Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABV408_RS07655 Genome accession   NZ_CP159578
Coordinates   1674792..1676519 (-) Length   575 a.a.
NCBI ID   WP_353981838.1    Uniprot ID   -
Organism   Salinicola endophyticus strain HNIBRBA4755     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669792..1681519
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABV408_RS07630 (ABV408_07630) - 1670586..1671539 (+) 954 WP_353981833.1 Nudix family hydrolase -
  ABV408_RS07635 (ABV408_07635) yacG 1671646..1671897 (-) 252 WP_353981834.1 DNA gyrase inhibitor YacG -
  ABV408_RS07640 (ABV408_07640) coaE 1671894..1672541 (-) 648 WP_353981835.1 dephospho-CoA kinase -
  ABV408_RS07645 (ABV408_07645) pilD 1672538..1673398 (-) 861 WP_353981836.1 A24 family peptidase Machinery gene
  ABV408_RS07650 (ABV408_07650) pilC 1673484..1674710 (-) 1227 WP_353981837.1 type II secretion system F family protein Machinery gene
  ABV408_RS07655 (ABV408_07655) pilB 1674792..1676519 (-) 1728 WP_353981838.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABV408_RS07660 (ABV408_07660) - 1676762..1677241 (+) 480 WP_353981839.1 pilin -
  ABV408_RS07665 (ABV408_07665) - 1677316..1677600 (-) 285 WP_035473735.1 hypothetical protein -
  ABV408_RS07670 (ABV408_07670) - 1677852..1679303 (+) 1452 WP_353981840.1 TrkH family potassium uptake protein -
  ABV408_RS07675 (ABV408_07675) - 1679496..1680698 (-) 1203 WP_353981841.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63262.44 Da        Isoelectric Point: 5.7535

>NTDB_id=1014607 ABV408_RS07655 WP_353981838.1 1674792..1676519(-) (pilB) [Salinicola endophyticus strain HNIBRBA4755]
MPTTTSSGIHRGIARRLIDRGLIEAATCEHALHSAKEAGQTLLEFLVRQGRVDPRQAGEAAAREYGLPFLDLQPVAWERL
PIVEPIAEKLINRFQVLPLARDDRRLTVAVAFPATLAKLDELQFTTGLAIDGVLVPFDQLERALARRLQHQDTSGMLEAL
DEASDAVTLDTSEIDLEEEAAALDAGGDNDAPIVRFVNRVLRDAIQRGASDVHFEPYESSFRVRFRIDGMLLEVTRPPLN
LRTRIAARLKVMARLDISERRLPQDGAMKLRLEDQRAIDFRVNSLPTVFGEKIVLRLLDARATSWGIDALGFSPPQRELF
ESALARPQGMILVTGPTGSGKTVTLYTGLNRLNTEERNISTAEDPVELKIPGINQVNVQTRIGLDFASALRAFLRQDPDV
VMVGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLRNMGLASFNIASSVSLIIAQRLARRLCPHCRVPTQVPDE
VLLGEGITQARLASATLYRPGGCDRCTLGYKGRQGIYEVVPIDDAMSQLIMRDGHALELAQLARERGYPGLRQSAIELAL
SGVTSLEEVNRVTLD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=1014607 ABV408_RS07655 WP_353981838.1 1674792..1676519(-) (pilB) [Salinicola endophyticus strain HNIBRBA4755]
GTGCCGACCACCACCTCCTCCGGCATCCATCGCGGTATCGCCCGCCGGCTGATCGATCGTGGCCTGATCGAGGCGGCGAC
CTGCGAGCACGCGCTGCACTCGGCGAAGGAGGCCGGCCAGACACTGCTCGAGTTCCTGGTGCGCCAGGGCCGGGTCGATC
CGCGCCAGGCCGGTGAGGCGGCGGCACGTGAGTATGGCCTGCCATTTCTCGATCTACAGCCCGTGGCATGGGAACGCCTA
CCGATCGTCGAGCCGATCGCAGAGAAGCTGATCAACCGCTTCCAGGTACTGCCGCTGGCGCGCGACGACCGCCGCCTCAC
GGTGGCTGTCGCCTTCCCCGCCACGCTGGCCAAGCTCGACGAGCTCCAGTTCACCACCGGGCTGGCGATCGATGGCGTGC
TGGTGCCGTTCGATCAACTCGAGCGTGCCCTCGCCCGCCGCCTTCAGCACCAGGACACCTCGGGCATGCTCGAGGCGCTC
GACGAGGCCAGCGATGCGGTGACGCTGGATACCAGCGAGATCGATCTGGAAGAGGAAGCCGCGGCCCTGGATGCTGGCGG
CGACAACGATGCGCCTATCGTGCGCTTCGTCAACCGCGTGCTGCGCGACGCCATCCAGCGCGGCGCTTCGGACGTGCACT
TCGAGCCCTACGAGTCGAGCTTTCGGGTGCGCTTTCGCATCGACGGCATGCTGCTGGAAGTGACCCGCCCGCCGCTCAAC
CTGCGCACCCGCATCGCCGCACGGCTCAAGGTGATGGCACGCCTGGACATCTCCGAGCGCCGCCTGCCCCAGGACGGCGC
GATGAAACTGCGCCTGGAGGACCAGCGCGCCATCGACTTCCGCGTCAACAGCCTGCCCACGGTGTTCGGTGAGAAGATCG
TACTGCGCCTGCTCGACGCCCGCGCCACCAGCTGGGGCATCGACGCGCTCGGCTTCTCACCGCCCCAGCGCGAGCTGTTC
GAGAGTGCGTTGGCGCGCCCCCAGGGGATGATTCTGGTGACCGGCCCCACCGGTAGCGGCAAGACGGTGACCCTCTATAC
CGGGCTCAATCGACTCAATACCGAGGAGCGCAACATCTCCACCGCCGAGGACCCGGTGGAACTCAAGATACCGGGGATCA
ACCAGGTCAACGTGCAGACACGCATCGGGCTCGATTTCGCCAGCGCCCTGCGCGCCTTTCTGCGCCAGGACCCTGACGTG
GTGATGGTCGGCGAGATTCGTGATCTCGAAACCGCCGAGATCGCGGTCAAGGCGGCCCAGACCGGCCACCTGGTGCTCTC
CACACTGCACACCAACTCTGCCGCCGAGACGCTGACGCGGCTGCGCAACATGGGGCTGGCAAGCTTCAACATCGCCAGTT
CGGTGTCACTGATCATTGCCCAGCGCCTGGCGCGTCGGCTCTGCCCACACTGCCGGGTGCCGACTCAGGTGCCTGACGAG
GTGCTGCTCGGCGAAGGTATCACCCAAGCGCGCCTGGCCAGCGCCACGCTCTATCGTCCCGGCGGCTGTGACCGCTGCAC
GCTGGGCTACAAGGGCCGCCAGGGGATCTATGAAGTGGTCCCGATCGATGACGCCATGAGCCAATTGATCATGCGTGATG
GCCACGCCCTGGAACTGGCACAGCTGGCGCGCGAACGCGGCTATCCCGGCCTGCGCCAGAGCGCCATCGAGCTGGCGCTC
AGCGGCGTGACCAGCCTGGAGGAGGTCAACCGCGTGACCCTCGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

51.031

100

0.517
  pilB Acinetobacter baumannii D1279779

51.215

100

0.513
  pilB Legionella pneumophila strain ERS1305867

51.053

99.13

0.506
  pilB Vibrio parahaemolyticus RIMD 2210633

47.676

100

0.482
  pilB Vibrio campbellii strain DS40M4

49.551

96.87

0.48
  pilB Vibrio cholerae strain A1552

48.115

96.87

0.466
  pilF Neisseria gonorrhoeae MS11

46.14

99.13

0.457
  pilB/pilB1 Synechocystis sp. PCC 6803

37.152

100

0.395
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.748

96.696

0.384
  pilF Thermus thermophilus HB27

38.732

98.783

0.383

Multiple sequence alignment