Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABZP20_RS13990 Genome accession   NZ_CP160433
Coordinates   2904354..2906081 (+) Length   575 a.a.
NCBI ID   WP_004633029.1    Uniprot ID   A0AAD2BV45
Organism   Ralstonia sp. CP     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2899354..2911081
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABZP20_RS13960 (ABZP20_13960) obgE 2899381..2900475 (-) 1095 WP_012436457.1 GTPase ObgE -
  ABZP20_RS13965 (ABZP20_13965) rpmA 2900671..2900931 (-) 261 WP_004633021.1 50S ribosomal protein L27 -
  ABZP20_RS13970 (ABZP20_13970) rplU 2900969..2901280 (-) 312 WP_004633023.1 50S ribosomal protein L21 -
  ABZP20_RS13975 (ABZP20_13975) ispB 2901618..2902547 (+) 930 WP_049768047.1 octaprenyl diphosphate synthase -
  ABZP20_RS13985 (ABZP20_13985) - 2902820..2904094 (+) 1275 WP_012436459.1 HlyC/CorC family transporter -
  ABZP20_RS13990 (ABZP20_13990) pilB 2904354..2906081 (+) 1728 WP_004633029.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABZP20_RS13995 (ABZP20_13995) pilG 2906126..2907400 (+) 1275 WP_004633031.1 type II secretion system F family protein Machinery gene
  ABZP20_RS14000 (ABZP20_14000) pilD 2907405..2908286 (+) 882 WP_012436460.1 A24 family peptidase Machinery gene
  ABZP20_RS14005 (ABZP20_14005) coaE 2908300..2908914 (+) 615 WP_004633035.1 dephospho-CoA kinase -
  ABZP20_RS14010 (ABZP20_14010) zapD 2909050..2909808 (+) 759 WP_004633038.1 cell division protein ZapD -
  ABZP20_RS14015 (ABZP20_14015) - 2909817..2910020 (+) 204 WP_004633040.1 DNA gyrase inhibitor YacG -
  ABZP20_RS14020 (ABZP20_14020) - 2910013..2910474 (-) 462 WP_037022392.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63666.45 Da        Isoelectric Point: 7.1546

>NTDB_id=1021100 ABZP20_RS13990 WP_004633029.1 2904354..2906081(+) (pilB) [Ralstonia sp. CP]
MTLGLALAQSRRIAPTLLTQLEQSAREKRTQLIDELVGSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPAVLSANKHF
AQLRLLPLGRRENRLILATSDPSDPKPIEELRQKMNVAIETVIVEHDKLVRQLSLANEAPAEIKSLFPKQEATQIEYDAG
AAAATRRTTPDGIDDAPVVRFLQKLFTEALLRGASDLHFEPFETFYRVRFRIDGILAQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDRKDKDNKETIERAIDFRVSTLPTLFGEKIVIRILESSTERLDIDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKDCKKPGPLPKETL
LDAGFTEADLDGSWQPYHPVGCETCNGSGYKGRVGIYQVMPITEAIQEIILTHGTALQIAEQARKDGVMSLRESGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1021100 ABZP20_RS13990 WP_004633029.1 2904354..2906081(+) (pilB) [Ralstonia sp. CP]
ATGACACTCGGACTTGCTCTAGCGCAGAGCCGGCGGATCGCGCCGACGCTACTTACCCAACTCGAACAGAGCGCGCGTGA
AAAACGCACGCAACTGATCGACGAACTGGTGGGCAGCGGGATCATGAGTGCGCACGACCTCGCCGTGTTTGTGGCCGGCA
AGTACCAGATGCCGCTGCTCGACCTTGCGCAGTACAAGCTCGACACCGTGCCGGCCGTGCTGTCGGCCAACAAGCACTTT
GCCCAGTTGCGCCTGTTGCCGCTGGGCCGTCGCGAGAACCGCCTCATCCTCGCCACGTCCGACCCGAGCGATCCGAAGCC
GATCGAAGAGCTGCGTCAGAAGATGAACGTAGCCATCGAAACGGTCATCGTCGAACACGACAAGCTGGTTCGCCAACTCA
GCCTCGCCAATGAGGCGCCGGCCGAGATCAAGTCGCTCTTCCCCAAGCAGGAGGCCACGCAGATCGAATACGATGCGGGC
GCTGCCGCCGCCACGCGTCGCACCACCCCCGACGGCATCGACGATGCCCCTGTGGTGCGCTTCCTGCAGAAGCTCTTTAC
CGAAGCCTTGCTGCGCGGCGCATCGGACTTGCATTTCGAGCCCTTTGAAACGTTTTACCGCGTACGTTTCCGCATCGACG
GCATCCTCGCGCAGGTCGCGCAGCCGCCGCTGGATATCCGCGACAAGATCGCGACCCGCATCAAGGTGCTCTCGCGCCTG
GACATCTCGGAAAAGCGCGTGCCGCAAGACGGCCGCATGAAGCTGCTCATCACCGTCCCGAAAGACCGCAAGGACAAGGA
CAACAAAGAGACGATCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTGTTCGGCGAAAAGATCGTGATCC
GGATTCTGGAGTCGTCCACCGAGCGGCTCGATATCGACCAGCTTGGCTATGAGCCGGAGCAAAAGCAGTTGCTGCTCGAC
GTAATCAAGCGGCCGTACGGCATGGTGCTGGTGACGGGGCCAACCGGATCGGGCAAAACCGTGTCGCTGTACACGTTCCT
GAACAGGCTGAACCAGGGCGACATCAATATCTCCACCGCCGAAGACCCAGCGGAAATCCAGTTGCCCGGCATCAACCAGG
TCAACGTGAACGACAAGGCTGGGCTGACCTTTGCGGCGGCGCTGAAGTCGTTCCTGCGGCAGGATCCGGACATCATCATG
GTGGGCGAAATCCGAGATCTGGAGACGGCCGACATCTCGATCAAGGCTGCGCAGACCGGTCACCTGGTGTTTTCCACGCT
GCACACCAACGACGCACCGACCACACTGACCCGCCTGATGAACATGGGGGTGGCGCCGTTCAACATCGCGTCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGCCGGCTGTGCAAGGATTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTCGACGCGGGTTTTACCGAAGCGGACCTCGACGGCTCCTGGCAGCCGTATCACCCGGTCGGTTGCGAAACCTGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATTACCGAGGCGATCCAGGAAATCATTCTCACGCATG
GCACGGCGCTGCAGATTGCCGAGCAGGCGCGCAAAGACGGCGTGATGTCGCTACGCGAATCGGGCCTGCGAAAAGTGAAG
CAGGGGCTGACTTCCCTCGAGGAAGTGCTGGCCACAACGAACCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

51.681

98.261

0.508
  pilF Neisseria gonorrhoeae MS11

53.704

93.913

0.504
  pilB Acinetobacter baylyi ADP1

50.355

98.087

0.494
  pilB Legionella pneumophila strain ERS1305867

46.41

99.304

0.461
  pilB Vibrio cholerae strain A1552

45.133

98.261

0.443
  pilB Vibrio campbellii strain DS40M4

45.073

95.304

0.43
  pilB Vibrio parahaemolyticus RIMD 2210633

45.018

94.261

0.424
  pilF Thermus thermophilus HB27

38.571

97.391

0.376

Multiple sequence alignment