Detailed information NTG1009090

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: ABKA15_RS01295
• Genome accession: NZ_CP157941
• Coordinates: 247789..248226 (+)
• Length: 145 a.a.
• NCBI ID: WP_049513212.1
• Uniprot ID: A0A4U9ZRD8
• Organism: Streptococcus sp. KHUD_010
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 242789..253226

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABKA15_RS01260 (ABKA15_01255)	glnA	242968..244310 (+)	1343	Protein_245	type I glutamate--ammonia ligase	-
ABKA15_RS01265 (ABKA15_01260)	-	244498..244863 (+)	366	WP_049507022.1	DUF1033 family protein	-
ABKA15_RS01270 (ABKA15_01265)	comYA	244933..245874 (+)	942	WP_049513218.1	competence type IV pilus ATPase ComGA	Machinery gene
ABKA15_RS01275 (ABKA15_01270)	comYB	245816..246838 (+)	1023	WP_185952230.1	competence type IV pilus assembly protein ComGB	Machinery gene
ABKA15_RS01280 (ABKA15_01275)	comYC	246835..247152 (+)	318	WP_003028473.1	competence type IV pilus major pilin ComGC	Machinery gene
ABKA15_RS01285 (ABKA15_01280)	comYD	247112..247540 (+)	429	WP_049507639.1	competence type IV pilus minor pilin ComGD	Machinery gene
ABKA15_RS01290 (ABKA15_01285)	comGE/cglE	247512..247805 (+)	294	WP_049513214.1	competence type IV pilus minor pilin ComGE	Machinery gene
ABKA15_RS01295 (ABKA15_01290)	comGF/cglF	247789..248226 (+)	438	WP_049513212.1	competence type IV pilus minor pilin ComGF	Machinery gene
ABKA15_RS01300 (ABKA15_01295)	comGG	248207..248533 (+)	327	WP_264340993.1	competence type IV pilus minor pilin ComGG	-
ABKA15_RS01305 (ABKA15_01300)	comYH	248605..249558 (+)	954	WP_049513210.1	class I SAM-dependent methyltransferase	Machinery gene
ABKA15_RS01310 (ABKA15_01305)	-	249605..250804 (+)	1200	WP_049513208.1	acetate kinase	-
ABKA15_RS01315 (ABKA15_01310)	glmS	251197..253011 (+)	1815	WP_049513230.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16829.39 Da
• Isoelectric Point: 10.0941

>NTDB_id=1009090 ABKA15_RS01295 WP_049513212.1 247789..248226(+) (comGF/cglF) [Streptococcus sp. KHUD_010]
MFKANKVKAFTLLEALIALFVLSGSVLLFQSMTRLLAFEVQARQHSEQREWLLFADQLEAELARSSFEKVENNRLYMKQD
GKVIAFGKSSGQDFRKTNANGKGYQPMVYDVKKAEISQKNELIHVRLTFRRGLEREFVYRVETES

Nucleotide

• Length: 438 bp

>NTDB_id=1009090 ABKA15_RS01295 WP_049513212.1 247789..248226(+) (comGF/cglF) [Streptococcus sp. KHUD_010]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACCCTTTTAGAGGCTCTGATTGCCTTATTTGTACTCAGCGGAAGTGTGCT
GCTTTTTCAGTCAATGACGCGTCTTCTGGCTTTTGAAGTTCAAGCCCGGCAACATAGTGAACAACGAGAATGGCTTTTGT
TTGCTGATCAACTAGAAGCAGAGTTGGCTCGTTCTTCATTTGAAAAGGTGGAGAATAATCGTTTATATATGAAACAGGAC
GGCAAGGTGATTGCATTTGGAAAGTCAAGTGGACAGGATTTCAGAAAAACCAATGCGAATGGGAAAGGTTACCAGCCGAT
GGTTTATGATGTGAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATGTCAGACTGACGTTTAGGCGAGGATTGG
AAAGGGAGTTTGTCTATCGTGTGGAAACAGAAAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A4U9ZRD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae Rx1	65.493	97.931	0.641
comGF/cglF	Streptococcus pneumoniae D39	65.493	97.931	0.641
comGF/cglF	Streptococcus mitis NCTC 12261	65.714	96.552	0.634
comGF/cglF	Streptococcus mitis SK321	65.714	96.552	0.634
comYF	Streptococcus mutans UA159	43.704	93.103	0.407
comYF	Streptococcus mutans UA140	43.704	93.103	0.407
comGF	Lactococcus lactis subsp. cremoris KW2	41.481	93.103	0.386