAGCGGGTGGGCGGCAGTATC
GGCCTGGGGCTTCTGCGTGGCGATGTGCTGCTCGACCTGGAGCTCAGTGCCATGGAAAAGAGCGGCAACGGCGAAATCAT
CTCGCAGCCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGGATCCTCAAGGGTACCGAGGTGCCGTACCAGGAAACCA
GCAAGAGCGGTGCCACCTCGGTAGCGTTCCGCGAAGCCTCGCTGTCGCTGGAGGTAACCCCGCAGATCACCCCGGACAAC
AAGGTGATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTTCGCGTGGTGGACGGTGAAACGATCGTGATTGGTGGCGTGTACTCGACTTCGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.611
  pilQ Acinetobacter baumannii strain A118

42.516

100

0.464
  pilQ Acinetobacter baumannii D1279779

42.516

100

0.464
  comQ Acinetobacter baylyi ADP1

42.257

100

0.453
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.763

100

0.427
  pilQ Vibrio cholerae strain A1552

41.763

100

0.427
  pilQ Legionella pneumophila strain ERS1305867

42.584

99.052

0.422
  pilQ Legionella pneumophila strain Lp02

42.584

99.052

0.422
  pilQ Vibrio campbellii strain DS40M4

40.741

100

0.417
  pilQ Neisseria gonorrhoeae strain FA1090

36.603

99.052

0.363

Multiple sequence alignment