Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   ABKJ37_RS13245 Genome accession   NZ_CP157386
Coordinates   2778402..2779364 (-) Length   320 a.a.
NCBI ID   WP_348920602.1    Uniprot ID   -
Organism   Enterococcus rotai strain CMTB-CA6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2773402..2784364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABKJ37_RS13210 - 2774333..2775340 (-) 1008 WP_348920596.1 class I SAM-dependent methyltransferase -
  ABKJ37_RS13215 comGG 2775592..2775945 (-) 354 WP_348920597.1 competence type IV pilus minor pilin ComGG -
  ABKJ37_RS13220 comGF 2775935..2776411 (-) 477 WP_348920598.1 competence type IV pilus minor pilin ComGF -
  ABKJ37_RS13225 comGE 2776377..2776700 (-) 324 WP_348920599.1 competence type IV pilus minor pilin ComGE -
  ABKJ37_RS13230 comGD 2776675..2777100 (-) 426 WP_348920600.1 competence type IV pilus minor pilin ComGD -
  ABKJ37_RS13235 comGC/cglC 2777127..2777405 (-) 279 WP_208927102.1 competence type IV pilus major pilin ComGC Machinery gene
  ABKJ37_RS13240 comGB 2777402..2778448 (-) 1047 WP_348920601.1 competence type IV pilus assembly protein ComGB -
  ABKJ37_RS13245 comGA/cglA 2778402..2779364 (-) 963 WP_348920602.1 competence type IV pilus ATPase ComGA Machinery gene
  ABKJ37_RS13250 - 2779889..2781220 (-) 1332 WP_348920603.1 amino acid permease -
  ABKJ37_RS13255 - 2781369..2782079 (-) 711 WP_348920604.1 L-ribulose-5-phosphate 4-epimerase -
  ABKJ37_RS13260 - 2782069..2782929 (-) 861 WP_348920605.1 L-ribulose-5-phosphate 3-epimerase -
  ABKJ37_RS13265 - 2782935..2783573 (-) 639 WP_348920606.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -
  ABKJ37_RS13270 - 2783736..2784035 (-) 300 WP_348920607.1 PTS sugar transporter subunit IIB -

Sequence

Protein


Download         Length: 320 a.a.        Molecular weight: 36956.75 Da        Isoelectric Point: 8.4461

>NTDB_id=1006577 ABKJ37_RS13245 WP_348920602.1 2778402..2779364(-) (comGA/cglA) [Enterococcus rotai strain CMTB-CA6]
MDIKELSLNLIKWGVAECLQDIYILPIENKVQIFTRKGKKRVVFQELNESNGEKLIFHFKFIGGMDVGEKRKAQVGAATY
MINKEAIRLRLSTVGDFRQRESLVIRFLHVFGNSRENYFLPQQLVMVRNQVKRRGLHLFCGPVGSGKTTLMYKLAKETDQ
FQQVITIEDPVEIEEETFLQLQTNSKIDLTYDVLIKACLRHRPDILIIGEIRDGLTAQAAIRAALTGHTVFATIHARSIS
GVYERLAELGVQQREVSECLASIIYQRLLPFRTTKDEEISGLLMDYNFSRKMVSKWSQELRKVWAYGFIDNQTFEEEKED

Nucleotide


Download         Length: 963 bp        

>NTDB_id=1006577 ABKJ37_RS13245 WP_348920602.1 2778402..2779364(-) (comGA/cglA) [Enterococcus rotai strain CMTB-CA6]
ATGGATATTAAAGAACTTTCTCTAAATTTAATTAAGTGGGGTGTTGCTGAATGTTTGCAAGATATCTATATTTTACCTAT
TGAGAATAAGGTTCAGATCTTTACTAGAAAAGGAAAAAAACGAGTAGTTTTTCAAGAACTGAATGAAAGCAACGGTGAAA
AATTGATTTTTCATTTTAAATTTATCGGTGGTATGGACGTTGGTGAAAAAAGAAAAGCGCAGGTGGGGGCTGCAACTTAC
ATGATCAACAAAGAGGCAATACGATTGCGCTTATCGACGGTTGGGGATTTTAGGCAGAGGGAAAGTTTAGTTATTCGTTT
TTTACATGTTTTTGGCAATAGTCGTGAGAATTATTTTTTACCTCAACAATTAGTAATGGTTAGAAATCAAGTTAAACGTC
GTGGCTTGCACTTGTTTTGTGGTCCAGTTGGCTCAGGCAAGACAACCTTGATGTACAAACTTGCAAAAGAAACGGACCAG
TTTCAACAAGTGATTACGATTGAAGACCCCGTTGAAATCGAAGAAGAGACTTTTTTACAGTTACAGACAAATTCTAAAAT
CGATTTGACCTATGATGTATTGATCAAGGCCTGTTTACGTCATCGCCCAGACATTTTAATTATTGGAGAAATTAGAGATG
GGCTTACTGCACAGGCTGCAATCAGAGCGGCACTTACAGGGCATACCGTTTTTGCAACGATACATGCAAGAAGTATTAGC
GGTGTGTATGAACGGTTAGCTGAGTTAGGGGTCCAACAAAGAGAGGTATCAGAATGTTTAGCAAGCATTATTTATCAACG
ATTATTACCGTTTCGGACGACAAAGGATGAAGAAATTAGTGGATTACTGATGGACTATAATTTTTCTAGAAAAATGGTTT
CTAAGTGGTCGCAAGAATTAAGAAAGGTTTGGGCATATGGCTTTATTGATAATCAAACATTTGAGGAAGAAAAAGAAGAT
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(17-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

48.447

100

0.487
  comYA Streptococcus gordonii str. Challis substr. CH1

45.768

99.688

0.456
  comYA Streptococcus mutans UA140

45.201

100

0.456
  comYA Streptococcus mutans UA159

45.201

100

0.456
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

44.201

99.688

0.441
  comGA/cglA/cilD Streptococcus pneumoniae D39

44.201

99.688

0.441
  comGA/cglA/cilD Streptococcus pneumoniae R6

44.201

99.688

0.441
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

44.201

99.688

0.441
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

44.201

99.688

0.441
  comGA Lactococcus lactis subsp. cremoris KW2

44.482

93.438

0.416
  comGA Latilactobacillus sakei subsp. sakei 23K

42.857

87.5

0.375

Multiple sequence alignment