Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   ABZP20_RS13995 Genome accession   NZ_CP160433
Coordinates   2906126..2907400 (+) Length   424 a.a.
NCBI ID   WP_004633031.1    Uniprot ID   R0E2W3
Organism   Ralstonia sp. CP     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2901126..2912400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP20_RS13975 (ABZP20_13975) ispB 2901618..2902547 (+) 930 WP_049768047.1 octaprenyl diphosphate synthase -
  ABZP20_RS13985 (ABZP20_13985) - 2902820..2904094 (+) 1275 WP_012436459.1 HlyC/CorC family transporter -
  ABZP20_RS13990 (ABZP20_13990) pilB 2904354..2906081 (+) 1728 WP_004633029.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABZP20_RS13995 (ABZP20_13995) pilG 2906126..2907400 (+) 1275 WP_004633031.1 type II secretion system F family protein Machinery gene
  ABZP20_RS14000 (ABZP20_14000) pilD 2907405..2908286 (+) 882 WP_012436460.1 A24 family peptidase Machinery gene
  ABZP20_RS14005 (ABZP20_14005) coaE 2908300..2908914 (+) 615 WP_004633035.1 dephospho-CoA kinase -
  ABZP20_RS14010 (ABZP20_14010) zapD 2909050..2909808 (+) 759 WP_004633038.1 cell division protein ZapD -
  ABZP20_RS14015 (ABZP20_14015) - 2909817..2910020 (+) 204 WP_004633040.1 DNA gyrase inhibitor YacG -
  ABZP20_RS14020 (ABZP20_14020) - 2910013..2910474 (-) 462 WP_037022392.1 NUDIX domain-containing protein -
  ABZP20_RS14025 (ABZP20_14025) - 2910452..2911336 (-) 885 WP_012436462.1 ATP-binding protein -

Sequence

Protein


Download         Length: 424 a.a.        Molecular weight: 46661.83 Da        Isoelectric Point: 10.3665

>NTDB_id=1021101 ABZP20_RS13995 WP_004633031.1 2906126..2907400(+) (pilG) [Ralstonia sp. CP]
MATRAPAANRAAAAPNRTTGKTAKTKAPTQYIFEWEGKDRKGKIFKGEMRAESITEVNAILRKQGLSITKSKRRRAARGK
KITPKDIAYFTRQLSTMLKAGVPLLQSIDIIAKGHANPNFTQLLTEIRVDIESGSSMAQAFRRHPKYFDTLYCNLIDAGE
QGGILDALLERLSLYMEKSLALKAQIKSALIYPISVLTVAFLVTVVLMIFVVPSFKGVFSGFGADLPAPTLFVIAISDFF
VKWWIPIVLGPIAGIALFSRAFKRSENVQRSTHRFILKIPIFGDIIRKATIARWTRTLATMFAAGTPLVESMESVAGAAG
NWIYHDATLEVQQAVRIGTSLTNAMQATHVFDNMVLQMTQIGEESGALDNMLLKVAEFYEREVDDAVANISTLIEPIIIV
FLGVLIGGMVVAMYLPIFKLGTVV

Nucleotide


Download         Length: 1275 bp        

>NTDB_id=1021101 ABZP20_RS13995 WP_004633031.1 2906126..2907400(+) (pilG) [Ralstonia sp. CP]
ATGGCCACACGAGCACCCGCCGCGAACCGCGCGGCCGCAGCGCCCAATCGCACGACCGGCAAGACCGCCAAGACCAAGGC
GCCCACGCAGTACATCTTCGAGTGGGAAGGCAAAGATCGGAAAGGCAAGATCTTCAAGGGCGAGATGCGCGCCGAGAGCA
TCACCGAGGTGAATGCCATCCTGCGCAAGCAAGGCCTGTCGATCACCAAATCCAAGCGCCGCCGCGCGGCCCGCGGCAAG
AAGATCACGCCGAAGGACATCGCCTACTTCACGCGCCAGCTGTCGACCATGCTGAAGGCCGGCGTGCCGCTGCTGCAGTC
GATCGACATCATCGCAAAGGGCCACGCCAACCCGAACTTCACGCAACTGCTGACGGAAATCCGCGTCGATATCGAGTCGG
GCAGCAGCATGGCGCAAGCCTTCCGCCGTCACCCGAAGTACTTCGACACGCTGTACTGCAACCTGATCGACGCGGGCGAG
CAAGGCGGTATTCTGGATGCGCTGCTCGAGCGGCTTTCGCTGTACATGGAAAAGTCCCTCGCACTGAAGGCGCAGATCAA
GTCGGCCCTGATCTACCCGATCTCGGTGCTGACGGTGGCCTTCCTAGTGACCGTGGTGCTGATGATCTTTGTGGTGCCGT
CGTTCAAGGGCGTTTTCTCCGGTTTCGGTGCCGATTTGCCAGCCCCGACGCTGTTCGTCATTGCGATATCGGATTTCTTC
GTCAAGTGGTGGATACCGATCGTGCTGGGCCCCATCGCCGGCATTGCACTGTTTTCGCGCGCATTCAAGCGATCTGAAAA
CGTCCAGCGTTCGACGCATCGCTTCATCCTGAAGATTCCCATCTTTGGCGACATCATCCGCAAGGCAACCATTGCTCGCT
GGACGCGTACGCTGGCTACCATGTTCGCAGCCGGTACGCCGCTCGTGGAGTCGATGGAGTCGGTCGCCGGGGCCGCAGGC
AACTGGATCTACCATGATGCGACCCTCGAGGTTCAGCAAGCGGTACGTATCGGCACGAGTCTCACCAACGCCATGCAAGC
GACACACGTGTTCGACAACATGGTCCTGCAGATGACGCAGATCGGTGAAGAGTCCGGCGCCCTGGACAACATGCTACTGA
AGGTGGCGGAGTTCTACGAGCGCGAGGTGGACGATGCGGTCGCCAACATCTCGACTCTGATCGAACCCATCATCATCGTC
TTCCTCGGCGTGCTAATCGGCGGCATGGTGGTGGCGATGTATTTGCCGATCTTCAAGCTTGGTACGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(90-213)

Type II secretion system (T2SS), protein F (T2SSF)

(294-416)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB R0E2W3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

50.873

94.575

0.481
  pilG Neisseria meningitidis 44/76-A

50.623

94.575

0.479
  pilC Pseudomonas stutzeri DSM 10701

49.751

94.811

0.472
  pilC Legionella pneumophila strain ERS1305867

47.772

95.283

0.455
  pilC Acinetobacter baylyi ADP1

46.683

95.991

0.448
  pilC Acinetobacter baumannii D1279779

46.683

95.991

0.448
  pilC Vibrio campbellii strain DS40M4

40.389

96.934

0.392
  pilC Vibrio cholerae strain A1552

40.602

94.104

0.382

Multiple sequence alignment